Diabetic prevalence is at speedy increase globally. Previous studies stated that other than genetics, factors such as environment, lifestyle, and paternal-maternal condition play critical roles in diabetes through DNA methylation in specific areas of the genome. The purpose of this study is to investigate the methylation pattern of the PDK4 promoter in streptozotocin-induced diabetic mice until the 12th week of the observation. The methylation pattern in the blood samples was analyzed periodically, while the pattern in the muscle sample was only analyzed at the end of the experiment using the blood of the sacrificed animals. Three methylated CpG site 1, CpG site 6, and CpG site 7 were analyzed and quantified based on the band density using bisulfite treatment and methylation-specific polymerase chain reaction (PCR). The hyperglycemia period was developed at the 9th week of experiment. However, there was a significant increase of methylation, specifically on CpG site 6 started from week 6 to week 12. This peculiar methylation on CpG site 6 of PDK4 promoter in the blood sample before the hyperglycemic period might serve as a potential biomarker for early detection of diabetes in the patients. No significant difference was found between the methylation level of streptozotocin (STZ)-treated mice and of the control group in the muscle sample.

quantifications-Nash Clustering and counting the number of Rock-Paper-Scissor cycles-on over one billion match data from Lichess and FICS. Our findings positively indicate that the strategy space occupied by real-world Chess strategies demonstrates a spinning top geometry, and more importantly, there exists a strong connection between the degree of non-transitivity and the progression of a Chess player's rating. In particular, high degrees of non-transitivity tend to prevent human players from making progress on their Elo rating, whereas progressions are easier to make at the level of ratings where the degree of non-transitivity is lower. Additionally, we also investigate the implication of the degree of non-transitivity for population-based training methods. By considering fixed-memory Fictitious Play as a proxy, we reach the conclusion that maintaining large-size and diverse populations of strategies is imperative to training effective AI agents in solving Chess types of games.

Penelitian ini membahas tentang Netflix sebagai salah satu aplikasi streaming digital yang tidak memiliki Badan Usaha Tetap (BUT) dan tidak memiliki NPWP (Nomor Pokok Wajib Pajak) sehingga berdampak pada terganjalnya usaha pemungutan pajak oleh pemerintah. Penelitian ini membahas isu hukum tentang bagaimana pengenaan pajak bagi Netflix yang belum berbentuk badan usaha tetap di Indonesia serta bagaimana peran pemerintah dalam penegakan hukum terhadap pemungutan pajak bagi Netflix yang belum berbadan usaha tetap. Penelitian ini bertujuan untuk menganalisa pengenaan pajak bagi Netflix yang belum berbentuk badan usaha tetap di Indonesia dan mengkaji secara mendalam peran pemerintah dalam penegakan hukum perpajakan dan penagihan pajak terhadap kasus Netflix di Indonesia. Hasil penelitian ini menjelaskan bahwa setiap subjek hukum sebagai wajib pajak wajib pajak yang sumber penghasilannya di wilayah Indonesia harus dikenakan Pajak. Asas ini tercermin pada Penjelasan Pasal 2 ayat (2) Undang-Undang PPh, yang menentukan bahwa, "Subjek pajak badan dalam negeri menjadi Wajib Pajak sejak saat didirikan, atau bertempat kedudukan di Indonesia", serta peran pemerintah dalam hal ini adalah menetapkan kebijakan perpajakan yang akan dicakup dalam RUU Omnibus Law perpajakan.

Sejak jaman dahulu tato merupakan sebuah bentuk kepercayaan, doa dan simbol. Namun, seiring berjalannya waktu, tato di Indonesia membentuk stigma dan pandangan masyarakat menjadi negatif. Namun, seorang tokoh revolusioner tato atau Presiden Tato, Bob Sick, berhasil mencapai keberhasilannya dalam berkarya dengan hasil seni lukisan kontemporer. Penulis memakai teori identitas diri, kreativitas, konsep seni, komunikasi, ideologi, dan stigma sosial. Penelitian ini menggunakan teknik pengumpulan data berupa wawancara terhadap narasumber Ajenk&Munir, Sebastian Lesmana mengenai sejarah karir Bob Sick dan juga komunikasi ideologi Bob Sick melalui tato di sekujur tubuhnya. Penulis menggunakan metode studi kasus. Atas dasar ideologi dari komunikasi Bob Sick melalui tato di sekujur tubuhnya, membuktikan bahwa tato bukanlah stigma negatif, akan tetapi tato merupakan seni dan dapat membuat seseorang lebih ekspresif atau percaya diri.

Penelitian ini dilakukan untuk menguji kendala keuangan dan saling ketergantungan dalam keputusan keuangan. Sampel yang digunakan dalam penelitian ini adalah perusahaan pada sektor consumer goods yang go public dan terdaftar di Bursa Efek Indonesia (BEI) periode 2014-2018. Terdapat 24 perusahaan swasta yang dapat diteliti setelah dilakukan purposive sampling. Arus kas sebagai variabel dependen yang diukur dengan pengeluaran modal, perubahan kas, dividen, perubahan hutang jangka pendek dan panjang dan total aset. Investasi, kas, dan hutang sebagai variabel independen yang diukur menggunakan aktiva tetap bersih, kas, hutang jangka pendek dan panjang, perubahan dalam modal kerja, dividen, nilai pasar dan total aset. Sedangkan arus kas perusahaan dan resiko operasional perusahaan sebagai variabel moderasi. Penelitian ini menggunakan metode least square dan uji individu (uji-t). Hasil penelitian ini menunjukkan bahwa investasi, kas, dan hutang berpengaruh positif terhadap arus kas perusahaan. Implikasi dari penelitian ini bagi manajer keuangan agar perlu memperhatikan jumlah hutang perusahaan karena sangat berpengaruh terhadap arus kas perusahaan. Bagi peneliti selanjutnya sebaiknya menggunakan perusahaan pada sektor lain.  This research was conducted to support finance and interdependence in financial decisions. The sample used in this study was companies in the consumer goods sector that went public and were listed on the Indonesia Stock Exchange (IDX) for the 2014-2018 period. There are 24 companies that can be issued after purposive sampling. Cash flow as the dependent variable needed with capital, cash changes, dividends, short-term loans and total assets. Investment, cash, and debt as independent variables using fixed bonds, cash, short and long term loans, changes in working capital, dividends, market value and total assets. While the company's cash flow and company operational risk as a moderating variable. This research uses the quadratic method and individual test (t-test). The results of this study prove that investment, cash, and loans are positive towards the company's cash flow. The implications of this study for financial managers need to be considered by the number of companies because they need to be considered for the company's cash flow. For further researchers need to use companies in other sectors.

No abstract

Investment policy is the main thing that must prioritize the national interest, not only in the field of new jobs but also must support the domestic eco-sector. In this case the state has an obligation to defend national interests. Specifically in terms of investment by managing contracts or bilateral investment treaty agreements (BIT) based on the Proportionality Principle. This principle is intended to provide justice and certainty for the parties. Bilateral Investment Treaties (BIT) agreements are considered important for the parties, which are related to the agreement. In the Bilateral Investment Treaties Agreement (BIT) are the most preferred clauses of the Nation, the National Treatment and Fair and Equitable Treatment, and the theory of state / government intervention that is considered to be able to balance national interests and protect investors in the mining sector.