Citation of This Article AbstractThis study was conducted to assess the effects of different doses of butaphosphan-cyanocobalamin combination onbody condition score (BCS), betahydroxybutyrate (BHBA) and reproductive parameters in cows with subclinical ketosis (SCK). Holstein-Friesen cows (n=544) were checked for BHBA concentration. Cows with SCK (n=53, BHBA ranging from 1.00 to 3.00 mmol/L) were assigned randomly to receive saline (C0, n=18), 5 mL/100 kg BW (C5, n=18) or 10 mL/100 kg BW (C10, n=17) butaphosphan-cyanocobalamin combination. BHBA concentration was measured on d 0, 10, and 18 relative to treatment application. BCS was evaluated weekly until d 60 postpartum. For reproductive parameters cows were monitored until d 150 postpartum. The median reduction in blood BHBA concentrations was 28, 57, and 75% for C0, C5, and C10, respectively. NEFA and total bilirubin concentrations were significantly decreased in C10 group. The relative median change in BCS as compared to baseline was 17, 12, and 6% for C0, C5, and C10, respectively. Between d 15 and 25 postpartum uterine involution was completed in 44, 83, and 88% of cows in groups C0, C5, and C10, respectively. Interval from calving to first insemination in group C10 was shorter than control group. Overall pregnancy rate was not different among groups. In conclusion butaphosphan-cyanocobalamin combination decreased severity of hyperketonemia, stimulated uterine involution, shortened time to first insemination and increased pregnancy rate. Keywords: Beta hydroxybutyrate, Butaphosphan, Cyanocobalamin, Subclinical ketosis, Reproductive performance Subklinik Ketosisli Süt İneklerinde Farklı Dozlarda Butafosfan ve Siyanokobalamin Kombinasyonunun Etkisinin Değerlendirilmesi ÖzetBu çalışma, subklinik ketozisli (SCK) süt ineklerinde farklı dozlarda butafosfan-siyanokobalamin kombinasyonunun vücut kondisyon skoru (VKS), betahidroksibütirat (BHBA) ve reprodüktif parametreler üzerine etkilerini değerlendirmek amacıyla gerçekleştirildi. Holştayn-Frizyan inekler (n=544) BHBA yönünden kontrol edildi. SCK'li inekler (n=53, 1.00-3.00 mmol/L BHBA); 4 gün süresince günlük salin uygulanan (C0, n=18) ve canlı ağırlığa 5 mL/100 kg dozunda (C5, n=18) ya da canlı ağırlığa 10 mL/100 kg dozunda (C10, n=17) butafosfan-siyanokobalamin kombinasyonunun uygulandığı gruplara rastgele olarak ayrıldı. BHBA düzeyleri; 0, 10 ve 18. günlerde ölçüldü. VKS postpartum 60. güne kadar haftalık değerlendirildi. Reprodüktif parametreler için inekler doğum sonrası 150. güne kadar takip edildi. Kan BHBA düzeyinde medyan azalma C0, C5 ve C10 grubunda sırasıyla %28, %57 ve %75 olarak gerçekleşti. NEFA ve total bilirübin düzeyleri C10 grubunda anlamlı şekilde azaldı. VKS'un relatif medyan değişimi baseline ile karşılaştırıldığında C0, C5 ve C10 grubunda sırasıyla %17, %12 ve %6 olarak belirlendi. Postpartum 15 ve 25. günler arasında uterus involüsyonu C0, C5 ve C10 gruplarında sırasıyla %44, %83 ve %88 tamamlandı. C10 grubunda buzağılama ilk tohumlama aralığı kontrol grubundan daha kısaydı. Ortalama gebeli...

The aim of the current study was to investigate the relationship between prepartum body condition score (BCS), blood metabolites (Glucose, beta-hydroxybutyrate; BHB), oxidative stress (Malondialdehyde; MDA, Glutathione peroxidase; GSH-Px) in transition period and some reproductive parameters in Holstein dairy cows. Fifty cows were divided into two groups [low-BCS = 2.75-3.0 (n=25); adequate-BCS = 3.25-3.75 (n=25)] according to BCS on the 21st day before expected calving. BCS was also recorded at 0 and +21 days after calving. Blood samples collected at -21, 0 and +21 days from calving for MDA and GSH-Px analysis as well as at -21, +14 and +21 days of postpartum for glucose and BHB. Data on reproductive parameters obtained from farm records were also collected. MDA concentrations were higher in group of low-BCS compared to group of adequate-BCS at -21 and 0 d related to calving (p<0.05). GSH-Px activity was lower in group of low-BCS than adequate one during the transition period (p<0.05). Low-BCS group also showed higher concentrations of BHB at +14 and +21 days after calving. During the transition period, low-BCS group had lower glucose concentrations compared to adequate one (p<0.05). Adequate-BCS group of cows showed shorter intervals of calving to first estrus (p<0.01) and calving to conception (p<0.01). In conclusion, cows with lower BCS at the prepartum period had worse metabolic and oxidative balance during the transition period. This situation also was associated with worse reproductive performance in cows.

