The study of antibacterial activity of oil from Tamarindus indica Linn seed and fatty acids was conducted in this research. Oil of this plant seed was isolated by extraction and fractionation. Constituents of fatty acid in the oil identified as methyl ester fatty acids. The methyl ester fatty acid was carried out by trans-esterification with methanol/BF3. The methyl ester was identified by gas chromatography and mass spectrometer (GC-MS). There are 13 fatty acids in the Tamarindus indica seed oil including octanoic, decanoic, dodecanoic, tetradecanoic, hexadecanoic, octadecanoic, eicosanoic, docosanoic, tetracosanoic, 11-octadecenoic, 11-eicosenoic, 9,12-octadecadienoic, and 9-octadecenoic acids. The transformation of tamarind seed oil into fatty acids was carried out through hydrolysis with potassium hydroxide solution followed by acidification with a hydrochloric acid solution. The physical and biological properties of the seed oil and fatty acids against antibacterial activity are reported in this paper.
The chemical composition of three cajuput essential oils of Melaleuca cajuputi (M. cajuputi) from different area in Indonesia were analyzed using gas chromatography-mass spectrometry to determine the similarities and differences among their volatile chemical compositions. These cajuput oils come from Malang (East Java), Buru Island (Maluku), and Merauke (Papua). It contains 30 components of which 26 components have been identified. Cajuput oils in this research contained significant quantities of 1,8-cineole (42-60%), α-terpineole (4-18%), caryophyllene (0.6-11%), and α-pinene (3-12%). Oil from Malang (East Java) also contained significant amounts of α-selinene (6.73 %) and accompanied by lesser amounts of sesquiterpene detected only in this oil: valencene, germacrene B, ledol, globulol, epiglobulol, and viridiflorol. The hemiterpene compound, 2,4pentanediol (11.44%), was only found in oil from Buru Island. Oil originating from Papua also contained α-gurjunene (17.31%) as the major component. Cajuput oil from Malang (East Java) has the highest number of chemical components than the others.
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