Abstract. Novianty R, Saryono, Awaluddin A, Pratiwi NW, Hidayah A, Juliantari E. 2021. The diversity of fungi consortium isolated from polluted soil for degrading petroleum hydrocarbon. Biodiversitas 22: 5077-5084. One of the major problems in the petroleum industry nowadays is crude oil spills. Riau Province, Sumatra is one of the largest oil producers in Indonesia. Accidental releases of petroleum products are of particular concern to the environment. The process of drilling and refining petroleum generates a large amount of oil sludge. One of the effective technologies used in the waste degradation process is bioremediation using certain microorganisms. The prime objective of the current research was to evaluate the efficiency of fungi consortiums in crude oil degradation in Bumi Siak Pusako-Pertamina Hulu, Indonesia. There are three potential fungi isolates as petroleum hydrocarbon degradation agents with four consortium variations. The parameter values of Optical Density (OD), pH, and diluted CO2 were measured on 0, 4, 8, and 16 days. To evaluate the fungal biodegradation activity using Gas Chromatography-Mass Spectrometry (GC-MS). The result showed that consortium II (KF II) has the highest potential to degrade petroleum hydrocarbon (50.61%). The visual GC-MS examination confirmed a decrease in the peak area for eight hydrocarbon compounds, indicating the efficiency of the fungi in the oil decomposition and dismantling of hydrocarbons. Our findings may provide new information on native fungal resources from chronically contaminated terrestrial environments, and will be useful for petroleum-contaminated bioremediation and other industrial applications.
Corona Virus Disease (COVID-19) is announced as pandemic by World Health Organization (WHO) since 11th March 2020. Riau Province has many herbal plants e.g. Cheilocostus specious, Euphorbia hirta l, Cassia torra, Bryophyllum pinnatum, Daniella ensifollia, and Ziziphus mauritiana that can cure many diseases and there is not report yet focusing on in-silico method using SARS-CoV-2 protease (PDB ID:6LU7). This study aims to reveal the potential of compounds contained in Riau Herbal Plants as anti SARS-CoV-2 through its binding to protein receptors. The study was conducted by molecular docking using Autodock Vina 1.5.6 and drug ability studies using Swiss ADME. The docking results of six active compounds including diosgenin, tannin, triterpenoid, chrysophanol, flavone and phytosterol were -8,3; -7,5; -7,5; -7,2; -6,9; -6,9 respectively against the affinity result for natural ligand of COVID-19 (-6,1). This result indicates the stronger bond between ACE2 and inhibitors because the affinity value of active compounds are higher than natural ligand of COVID-19. The SwissADME results show that triterpenoid and tannin violate some Lipinski Rules that make their drug-likeness is low. In general, all the active compounds were potential as candidates of SARS-CoV-2 antiviral but the most potential one is diosgenin in Cheilocostus specious.
Background: Biodegradation of petroleum hydrocarbon needs a specific technique called bioremediation to remove the environmental pollutants. Several indigenous microorganisms including fungi, bacteria, and actinomycetes are effective agents in degrading petroleum derivatives, aliphatic and polyaromatic hydrocarbons (PAHs).Objective: This research aimed to investigate indigenous fungi isolates from petroleum hydrocarbon contaminated soil in Siak which are capable to degrade hydrocarbon.Methods: The competence of indigenous fungi was isolated from a crude oil-contaminated soil which collected from one of oil-field in Siak, Riau. The effectiveness of isolates on the degradation crude oil was tested by culturing the isolates in Bushnell-Haas broth containing crude oil (5% v/v) for 16 days. A decrease in pH, change in optical density and amount of CO2 released were recorded to indirectly indicate the crude oil degradation by the fungi. To measure the percentage of crude oil biodegradation, gravimetric analysis was utilized.Results: The two colonies were selected and identified as Aspergillus sp LBKURCC151 and Penicillium sp LBKURCC153. The results showed that Aspergillus sp LBKURCC151 reached a higher level (61%) of biodegradation after 16 days under the optimum conditions in degrading total petroleum hydrocarbon than Penicillium sp LBKURCC153 (46%).Conclusion: These results indicated that Aspergillus sp LBKURCC151 and Penicillium sp LBKURCC153 are potential degraders for bioremediation in crude oil-contaminated area.
Abstract. Octarya Z, Novianty R, Suraya N, Saryono. 2021. Antimicrobial activity and GC-MS analysis of bioactive constituents of Aspergillus fumigatus 269 isolated from Sungai Pinang Hot Spring, Riau, Indonesia. Biodiversitas 22: 1839-1845. A total of 16 isolates of thermophilic fungi originating from hot springs in Riau and West Sumatra have been tested for their antimicrobial ability against pathogenic microbes Candida albicans, Staphylococcus aureus, and Escherichia coli. The antimicrobial test was carried out by using the disk diffusion method. Molecular identification of the most potential isolate (LBKURCC269) was carried out by amplifying the ITS (Internal transcribed spacer) sequence on rDNA using universal primer ITS-4 and ITS-5. ITS sequence results showed that LBKURCC269 has a 99% similarity to Aspergillus fumigatus. Ethyl acetate extract of LBKURCC269 (Aspergillus fumigatus 269) showed good antimicrobial activities against three pathogenic microbes tested with the inhibition of 17 mm, 13 mm, and 13 mm against Staphylococcus aureus, Candida albicans, and Escherichia coli, respectively. There were 24 identified chemical compounds in ethyl acetate extract. The major compounds were eicosane, eicosane 2-methyl, phenol, 2,6-bis(1,1-dimethyl ethyl)-4-methyl, hexadecane 2, and 11-octadecenoic acid, methyl ester. These findings suggest that thermophilic fungi isolated from hot springs could serve as reservoirs for new bioactive compounds of industrial and medical importance.
Naftalena merupakan salah satu polutan yang sulit untuk didegradasi karena termasuk kedalam kelompok PAH dan bersifat karsinogenik. Tujuan dari penelitian ini adalah untuk menguji efektifitas isolat fungi indigen dari Provinsi Riau untuk mendegradasi senyawa toksik naftalena dengan penambahan glukosa sebagai kosubstrat pertumbuhan. Degradasi naftalena oleh isolat fungi dipengaruhi oleh beberapa parameter, diantaranya biomassa, pH dan persentase degradasi yang diukur pada 0, 4, 8, 12 dan 16 hari inkubasi. Berdasarkan dari parameter biomassa isolat fungi Penicillium sp. LBKURCC153 mengalami fase eksponensial sampai hari ke-16. Pada fase eksponensial terjadi pertambahan jumlah sel secara maksimum yang menunjukkan respon positif terhadap penggunaan glukosa dan naftalena sebagai sumber karbon dan energi. Isolat mengalami penurunan pH dari 7 sampai 3,8 yang artinya ada aktivitas pertumbuhan yang menghasilkan asam-asam organik dari proses degradasi naftalena. Isolat juga mampu mendegradasi naftalena sebesar 27,5% oleh Penicillium sp. LBKURCC153 pada media dengan penambahan glukosa pada waktu optimum selama 4 hari inkubasi. Kesimpulannya, penambahan kosubstrat glukosa dapat meningkatkan persentase degradasi naftalena oleh Penicillium sp. LBKURCC153
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