This paper describes the specificity of Aspergillus niger 5-16 alpha-galactosidase toward various oligosaccharides having terminal galactose or stub galactose or both on the oligosaccharide. The galactosidase rapidly hydrolyzed p-nitrophenyl-alpha-D-galactopyranoside, but hardly liberated galactose from melibiose, manninotriose, 6(3)-alpha-D-galactosylmannotriose, etc. On the other hand, the enzyme tore off the stub galactoses attached to the inner mannoses of the main-chain of galactomannooligosaccharides, but not the terminal galactoses attached to the non-reducing-end mannoses of the main-chain. Thus, the substrate specificity of A. niger 5-16 alpha-galactosidase is quite different from that of Mortierella vinacea alpha-galactosidase.
This paper describes the specificity of Aspergillus niger 5-16 alpha-galactosidase toward various oligosaccharides having terminal galactose or stub galactose or both on the oligosaccharide. The galactosidase rapidly hydrolyzed p-nitrophenyl-alpha-D-galactopyranoside, but hardly liberated galactose from melibiose, manninotriose, 6(3)-alpha-D-galactosylmannotriose, etc. On the other hand, the enzyme tore off the stub galactoses attached to the inner mannoses of the main-chain of galactomannooligosaccharides, but not the terminal galactoses attached to the non-reducing-end mannoses of the main-chain. Thus, the substrate specificity of A. niger 5-16 alpha-galactosidase is quite different from that of Mortierella vinacea alpha-galactosidase.
a-Galactosidase (melibiase, a-D-galactoside galactohydrolase, EC 3.2.1.22) from the mycelium of Mortierella vinacea1 ] catalyzes the hydrolysis of terminal a-galactosidic linkages of glycosides. The enzyme is used in the beet sugar industry for decomposing raffinose in beet molasses. For elucidating the substrate specificity of the enzyme, various kinds of a-galactose-containing oligosaccharides are necessary. However, the difficulty of substrate preparation has been an obstacle to the investigation. Vice versa, the enzymehas not been used for the structural analysis of 237 the oligosaccharides. This paper describes the specificity of the a-galactosidase from Mortierella vinacea toward several kinds of galactomanno-oligosaccharides since we were able to prepare the oligosaccharides.
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