Characterization and Pathogenicity of Fusarium oxysporum as the Causal Agent of Fusarium Wilt in Chili (Capsicum annuum L.)

Ferniah, Rejeki Siti; Daryono, Budi Setiadi; Kasiamdari, Rina Sri; Priyatmojo, Achmadi

doi:10.5454/mi.8.3.5

Cited by 15 publications

(10 citation statements)

References 9 publications

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“…It produced cream to orangish pigmentation. Similar descriptions were also reported by ( [4,6,5,13] . Kadam et al (2012) [8] reported that potato dextrose agar supported maximum mycelial growth in different isolates of Fusarium oxysporum f.sp.ciceri.…”

Section: Discussionsupporting

confidence: 89%

Morphological and cultural variability of Fusarium spp. causing wilt in chilli in Karnataka

Gowda¹,

Bhat²,

Nishani³

et al. 2020

Int. J. Chem. Stud.

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Morphological and cultural variability was studied among the isolates of Fusarium spp. collected from fusarium wilt infested chilli fields of north Karnataka. The isolates cultured on potato dextrose agar medium showed considerable cultural variabilities in colony diameter, colony morphology, pigmentation and morphological variabilities in conidial size, septation and sporulation rate. Colony diameter among F. oxysporum isolates ranged from 63.93-89.87 mm and produced white fluffy cottony growth with orangish pigmentation. The isolates produced macro conidia of size 11.5-24 × 2.2-5.5 µm with 3-4 septation, micro conidia of size 5-6 × 2.7-3 µm and chlamydospore of size 10.5-11.2 µm in diameter. Colony diameter among F. solani isolates ranged from 57.80-89.87 mm, and produced white-creamish cottony/fluffy/submersed growth with creamish, light orangish, purplish and pinkish pigmentation. The isolates produced macro-conidia of size 10-25.5×3-6.5 with 2-3 septation, micro-conidia of size 5-12.5×2.1-3.5 µm and chlamydospores of size 10-12 µm in diameter. The isolates also showed variation in sporulation rate.

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Section: Discussionsupporting

confidence: 89%

Morphological and cultural variability of Fusarium spp. causing wilt in chilli in Karnataka

Gowda¹,

Bhat²,

Nishani³

et al. 2020

Int. J. Chem. Stud.

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“…Similarly, different investigations found variation between isolates of Fusarium oxysporum in their virulence (Abada 1994, Attia and Abada 1994and Ferniah et al 2014.…”

Section: Whereasmentioning

confidence: 91%

“…The Fusarium oxysporum Schlecht is associated with wilt disease of sweet pepper and also reported as the causal agent of wilt in sweet pepper (Capsicum annum) (Abada, 1994, Mushtaq and Hashmi 1997, Ferniah et al 2014and Joshi et al 2015. Fusarium wilt group is a vascular fungus caused by a xylem pathogen called F. oxysporum.…”

Section: Whereasmentioning

confidence: 99%

Determination of Heterosis, Gene Action and the Nature of Resistance to Fusarium Wilt Disease (Fusarium Oxysporum F.SP Capsici ) in Sweet Pepper Hybrids

Soliman

Khafagi

2017

Menoufia Journal of Plant Production

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Four parental genotypes from a Center of Germplasm Netherland (CGN) i.e. 1 (PI 23235), 2 (PI 17171), 3 (PI 23096) and 4 (PI 17150) and their six F 1 hybrids in a diallel cross system without reciprocals were used to estimate combining ability (general and specific) and heterosis percentage relative to both mid and better parents and potence ratios for some characters in pepper (Capsicum annuum L.).The experiment was conducted at Kaha Research Farm, Kaliobia Governorate under unheated plastic house during two successive seasons 2015-2016. Survey was conducted at Kaliobia Governorate during 2015 and 2016 and the pathological studies were carried out at the Plant Pathology Research Institute, Agricultural Research center, Giza Governorate. The studies showed that Fusarium oxysporum f.sp capsici (isolate 3) was the highest in frequency and the level of pathogenicity . The susceptibility of 10 genotypes to Fusarium oxysporum f.sp. capsici (isolate 3) indicated that two F 1 crosses 2 × 4 and 3 × 4 were resistant to Fusarium oxysporum f.sp. capsici (isolate 3). The correlation analysis revealed that the disease severity on roots was significantly correlated with four traits; weight/plant (kg), number of fruits /plant, number of days to 50% flower anthesis and fruit length (cm). In contrast, vascular discoloration was significantly correlated with three traits; weight/plant (kg), number of fruits /plant, and fruit length (cm). The 10 genotypes (4 parents and 6 hybrids) were evaluated for yield ;yield components and fusarium wilt resistance to determine the combining ability and heterosis effects.The obtained results reflected that the mean squares for both general combining ability (GCA) and specific combining ability (SCA) were significant for all studied traits except SCA in fruit shape index and fruit flesh thickness, suggesting the presence of both additive and non-additive gene effects in the inheritance of the various studied characters. Estimates of SCA effects showed that the F 1 crosses 2 × 4 and 2 × 3 reflect the highest value in most studied traits; i.e. total yield as weight and number, fruit length and low disease severity of roots and shoots. Hybrid vigour was documented for total yield, as well as, most fruit characters and disease severity. In some crosses, high rate of parent heterosis and potence ratio values were attained for these traits supporting the over dominance hypothesis.

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“…Pathogenic F. oxysporum was isolated from Fusarium wilting chilli plants, which was found in Tawangmangu, Central Java, Indonesia ( Ferniah et al . 2014 ).…”

Section: Methodsmentioning

confidence: 99%

Resistance Response of Chilli (Capsicum annuum L.) F1 to Fusarium oxysporum Involves Expression of the CaChi2 Gene

Ferniah

Kasiamdari

Priyatmojo

et al. 2018

TLSR

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Cross-breeding is a method of producing progeny with better resistance to pathogens. Resistance to pathogens usually involves pathogenesis-related (PR) proteins. Class II chitinase is an example of a defensive PR protein in plants. The class II chitinase in chilli is coded by the CaChi2 gene. In this study, we crossed susceptible with resistant chilli cultivars, analysed the F1 resistance response against pathogenic F. oxysporum, and analysed the level of CaChi2 gene expression in the F1. Data were collected using disease severity index (DSI) determination and gene expression analysis by qRT-PCR (quantitative Reverse Transcriptase Polymerase Chain Reaction). Results showed that the DSI of F1 was not significantly different from the resistant ancestor. The relative CaChi2 expression level of F1 was higher than the susceptible ancestor but not significantly different from the resistant ancestor. We concluded that the F1 can be categorised as resistant to F. oxysporum, and the CaChi2 gene is involved in the molecular defense response.

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Characterization and Pathogenicity of Fusarium oxysporum as the Causal Agent of Fusarium Wilt in Chili (Capsicum annuum L.)

Cited by 15 publications

References 9 publications

Morphological and cultural variability of Fusarium spp. causing wilt in chilli in Karnataka

Morphological and cultural variability of Fusarium spp. causing wilt in chilli in Karnataka

Determination of Heterosis, Gene Action and the Nature of Resistance to Fusarium Wilt Disease (Fusarium Oxysporum F.SP Capsici ) in Sweet Pepper Hybrids

Resistance Response of Chilli (Capsicum annuum L.) F1 to Fusarium oxysporum Involves Expression of the CaChi2 Gene

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