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This study details the relationship between maternal plasma oxidant-antioxidant enzymes with colostrum quality, serum gamma glutamyl transferase (GGT), immunoglobulin G (IgG) and IgM concentrations of calves in the different calving seasons. Holstein breed cows between two and eight lactations and their calves were enrolled in the study. Holstein cows calving in winter (n=45) and their calves (n=45) were assigned to the winter group, while cows calving in summer (n=45) and their calves (n=45) were assigned to the summer group. Samples for malondialdehyde (MDA) and glutathione peroxidase (GSH-Px) were collected on day -21±3 before expected calving and also on calving day (Day 0). IgG and the specific gravity of the colostrum were determined after calving. Serum GGT and IgG and IgM were measured before the feeding, with colostrum, of calves (0 hours) and also in the 24 th hour following the feeding of colostrum. Plasma MDA levels at -21±3 and 0 days in the summer cows were determined to be higher. GSH-Px activity was higher in the winter cows. IgG levels and the specific gravity of the colostrum were also higher in the winter cows. Calf IgG levels at the 24 th hour of life were higher in the winter cows. In the winter group, IgM levels at 0 and 24 hours were also higher. While MDA was negatively correlated with IgG, IgM, GGT, IgG and the specific gravity of colostrum, GSH-Px activity had a positive correlation with IgG, IgM, GGT, IgG and the specific gravity of colostrum. The observed differences in plasma MDA, GSH-Px, calf serum IgG and IgM levels, and colostrum quality between both groups suggest a possible seasonal effect. The relationship between maternal oxidant-antioxidant enzymes, colostrum quality, and passive calf immunity revealed that these enzymes could be used as indicators in the evaluation of calf health and colostrum quality.

Bu çal ış man ı n amac ı k ı sa süreli saklanm ış ve suland ı rı lm ış sperman ı n, k ı zg ı nl ı klar ı 11 gün arayla iki kez bir PGF2a ile toplula şt ı r ı lan ve tohumlama öncesi oksitosin uygulanan Akkeçilerde sa ğ layaca ğı gebelik oran ı n ı ara ş t ı rmak ve gebeli ğ in 45. ile 100. günlerinde dölütlere ait baz ı ultrasonografik ölçüleri ortaya koymakt ı r. Bu çal ış mada 6.5-1.5 ya ş l ı keçi ve çepiçler kullan ı lm ışt ı r. Keçiler ikinci PGF2a analogu uygulamas ı ndan 65 saat sonra yapay tohumlama ile tohumlanm ış t ı r. Grup I deki hayvanlar suland ı r ı lm ış taze sperma ile tohumlan ı rken Grup Il dekiler için 5'C de 6 saat süreyle saklanm ış ve suland ı r ı lm ış sperma kullan ı lm ış t ı r. Grup I ve Grup Il deki hayvanlar ı n s ı ras ı yla % 90.48 ve % 93.33 ü ikinci PGF2a analogu uygulamas ı ndan sonra k ı zg ı nl ı k göstermi ş tir; ayn ı gruplarda gebelik oranlar ı s ı ras ı yla % 57.14 ve % 53.33 olarak bulunmu ştur. Sadece bir çepiç hormon uygulamas ı na yan ı t vermi ştir. Gebeli ğ in K ı rkbe ş inci günündeki ultrasonografik muayenenin sonucunda Amniyon kesesi çap ı ve kolumna vertebralis uzunlu ğ u s ı ras ı yla, 49.63±1.72 mm ve 23.06±0.94 mm ; yüzüncü günde yap ı lan muayenede ise karunkula, orbita ve kafatas ı , çaplar ı s ı ras ı yla 22.36±1.03 mm, 10.08±0.56 mm ve 32.03±0.57 mm olarak bulunmu ş tur Anahtar Kelimeler: Keçi, k ı zg ı nl ı klar ı n toplula ş t ı rı lmas ı , PGF2u, ultrasonografi, gebelik tan ı s ı .