Biobleaching with Manganese Peroxidase Purified from the Pulp Bleaching Fungus SKB-1152

Kaneko, Reiji; Iimori, Takeshi; Miyawaki, Syoichi; Machida, Makoto; Murakami, Kunio

doi:10.1271/bbb.59.1584

Cited by 15 publications

(4 citation statements)

References 4 publications

(6 reference statements)

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“…During the course of biobleaching and delignification, MnP was the major oxidative enzyme activity detected in the extracellular fluid. Previous observations with other MnP-producing white rot fungi indicate that MnP utilizing Mn(II) as a cofactor is largely responsible for biobleaching of hardwood kraft pulps (2,22,26). Before concluding that this is also the case for Bjerkandera sp.…”

Section: Discussionmentioning

confidence: 82%

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Manganese Is Not Required for Biobleaching of Oxygen-Delignified Kraft Pulp by the White Rot Fungus Bjerkandera sp. Strain BOS55

Moreira

Sierra-Álvarez

Lema

et al. 1997

Appl Environ Microbiol

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The white rot fungus Bjerkandera sp. strain BOS55 extensively delignified and bleached oxygen-delignified eucalyptus kraft pulp handsheets. Biologically mediated brightness gains of up to 14 ISO (International Standards Organization units) were obtained, providing high final brightness values of up to 80% ISO. In nitrogen-limited cultures (2.2 mM N), manganese (Mn) greatly improved manganese-dependent peroxidase (MnP) production. However, the biobleaching was not affected by the Mn nutrient regimen, ranging from 1,000 M added Mn to below the detection limit of 0.26 M Mn in EDTA-extracted pulp medium. The lowest Mn concentration tested was at least several orders of magnitude lower than the K m known for MnP. Consequently, it was concluded that Mn is not required for biobleaching in Bjerkandera sp. strain BOS55. Nonetheless, fast protein liquid chromatography profiles indicated that MnP was the predominant oxidative enzyme produced even under culture conditions in the near absence of manganese. High nitrogen (22 mM N) and exogenous veratryl alcohol (2 mM) repressed biobleaching in Mn-deficient but not in Mn-sufficient culture medium. No correlation was observed between the titers of extracellular peroxidases and the biobleaching. However, the decolorization rate of the polyaromatic dye Poly R-478 was moderately correlated to the biobleaching under a wide range of Mn and N nutrient regimens.

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Section: Discussionmentioning

confidence: 82%

“…Mn(III) organic acid chelates are strong diffusible oxidants capable of oxidizing a large variety of substrates, including phenolic lignin model compounds (16). Mn(II) has been reported to be required for the oxidation of lignin by MnP (48) as well as biobleaching and delignification of hardwood kraft pulp by MnP (26,31).…”

mentioning

confidence: 99%

Manganese Is Not Required for Biobleaching of Oxygen-Delignified Kraft Pulp by the White Rot Fungus Bjerkandera sp. Strain BOS55

Moreira

Sierra-Álvarez

Lema

et al. 1997

Appl Environ Microbiol

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“…Provided that Mn is present, MnP is known to delignify kraft pulp in vitro (19,24,28,43,45) as well as to depolymerize synthetic lignins (18,57). Mn was found to be an obligate mediator substrate of MnP from P. chrysosporium for the oxidation of other substrates (16).…”

Section: Discussionmentioning

confidence: 99%

“…MnPdeficient mutants of the best-characterized biobleaching species, Trametes versicolor, are not able to cause bleaching, and the bleaching activity can be restored by the exogenous addition of MnP (3). Semipurified preparations of MnP have been shown to cause kraft pulp delignification in vitro (19,24,28,43,45). Furthermore, prior extraction of manganese (Mn) from kraft pulp results in the complete loss of biobleaching activity by white rot fungi (22,44).…”

mentioning

confidence: 99%

Role of Organic Acids in the Manganese-Independent Biobleaching System of Bjerkandera sp. Strain BOS55

Moreira

Mester²,

Mayorga³

et al. 1998

Appl Environ Microbiol

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Bjerkandera sp. strain BOS55 is a white rot fungus that can bleach EDTA-extracted eucalyptus oxygen-delignified kraft pulp (OKP) without any requirement for manganese. Under manganese-free conditions, additions of simple physiological organic acids (e.g., glycolate, glyoxylate, oxalate, and others) at 1 to 5 mM stimulated brightness gains and pulp delignification two- to threefold compared to results for control cultures not receiving acids. The role of the organic acids in improving the manganese-independent biobleaching was shown not to be due to pH-buffering effects. Instead, the stimulation was attributed to enhanced production of manganese peroxidase (MnP) and lignin peroxidase (LiP) as well as increased physiological concentrations of veratryl alcohol and oxalate. These factors contributed to greatly improved production of superoxide anion radicals, which may have accounted for the more extensive biobleaching. Optimum biobleaching corresponded most to the production of MnP. These results suggest that MnP from Bjerkandera is purposefully produced in the absence of manganese and can possibly function independently of manganese in OKP delignification. LiP probably also contributed to OKP delignification when it was present.

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Biodegradation by White-Rot Fungi

Ralph¹,

Catcheside

2002

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Biobleaching with Manganese Peroxidase Purified from the Pulp Bleaching Fungus SKB-1152

Cited by 15 publications

References 4 publications

Manganese Is Not Required for Biobleaching of Oxygen-Delignified Kraft Pulp by the White Rot Fungus Bjerkandera sp. Strain BOS55

Manganese Is Not Required for Biobleaching of Oxygen-Delignified Kraft Pulp by the White Rot Fungus Bjerkandera sp. Strain BOS55

Role of Organic Acids in the Manganese-Independent Biobleaching System of Bjerkandera sp. Strain BOS55

Biodegradation by White-Rot Fungi

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