a-Galactosidase (melibiase, a-D-galactoside galactohydrolase, EC 3.2.1.22) from the mycelium of Mortierella vinacea1 ] catalyzes the hydrolysis of terminal a-galactosidic linkages of glycosides. The enzyme is used in the beet sugar industry for decomposing raffinose in beet molasses. For elucidating the substrate specificity of the enzyme, various kinds of a-galactose-containing oligosaccharides are necessary. However, the difficulty of substrate preparation has been an obstacle to the investigation. Vice versa, the enzymehas not been used for the structural analysis of 237 the oligosaccharides. This paper describes the specificity of the a-galactosidase from Mortierella vinacea toward several kinds of galactomanno-oligosaccharides since we were able to prepare the oligosaccharides.
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