Penyakit hawar daun tanaman kentang atau yang oleh petani di Kedu, Wonosobo disebut Lodoh merupakanpenyakit yang paling serius di antara penyakit dan hama yang menyerang tanaman kentang di Indonesia. Penyakitlodoh ini disebabkan oleh serangan jamur patogen ganas Phytophthora infestans yang dapat menurunkan produksikentang hingga 90% dari total produksi kentang dalam waktu yang amat singkat. Sampai saat ini kapang patogenpenyebab penyakit busuk batang dan daun tanaman kentang tersebut masih merupakan masalah krusial dan belumada fungisida yang benar-benar efektif terhadap penyakit tersebut. Penelitian ini bertujuan mengoleksi danmengidentifikasi jamur-jamur tanah isolat lokal yang bersifat antagonis terhadap patogen penyebab penyakit busukdaun dan umbi tanaman kentang. Hasil penelitian menunjukkan bahwa penyebab penyakit busuk daun dan umbitanaman kentang di daerah sentra pembibitan tanaman kentang di Kedu Temanggung Jawa Tengah adalahPhytophthora infestans. Terdapat 17 isolat jamur isolat lokal yang dapat diisolasi dari tanah di sentra pembibitantanaman kentang tersebut. Dari 17 isolat jamur ini dapat dikelompokkan menjadi 4 kelompok isolat yang berbedamorfologi koloninya. Pengamatan secara mikroskopis menunjukkan bahwa dari 4 kelompok jamur tanah tersebutadalah dari marga Trichoderma spp, Aspergillus sp, Penicillium sp dan Phytophthora infestans.

Alpha-glucosidase inhibitors are compounds that can prevent the digestion of complex carbohydrates into glucose, so potentially used as a diabetes drug. This study aims to examine the production and extraction of alpha-glucosidase inhibitor compound from Isolate Aktinomiset JP-3 from the sea. The supernatant obtained from the culture of the JP-3 isolate was extracted using various solvents to obtain the active compound. The solvents used were chloroform, methanol, and ethyl acetate. An assay of inhibitor activity of the α-glucosidase using p-nitrophenyl α-D-glucopyranoside substrate. The activity of the enzyme is measured based on the absorbance of p-nitrophenol produced from the breaking reaction of the substrate. The results showed that extraction of alpha-glucosidase inhibitor compound with ethyl acetate yielded extract with highest inhibitor activity. Keywords: alpha-glucosidase inhibitors, actinomycetes, diabetes, extraction, fractionation

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Traditional fermented food as a special delicacy is one of the elements of culinary diversity found in many regions of Indonesia. This type of food is closely related to the customs and surrounding environment, displaying the characteristics of each region and ethnicity. Maluku is one of the regions located in Eastern Indonesia. Ina sua is a simple and traditional fermented food made from fish using salt as a basic ingredient, commonly eaten at a variety of traditional events in Central Maluku, especially in Teon Nila Serua (TNS). Ina sua is made to be stored and consumed during a time when fish is not available and when there are community activities such as birthdays and traditional events. In making Ina sua, fish are salted with salt granules and stored for a certain time. Therefore, this food is famous for its unique taste produced by the fermentation component. In this article, the origin of Ina sua, its method of preparation, and the scientific perspectives surrounding it are discussed.

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Bolivian Rainbow Chili is one of the ornamental chili varieties which is unique in the form of its colorful fruit color. The vegetative growth of ornamental chilies requires additional nutrients and the role of microorganisms to obtain optimal growth. This study aims to determine the optimal growth of rainbow chilies using the application of PGPR biological fertilizer, a combination of PGPR biological fertilizer and NPK synthetic fertilizer, as well as a combination of PGPR biological fertilizer and compost organic fertilizer. This research was conducted with a completely randomized design (CRD). The data obtained were then analyzed by ANOVA and continued with the LSD test and Duncan’s test at the 5% test stage. The results showed that the combination treatment of PGPR and compost or PGPR and NPK provided optimal vegetative growth compared to single PGPR treatment.

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Fusarium oxysporum is a pathogenic fungi for many plants. The fungi have chitin cell wall that can be degraded by chitinase fromchitinolytic bacteria. Aim of this research is determine how the interaction between the bacteria and F.oxysporum. Bacteria were isolatedfrom plant rizosfere. Chitinolytic activity were measured based on the clear zone around the colony in chitin medium. Bacteria and fungiinteraction were determined by an antagonistic test. This research showed that there were 9 chitinolytic bacteria. J4 and P3 had highchitinolytic index, that are 3 and 3.33, respectively. The two isolates antagonist to F.oxysporum, which the bacteria prevent growth of thefungi. The J4 and P3 are alternative biofungicide for F.oxysporum.