The authors measured food reinforcement, polymorphisms of the dopamine D2 receptor (DRD2) and dopamine transporter (DAT1) genes, and laboratory energy intake in 29 obese and 45 nonobese humans 18-40 years old. Food reinforcement was greater in obese than in nonobese individuals, especially in obese individuals with the TaqI A1 allele. Energy intake was greater for individuals high in food reinforcement and greatest in those high in food reinforcement with the TaqI A1 allele. No effect of the DAT1 genotype was observed. These data show that individual differences in food reinforcement may be important for obesity and that the DRD2 genotype may interact with food reinforcement to influence energy intake.

Sebelipase alfa therapy resulted in a reduction in multiple disease-related hepatic and lipid abnormalities in children and adults with lysosomal acid lipase deficiency. (Funded by Synageva BioPharma and others; ARISE ClinicalTrials.gov number, NCT01757184.).

For uptake experiments, 5 to 8 X 105 cells were inoculated into 60 mm petri dishes with 5 ml of MEM containing 1.5 M OH-Cbl and supplemented with 15% fetal calf serum. Two days later the dishes were washed three times with 2 ml of warm medium which consisted of MEM but lacked serum, folic acid, homocysteine, and methionine. The final wash media were replaced with 2 ml of a solution consisting of: 1.6 ml of MEM

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