Increased anthropogenic delivery of nutrients to water bodies, both freshwater and estuarine, has caused detrimental changes in habitat, food web structure, and nutrient cycling. Nitrogen-stable isotopes may be suitable indicators of such increased nutrient delivery. In this study, we looked at the differences in response of macrophyte delta15N values to anthropogenic N across different taxonomic groups and geographic regions to test a stable isotopic method for detecting anthropogenic impacts. Macrophyte delta15N values increased with wastewater input and water-column dissolved inorganic nitrogen (DIN) concentration. When macrophytes were divided into macroalgae and plants, they responded similarly to increases in wastewater N, although macroalgae was a more reliable indicator of both wastewater inputs and water-column DIN concentrations. Smooth cordgrass (Spartina alterniflora Loisel.) Delta15N increased uniformly with wastewater inputs across a geographic range. We used the relationship derived between S. alterniflora and relative wastewater load to predict wastewater loads in locations lacking quantitative land use data. The predictions matched well with known qualitative information, proving the use of a stable isotopic method for predicting wastewater input.
Phototoxicity resulting from photoactivated polycyclic aromatic hydrocarbons (PAHs) has been reported in the literature for a variety of freshwater organisms. The magnitude of increase in PAH toxicity often exceeds a factor of 100. In the marine environment phototoxicity to marine organisms has not been reported for individual or complex mixtures of PAHs. In this study, larvae and juveniles of the bivalve, Mulinia lateralis, and juveniles of the mysid shrimp, Mysidopsis bahia, were exposed to individual known phototoxic PAHs (anthracene, fluoranthene, pyrene), as well as the water-accommodated fractions of several petroleum products (Fuel Oil #2, Arabian Light Crude, Prudhoe Bay Crude, Fuel Oil #6) containing PAHs. Phototoxicity of individual PAHs was 12 to Ͼ50,000 times that of conventional toxicity. Three of the petroleum products demonstrated phototoxicity while the lightest product, Fuel Oil #2, was not phototoxic at the concentrations tested. The phototoxicity of petroleum products appears to be dependent on the composition and concentrations of phototoxic PAHs present: lighter oils have fewer multiple aromatic ring, phototoxic compounds while heavier oils have higher levels of these types of molecules. This study shows that phototoxicity can occur in marine waters to marine species. Further, the occurrence of oil in marine waters presents the additional risk of phototoxicity not routinely assessed for during oil spills.
Abstract-Phototoxicity resulting from photoactivated polycyclic aromatic hydrocarbons (PAHs) has been reported in the literature for a variety of freshwater organisms. The magnitude of increase in PAH toxicity often exceeds a factor of 100. In the marine environment phototoxicity to marine organisms has not been reported for individual or complex mixtures of PAHs. In this study, larvae and juveniles of the bivalve, Mulinia lateralis, and juveniles of the mysid shrimp, Mysidopsis bahia, were exposed to individual known phototoxic PAHs (anthracene, fluoranthene, pyrene), as well as the water-accommodated fractions of several petroleum products (Fuel Oil #2, Arabian Light Crude, Prudhoe Bay Crude, Fuel Oil #6) containing PAHs. Phototoxicity of individual PAHs was 12 to Ͼ50,000 times that of conventional toxicity. Three of the petroleum products demonstrated phototoxicity while the lightest product, Fuel Oil #2, was not phototoxic at the concentrations tested. The phototoxicity of petroleum products appears to be dependent on the composition and concentrations of phototoxic PAHs present: lighter oils have fewer multiple aromatic ring, phototoxic compounds while heavier oils have higher levels of these types of molecules. This study shows that phototoxicity can occur in marine waters to marine species. Further, the occurrence of oil in marine waters presents the additional risk of phototoxicity not routinely assessed for during oil spills.
Narragansett Bay has been heavily influenced by human activities for more than 200 years. In recent decades, it has been one of the more intensively fertilized estuaries in the USA, with most of the anthropogenic nutrient load originating from sewage treatment plants (STP). This will soon change as tertiary treatment upgrades reduce nitrogen (N) loads by about one third or more during the summer. Before these reductions take place, we sought to characterize the sewage N signature in primary (macroalgae) and secondary (the hard clam, Mercenaria mercenaria) producers in the bay using stable isotopes of N (δ 15 N) and carbon (δ 13 C). The δ 15 N signatures of the macroalgae show a clear gradient of approximately 4‰ from north to south, i.e., high to low point source loading. There is also evidence of a west to east gradient of heavy to light values of δ 15 N in the bay consistent with circulation patterns and residual flows. The Providence River Estuary, just north of Narragansett Bay proper, receives 85% of STP inputs to Narragansett Bay, and lower δ 15 N values in macroalgae there reflected preferential uptake of 14 N in this heavily fertilized area. Differences in pH from N stimulated photosynthesis and related shifts in predominance of dissolved C species may control the observed δ 13 C signatures. Unlike the macroalgae, the clams were remarkably uniform in both δ 15 N (13.2±0.54‰ SD) and δ 13 C (−16.76±0.61‰ SD) throughout the bay, and the δ 15 N values were 2-5‰ heavier than in clams collected outside the bay. We suggest that this remarkable uniformity reflects a food source of anthropogenically heavy phytoplankton formed in the upper bay and supported by sewage derived N. We estimate that approximately half of the N in the clams throughout Narragansett Bay may be from anthropogenic sources.
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