Adaptations of wild populations of the estuarine fish Fundulus heteroclitus to persistent environmental contaminants

Nacci, Diane; Coiro, Laura; Champlin, Denise; Jayaraman, Saro; McKinney, Richard A.; Gleason, Timothy R.; Munns, Wayne R.; Specker, Jennifer L.; Cooper, Keith

doi:10.1007/s002270050520

Cited by 178 publications

(230 citation statements)

References 30 publications

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“…Three other studies have examined the PCB concentrations in NBH Fundulus, either in whole fish Lake, 1995 sampled between 1991 and 1993 or in livers Black et al, 1998sampled in 1992Nacci et a!., 1999sampled in 1996. In all of these studies Fundulus were collected in the same area of the harbor the hot spot from which fish were obtained for this study.…”

Section: ±212mentioning

confidence: 99%

“…However, results from studies using labreared offspring of NEll fish Ch. 4 and second generation embryos from NEll fish Nacci et al, 1999 showed that the resistance found in fish from NEH is heritable, strongly suggesting that a genetic mechanism is responsible for this resistance. In the previous chapters, we reported that killifish Fundulus heteroclitus resident in New Bedford Harbor NEll, MA, a federal Superfund site heavily contaminated with PCBs, showed resistance to induction of CYP1A1 following both environmental and laboratory exposures to HAHs compared to killifish from a reference site Scorton Creek, MA, SC Ch.…”

Section: Mechanism Of Resistancementioning

confidence: 99%

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Characterization of resistance to halogenated aromatic hydrocarbons in a population of Fundulus heteroclitus from a marine superfund site

Bello¹

1999

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New Bedford Harbor NBH, MA, is contaminated with halogenated aromatic hydrocarbons HAH including some potent aryl hydrocarbon receptor AhR agonists. To determine ifFundulus heterocitus from NBH have developed resistance to HAll, we examined the inducibility of cytochrome P4501A1 CYP1A1 in fish from NBH and Scorton Creek SC, reference site. Despite higher PCB concentrations in NBH than in SC fish --1 500-fold -CYP1A1 expression, in most tissues, was not higher in NBH fish than in SC fish. Glutathione S-transferase GST and UDP-glucuronosyltransferase UGT activities were higher in NBH fish than in SC fish, but only when fish were collected during different seasons. GST activity was higher in the intestines ofNBH fish than in any other tissue.2,3,7,8-Tetrachlorodibenzoftiran TCDF induced CYP1A1 expression, in all tissues examined, in SC fish. In contrast, NBH fish showed little CYP1A1 induction by any measure, in any tissue. Hepatic GST activity was induced only in male NBH fish. Hepatic UGT activity showed no relationship to treatment in fish from either site.2,3,7,8-Tetrachlorodibenzo-p-dioxin TCDD and J3-naphthoflavone BNF induced CYP1A1 activity to the same level in primary cultures ofhepatocytes from either SC orNBH fish. However, hepatocytes from NBH fish were 14-fold less sensitive to TCDD and 3-fold less sensitive to BNF than hepatocytes from SC fish.To examine the heritability ofresistance, NBH and SC F1 fish were treated with 3H-TCDD or BNF. 3H-TCDD induced CYP1A1 expression only in SC F1 fish. BNF induced CYP1A1 expression in both SC and NBH F1 fish. There was no significant difference in hepatic 3H-TCDD concentrations between SC and NBH F1 fish.Hepatic AhR content, as measured by photoaffinity labeling with '25I-N3Br2DD, was lower in NBH fish than in SC fish and lower in males than in females. After 90 days in captivity, the sex difference persisted, but the site difference did not. TCDF induced hepatic AIIR content in NBH F1 fish.These results indicate that NBH Fundulus have developed a pre-translational, systemic, heritable resistance to HAHs. These findings suggest that an alteration in the AIIR pathway is responsible for this resistance; this is the subject ofcontinuing research. Next, I owe more than I can say to my thesis advisor and friend Mark Hahn. He was always available whenever I needed to consult on ideas, was endlessly tolerant of the bizarre hours I kept, and fostered a truly enjoyable work environment.Bruce Woodin was an ever accommodating and delightful person to work with. I want to thank him for all ofhis help with dissections, his enlightening field trips, and for generally making my time here that much more enjoyable. I thank Diana Franks for her work with the primary culturing of the Fundulus hepatocytes, for her help with fish care and for generally helping to make my life a little easier. Diane Nacci, Ken Rocha, and the EPA laboratory in Narragansette for kindly providing fish. Diane also for her help during the planning stages ofthis work and her willingness to exchange r...

