Study of antibacterial activity of Tamarindus indica Linn seed oil and its fatty acids

Abstract

The study of antibacterial activity of oil from Tamarindus indica Linn seed and fatty acids was conducted in this research. Oil of this plant seed was isolated by extraction and fractionation. Constituents of fatty acid in the oil identified as methyl ester fatty acids. The methyl ester fatty acid was carried out by trans-esterification with methanol/BF3. The methyl ester was identified by gas chromatography and mass spectrometer (GC-MS). There are 13 fatty acids in the Tamarindus indica seed oil including octanoic, decanoic, dodecanoic, tetradecanoic, hexadecanoic, octadecanoic, eicosanoic, docosanoic, tetracosanoic, 11-octadecenoic, 11-eicosenoic, 9,12-octadecadienoic, and 9-octadecenoic acids. The transformation of tamarind seed oil into fatty acids was carried out through hydrolysis with potassium hydroxide solution followed by acidification with a hydrochloric acid solution. The physical and biological properties of the seed oil and fatty acids against antibacterial activity are reported in this paper.

Tamarind seed: Composition, applications, and value addition: A comprehensive review

“…The color of oil is dark yellow (Ajayi et al, 2006); greenish (Panchal et al, 2014) and brownish yellow (Sutrisno et al, 2019). The reported lowest and highest values of specific gravity of oil were 0.849 (Sutrisno et al, 2019)…”

“…Many researchers have also reported oil yield of tamarind seed from various regions. The continuous soxhlet extraction technique was used for tamarind seed oil extraction with polar and non‐polar solvents like petroleum ether ( Ajayi et al, 2006; Panchal et al, 2014), hexane (Fernández et al, 2019; Panchal et al, 2014; Sutrisno et al, 2019), chloroform, and methanol (Panchal et al, 2014). Morad et al (1978) observed the yield to be 16.25% using petroleum ether as solvent; however, other studies have reported the yield in the range of 3.0%–10.91% using ether, hexane, or methanol/chloroform combinations (Ajayi et al, 2006; Andriamanantena et al, 1983).…”

“… Note : Oil extraction solvent used by researchers – Chloroform‐methanol (Pitke et al, 1977); Petroleum ether (Ajayi et al, 2006); Ethanol (Panchal et al, 2014), and Hexane (Sutrisno et al, 2019). …”

“…As observed from the Table 10, the oil is found to be in a liquid state at room temperature. The color of oil is dark yellow (Ajayi et al, 2006); greenish (Panchal et al, 2014) and brownish yellow (Sutrisno et al, 2019). The reported lowest and highest values of specific gravity of oil were 0.849 (Sutrisno et al, 2019) and 0.911 ± 0.4 (Panchal et al, 2014).…”

“…The color of oil is dark yellow (Ajayi et al, 2006); greenish (Panchal et al, 2014) and brownish yellow (Sutrisno et al, 2019). The reported lowest and highest values of specific gravity of oil were 0.849 (Sutrisno et al, 2019) and 0.911 ± 0.4 (Panchal et al, 2014). The saponification value represents the average molecular weight of the oil's triglycerides.…”