Effectiveness of crude oil degrading fungi isolated from petroleum hydrocarbon contaminated soil in Siak, Riau

Abstract

Background: Biodegradation of petroleum hydrocarbon needs a specific technique called bioremediation to remove the environmental pollutants. Several indigenous microorganisms including fungi, bacteria, and actinomycetes are effective agents in degrading petroleum derivatives, aliphatic and polyaromatic hydrocarbons (PAHs).Objective: This research aimed to investigate indigenous fungi isolates from petroleum hydrocarbon contaminated soil in Siak which are capable to degrade hydrocarbon.Methods: The competence of indigenous fungi was isolated from a crude oil-contaminated soil which collected from one of oil-field in Siak, Riau. The effectiveness of isolates on the degradation crude oil was tested by culturing the isolates in Bushnell-Haas broth containing crude oil (5% v/v) for 16 days. A decrease in pH, change in optical density and amount of CO2 released were recorded to indirectly indicate the crude oil degradation by the fungi. To measure the percentage of crude oil biodegradation, gravimetric analysis was utilized.Results: The two colonies were selected and identified as Aspergillus sp LBKURCC151 and Penicillium sp LBKURCC153. The results showed that Aspergillus sp LBKURCC151 reached a higher level (61%) of biodegradation after 16 days under the optimum conditions in degrading total petroleum hydrocarbon than Penicillium sp LBKURCC153 (46%).Conclusion: These results indicated that Aspergillus sp LBKURCC151 and Penicillium sp LBKURCC153 are potential degraders for bioremediation in crude oil-contaminated area.

Removal of Total Petroleum Hydrocarbon (TPH) crude oil by consortium bacteria acetobacter tropicalis and lactobacillus casei

“…The variation of the 7-day contact time showed a decrease in pH to 6.89, the pH for the 14-day contact time decreased to 6.47, and the pH for the 21-day contact time decreased to 5.53. The decrease in pH occurs because the metabolic activity of bacteria that produce organic acids can lower the pH value during the degradation process [30,31]. The pH value is one of the important parameters that characterize the life of bacteria, and fluctuating pH values indicate that bacteria carry out a metabolic condition to be able to grow in their environment [32].…”

Biotransformation of dioxins by assembling RW1 upper pathway gene cassettes in Escherichia coli

“…Which indicates that catechol was formed only in the supernatant of the cells containing dxnB3 and that catechol was cleaved by DbfB2 into 2-hydroxymuconic semialdehyde, the yellow-colored product. Microorganisms such as bacteria and fungi are more effective in degrading polyaromatic hydrocarbons and contaminants removal from the environment (Sari et al 2019;Cao et al 2020). Microbial-based bioremediation is one of the most recent strategies due to the incredible metabolic ability to metabolize a wide variety of organic materials and their ability to resist harsh environmental conditions (Novianty et al 2022;Yamini and Rajeswari 2023).…”

Hydrocarbon Degradation Potentials of Fungi: A Review

“…Bioemulsifiers are biosurfactants with high molecular weight biopolymers of polysaccharides, proteins, lipopolysaccharides, lipoproteins, or even a mixture of these compounds which are produced after fungal metabolisms of petro-hydrocarbons [32,35,36]. Production of emulsifiers by several fungi was previously reported, and their application in the oil and gas industry was investigated [2,29,30,31,35]. Bioemulsifiers are amphipathic molecules that stabilized and enabled the formation of oil-in-water or water-in-oil emulsions [36,37].…”