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Section: ±212mentioning

confidence: 99%

Section: Mechanism Of Resistancementioning

confidence: 99%

Characterization of resistance to halogenated aromatic hydrocarbons in a population of Fundulus heteroclitus from a marine superfund site

Bello¹

1999

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“…They are tolerant of significant changes in many environmental conditions, including salinity (Griffith, 1974;Wood and Marshall, 1994;Nordlie, 2006), pH (Gonzalez et al, 1989), temperature (Umminger, 1971;Dunson et al, 1993;Smith and Able, 1994;Nordlie, 2006), and oxygen (Wannamaker and Rice, 2000;Smith and Able, 2003;Stierhoff et al, 2003;Nordlie, 2006). Specific killifish populations have adapted to a variety of anthropogenic stressors, including polycyclic aromatic hydrocarbons (PAH) (Meyer et al, 2002;Ownby et al, 2002;Meyer and Di Giulio, 2003), 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) (Prince and Cooper, 1995a,b), polychlorinated biphenyls (PCB) (Nacci et al, 1999;Bello et al, 2001), and mercury (Weis et al, 1981). For these reasons, killifish have become one of the premier teleost environmental models, and certainly one of the most well-studied estuarine environmental models (Burnett et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

“…Of the killifish populations known to have adapted to environmental contaminants, several have adapted specifically to chemicals that act via AhR-dependent mechanisms, and alterations to the AhR pathway seem to be a critical component of the adaptation (Prince and Cooper, 1995a,b;Nacci et al, 1999;Powell et al, 2000;Bello et al, 2001;Ownby et al, 2002;Bacanskas et al, 2004;Hahn et al, 2004). Specifically, all of these populations exhibit a marked reduction in CYP1A inducibility in response to a variety of AhR agonists (Prince and Cooper, 1995a;Elskus et al, 1999;Nacci et al, 1999;Bello et al, 2001;Arzuaga and Elskus, 2002;Meyer et al, 2002.…”

Section: Introductionmentioning

confidence: 99%

“…Specifically, all of these populations exhibit a marked reduction in CYP1A inducibility in response to a variety of AhR agonists (Prince and Cooper, 1995a;Elskus et al, 1999;Nacci et al, 1999;Bello et al, 2001;Arzuaga and Elskus, 2002;Meyer et al, 2002. To more fully understand alterations to the AhR pathway, CYP1A was chosen as the target for morpholino gene knockdown in killifish from a reference site.…”

Section: Introductionmentioning

confidence: 99%

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Development of the morpholino gene knockdown technique in Fundulus heteroclitus: A tool for studying molecular mechanisms in an established environmental model

et al. 2008

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a b s t r a c t A significant challenge in environmental toxicology is that many genetic and genomic tools available in laboratory models are not developed for commonly used environmental models. The Atlantic killifish (Fundulus heteroclitus) is one of the most studied teleost environmental models, yet few genetic or genomic tools have been developed for use in this species. The advancement of genetic and evolutionary toxicology will require that many of the tools developed in laboratory models be transferred into species more applicable to environmental toxicology. Antisense morpholino oligonucleotide (MO) gene knockdown technology has been widely utilized to study development in zebrafish and has been proven to be a powerful tool in toxicological investigations through direct manipulation of molecular pathways. To expand the utility of killifish as an environmental model, MO gene knockdown technology was adapted for use in Fundulus. Morpholino microinjection methods were altered to overcome the significant differences between these two species. Morpholino efficacy and functional duration were evaluated with molecular and phenotypic methods. A cytochrome P450-1A (CYP1A) MO was used to confirm effectiveness of the methodology. For CYP1A MO-injected embryos, a 70% reduction in CYP1A activity, a 86% reduction in total CYP1A protein, a significant increase in ␤-naphthoflavone-induced teratogenicity, and estimates of functional duration (50% reduction in activity 10 dpf, and 86% reduction in total protein 12 dpf) conclusively demonstrated that MO technologies can be used effectively in killifish and will likely be just as informative as they have been in zebrafish.

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Evolutionary Physiology and Genomics in the Highly Adaptable Killifish ( Fundulus heteroclitus )

Crawford

Schulte

Whitehead

et al. 2020

Comprehensive Physiology

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Adaptations of wild populations of the estuarine fish Fundulus heteroclitus to persistent environmental contaminants

Cited by 178 publications

References 30 publications

Characterization of resistance to halogenated aromatic hydrocarbons in a population of Fundulus heteroclitus from a marine superfund site

Characterization of resistance to halogenated aromatic hydrocarbons in a population of Fundulus heteroclitus from a marine superfund site

Development of the morpholino gene knockdown technique in Fundulus heteroclitus: A tool for studying molecular mechanisms in an established environmental model

Evolutionary Physiology and Genomics in the Highly Adaptable Killifish ( Fundulus heteroclitus )

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