Bovine Trichomoniasis as a Model for Development of Vaccines against Sexually‐transmitted Disease

Corbeil, Lynette B.; Munson, Linda G.; Campero, Carlos Manuel; Bondurant, Robert

doi:10.1111/j.8755-8920.2001.450507.x

Cited by 32 publications

(49 citation statements)

References 17 publications

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“…Luna's stain. Bar ϭ 100 m. thelial inflammatory infiltrates in uterine trichomoniasis, 10 the association with uterine IgE responses has not been reported in trichomoniasis, to our knowledge. Similarly, mast cell and IgE antibodies specific for T. foetus or TF 1.17 antigen have not been reported before.…”

Section: Discussionmentioning

confidence: 71%

“…In our studies of virgin noninfected heifers, lymphoid follicles were not seen in the uterine mucosa but, in infected heifers, accumulations of immunocytes, lymphoid nodules, and follicles (some with germinal centers) were detected under the epithelium and adjacent to infected glands. 2,9,10 We concluded that uterine inductive sites develop after antigenic stimulation. Several questions arise.…”

mentioning

confidence: 86%

“…Results with IgG2 are not presented in this study because the readings were essentially the same as the background (with antigen, but no primary antibody), as in our previous studies. 2,9,10,17 The IgE levels in uterine secretions were detected with a murine monoclonal antibody to bovine IgE as reported previously. 30,40 Because the previous study showed that assays to detect IgE in bovine serum were more sensitive after ammonium sulfate precipitation of other isotypes, we tested this possibility.…”

Section: Antibody Analysismentioning

confidence: 99%

“…35 A related LPG-containing surface antigen has been described by Shaia et al 32 We have shown that systemic or local (intranasal or intravaginal boosts after systemic priming) immunization with an immunoaffinity-purified protective surface antigen (TF1.17 antigen) induces a slight immunoglobulin (Ig)M-specific antibody response but primarily IgA and IgG1 responses in genital secretions and IgG1 antibodies in serum. 5,9,10 Almost no IgG2 antibodies specific for TF1.17 antigen were detected. In cattle, IgG1 responses are believed to be stimulated by Th2 cytokines and IgG2 by Th1 cytokines, 14,15 so there seems to be strong bias for Th2 responses to this antigen.…”

mentioning

confidence: 95%

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Uterine Mast Cells and Immunoglobulin-E Antibody Responses During Clearance ofTritrichomonas foetus

Corbeil

Campero

Rhyan³

et al. 2005

Vet Pathol

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Abstract. We showed earlier that Tritrichomonas foetus-specific bovine immunoglobulin (Ig)G1 and IgA antibodies in uterine and vaginal secretions are correlated with clearance of this sexually transmitted infection. Eosinophils have been noted in previous studies of bovine trichomoniasis but the role of mast cells and IgE responses have not been reported. The hypothesis that IgE and mast cell degranulation play a role in clearance was tested in 25 virgin heifers inseminated experimentally and infected intravaginally with T. foetus strain D1 at estrus and cultured weekly. Groups were euthanatized at 3, 6, 9, or 12 weeks, when tissues were fixed and secretions were collected for culture and antibody analysis. Immunohistochemistry using a monoclonal antibody to a soluble lipophosphoglycan (LPG)-containing surface antigen (TF1.17) demonstrated antigen uptake by uterine epithelial cells. Lymphoid nodules were detected below antigen-positive epithelium. Little IgG2 antibody was detected but IgG1, IgA, IgM, and IgE T. foetus-specific antibodies increased in uterine secretions at weeks 6 and 9 after infection. This was inversely proportional to subepithelial mast cells numbers and most animals cleared the infection by the sampling time after the lowest mast cell count. Furthermore, soluble antigen was found in uterine epithelium above inductive sites (lymphoid nodules). Cross-linking of IgE on mast cells by antigen and perhaps LPG triggering appears to have resulted in degranulation. Released cytokines may account for production of predominantly Th2 (IgG1 and IgE) and IgA antibody responses, which are related to clearance of the infection.

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Section: Discussionmentioning

confidence: 71%

mentioning

confidence: 86%

Section: Antibody Analysismentioning

confidence: 99%

mentioning

confidence: 95%

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Uterine Mast Cells and Immunoglobulin-E Antibody Responses During Clearance ofTritrichomonas foetus

Corbeil

Campero

Rhyan³

et al. 2005

Vet Pathol

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“…Namely, sialomucins and sulfomucins represent the main glycoproteins in the mucus (18), whose pH simulates the pH in the vaginal tract of post-menopausal women (pH is 6-7.5 in cow and as high as 7 in post-menopausal women; 2,19). The important role of bovine mucosa and mucus as in vitro models have been illustrated in several studies addressing topical and systemic drug delivery, adhesion of Lactobacillus to the epithelium, sperm transport through the mucus, and immunization by vaginal route (19)(20)(21)(22)(23)(24)(25)(26). More frequent use of bovine tissue is not just the consequence of the above stated similarities, but also results from the ease of obtaining multiple and large samples on regular basis at relatively low cost.…”

Section: Introductionmentioning

confidence: 99%

Development and Evaluation of an In Vitro Vaginal Model for Assessment of Drug’s Biopharmaceutical Properties: Curcumin

et al. 2012

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Abstract. Vaginal administration is a promising alternative to the per-oral route in achieving systemic or local therapeutic effects, when intestinal drug absorption is hindered by problematic biopharmaceutical drug properties. The aim of this study was to establish an in vitro vaginal model and use it to characterize biopharmaceutical properties of liposomally associated curcumin destined for vaginal delivery. The in vitro permeability, metabolism, and tissue retention of high/low permeable compounds were assessed on cow vaginal mucosa and compared to the permeabilities determined through Caco-2 cells and rat jejunum in vitro. The results showed that the intestinal mucosa was superior to the vaginal one in categorizing drugs based on their permeabilities in high/low permeable classes. Passive diffusion was found to be the main mechanism of drug penetration through vaginal mucosa and it was not affected by transporterenzyme alliance, as their expression/activity was significantly reduced compared to the intestinal tract. Curcumin permeability from the solution form was the lowest of all tested substances due to its significant tissue retention and curcumin-mucus interactions. The permeability of liposomally associated curcumin was even lower but the binding of liposomally associated curcumin to the vaginal tissue was significantly higher. The permeability and tissue retention of liposomal curcumin were vesicle size dependent. Vaginal application of liposomally associated curcumin provides relatively high levels of curcumin in vaginal tissue, with limited systemic absorption.

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Vaccine Development

Healer¹,

Cowman²

2016

Molecular Parasitology

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Bovine Trichomoniasis as a Model for Development of Vaccines against Sexually‐transmitted Disease

Abstract: Systemic immunization or systemic priming with local boosting protects against bovine trichomoniasis via IgG or IgA antibodies (respectively) to a major surface antigen of trichomonads. Immunization of the genital mucosa results in formation of inductive sites for a local IgA response.

Cited by 32 publications

References 17 publications

Uterine Mast Cells and Immunoglobulin-E Antibody Responses During Clearance ofTritrichomonas foetus

Uterine Mast Cells and Immunoglobulin-E Antibody Responses During Clearance ofTritrichomonas foetus

Development and Evaluation of an In Vitro Vaginal Model for Assessment of Drug’s Biopharmaceutical Properties: Curcumin

Vaccine Development

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