In this laboratory study, Fill Canal, Sealapex, MTA and Portland cement presented antimicrobial activity whilst EndoRez did not.

Purpose:The aim was to evaluate the knowledge of the students of dental students regarding patients care towards HIV positive individuals. Methods:Two hundred and eighty-three dental students (pre-clinical, n = 45; clinical, n = 238) answered an electronic questionnaire, approaching biosafety procedures, oral manifestations of AIDS and knowledge of HIV infection. Data were present as an average from findings from students of nine different semesters, grouping them by pre-clinical (1-4) and clinical (5-9) semesters, from two different university campuses.Furthermore, data were analysed using the t test and chi-square test.

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Introduction and objective:The aim of this study was to evaluate the ability of root canal cleaning of the RaCe rotary instrumentation system (FKG Dentaire – La-Cheaux-de Fonds – Switzerland), compared to manual filing with Stainless Steel K-files (Maillefer Instruments – Ballaigues – Switzerland).Material and Method:Twenty extracted human teeth (maxillary molars) were selected and their pulp tissue was removed after coronal access. The root canals were filled with a dye (India ink) and allowed to dry for 48 hours, followed by establishment of the working length of the mesiobuccal root; then, half of the specimens were instrumented by the modified Oregon technique and the other half were instrumented by crown-down sequence of the RaCe system. After preparation, the teeth were longitudinally sectioned and evaluated according to the amount of remaining dye.Results:Data obtained were registered as numerical scores, and the arithmetic means were compared between groups using the Mann Whitney test. Both techniques were unable to completely clean the interior of the root canals, with a better performance of the manual technique only at the middle third.Conclusion:It could be concluded that the RaCe system was able to provide satisfactory cleaning, similar to that obtained by the manual instrumentation technique.

bjetivo: comparar a qualidade da obturação e a diferença de profundidade de penetração, no interior do canal radicular, dos espaçadores digitais (Finger Spreader), limas tipo Kerr, Flexofile e Nitiflex, quando utilizados como espaçadores, durante a obturação pela Técnica da Condensação Lateral Ativa. Material e Métodos: foram utilizados 40 dentes molares permanentes humanos, extraídos, instrumentados pela Técnica de Goerig Modificada e divididos em quatro grupos. No grupo I foi utilizado o espaçador digital C; no grupo II, lima tipo Kerr; no grupo III, lima Flexofile e, no grupo IV, lima Nitiflex, todos com diâmetro (D 1 ) ou diâmetro da ponta correspondente ao diâmetro de uma lima 30 (0,30mm), para abertura de espaço para colocação dos cones secundários, durante a Técnica da Condensação Lateral Ativa. Avaliou-se e submeteu-se à análise estatística (Kruskal-Wallis e teste de Dunn) a qualidade radiográfica da obturação e a profundidade de penetração dos instrumentos. Resultados e Conclusão: quanto à análise radiográfica da qualidade da obturação, o grupo II foi o que apresentou o melhor resultado, seguido dos grupos I, IV e III, respectivamente. Em relação à profundidade de penetração dos instrumentos, o grupo IV aproximou-se mais da distância desejada, ou seja, 1 milímetro aquém do comprimento de trabalho, seguido, respectivamente, dos grupos II, III e I. UNITERMOS:Obturação do canal radicular; Instrumentos odontológicos; Espaçadores. INTRODUÇÃOA obturação do canal radicular consiste no preenchimento do canal radicular por materiais física e biologicamente compatíveis, visando promover seu selamento o mais hermeticamente possível. Seu sucesso depende de fases preparatórias, da forma como é realizada e dos materiais empregados para a mesma 3 . Para tanto, esses materiais devem apresentar, preferentemente, alguns requisitos como, ser inertes, anti-sépticos e estimular a reparação dos tecidos periapicais, após o tratamento endodôntico 10,12 . A obturação dos canais radiculares até a união cemento-dentina-canal, ou suas proximidades, é um procedimento de grande importância, pois a permanência de um espaço vazio, após uma obturação deficiente, poderia ser comprometedora para os bons resultados que se espera obter do tratamento. Isto 2003; 11(3): 186-91 J Appl Oral Sci

Objective: Evaluate the cytotoxicity in human osteoblastic cells and antimicrobial activity in different root canal sealers in vitro. Methods: BioRoot RCS, TotalFill BC Sealer, and Bio-C Sealer were used in experimental groups, and AH Plus was used as a control. Human osteoblast-like cells and MTT quantitative colorimetric assay were used to evaluate cytotoxicity. Saos-2 cells were exposed to undiluted sealer extracts for 24 h. The supernatant was then collected and the formazan crystals resulting from MTT reduction were dissolved in pure dimethyl sulfoxide. Absorbance was measured in an automated spectrophotometer at a wavelength of 540 nm. Antimicrobial activity was analyzed by the direct contact test using a polymicrobial biofilm composed of Enterococcus faecalis, Candida albicans, and Streptococcus mutans. At 24, 48, and 72 h, colony-forming units were counted on agar plates. A nonparametric Kruskal-Wallis test was used for the statistical analysis. The level of significance was set at 5%. Results: AH Plus showed the lowest cytotoxicity after 24 h, with a significant difference in relation to BioRoot RCS and Bio-C Sealer (p ≤ 0.01). There was no significant difference in cytotoxicity between TotalFill BC Sealer and Bio-C Sealer (p > 0.05). At 24 h, TotalFill BC Sealer and AH Plus showed the least microbial growth compared to Bio-C Sealer (p < 0.05). At 48 and 72 h, there were no significant differences between sealers (p > 0.05). Conclusions: AH Plus had the lowest cytotoxicity. TotalFill BC Sealer and AH Plus yielded greater reductions in microbial counts in the first 24 h compared to Bio-C Sealer. Clinical Relevance: 2c.

Lateral canals are part of the root canal system and may be related to periodontal-endodontic diseases. The filling techniques that employ thermoplasticized gutta-percha have demonstrated better results in filling lateral canals. Objective: The aim of this study was to evaluate, through digital radiographs, three techniques of root canal obturation (Lateral Condensation, Obtura II System, Tagger’s Hybrid) regarding to the ability of filling artificial lateral canals. Material and methods: 30 extracted human lower premolars were used in this study. The crowns were removed and artificial laterals canals were prepared on the coronal, medium and apical thirds of the roots by using a reamer made of a K 10 file. The root canals were instrumented and obturated by the following techniques: Lateral Condensation, Obtura II System, and Tagger’s Hybrid. The teeth were radiographed with IDA digital radiograph system and the filling of the lateral canals was evaluated by scores for further statistical analysis. Results: No significant difference was found in the filling of lateral canals between Lateral Condensation and Obtura II, but a significant difference was found among these two and Tagger’s Hybrid technique. Conclusion: Tagger’s Hybrid technique promoted the highest numbers of obturated lateral canals. In the three groups, the root thirds presenting greater number of filled lateral canals were coronal and middle, regardless of the obturation technique employed.