This paper describes data related to a research article titled, “Fas-antisense long noncoding RNA is differentially expressed during maturation of human erythrocytes and confers resistance to Fas-mediated cell death” [1]. Long noncoding RNAs (lncRNAs) are increasingly appreciated for their capacity to regulate many steps of gene expression. While recent studies suggest that many lncRNAs are functional, the scope of their actions throughout human biology is largely undefined including human red blood cell development (erythropoiesis). Here we include expression data for 82 lncRNAs during early, intermediate and late stages of human erythropoiesis using a commercial qPCR Array. From these data, we identified lncRNA Fas-antisense 1 (Fas-AS1 or Saf) described in the research article. Also included are 5′ untranslated sequences (UTR) for lncRNA Saf with transcription factor target sequences identified. Quantitative RT-PCR data demonstrate relative levels of critical erythroid transcription factors, GATA-1 and KLF1, in K562 human erythroleukemia cells and maturing erythroblasts derived from human CD34+ cells. End point and quantitative RT-PCR data for cDNA prepared using random hexamers versus oligo(dT)18 revealed that lncRNA Saf is not effectively polyadenylated. Finally, we include flow cytometry histograms demonstrating Fas levels on maturing erythroblasts derived from human CD34+ cells transduced using mock conditions or with lentivirus particles encoding for Saf.
Acute pancreatitis (AP) is a common medical condition with a wide variety of etiologies. One of the common but frequently undetected causes of acute pancreatitis is microlithiasis, which can appear as biliary "sludge" in the gallbladder on imaging. While a broad workup should be initiated, endoscopic retrograde cholangiopancreatography (ERCP) is the gold standard for the diagnosis of microlithiasis. In this case, we present a severe presentation of acute pancreatitis in a teenager within the postpartum period. A 19-yearold woman presented with severe 10 out of 10 right upper quadrant (RUQ) pain with episodes of nausea that radiated to her back. She had no history of chronic alcoholism, illicit drug use, or over-the-counter supplement use, and no familial history of autoimmune disease, or pancreatitis. The patient was diagnosed with necrotizing acute pancreatitis with gallbladder "sludge" using contrast-enhanced computed tomography (CT) and magnetic resonance cholangiopancreatography (MRCP). She followed up with gastroenterology and had a great clinical recovery. Therefore, it is important to consider acute pancreatitis in patients with idiopathic pancreatitis in their postpartum period as they are prone to forming gallbladder "sludge" which can precipitate and cause a variation in gallbladder pancreatitis which can be difficult to detect on imaging.
Cases of an inguinal bladder hernia (IBH) are rare as the diagnosis may be challenging because patients are often asymptomatic or have nonspecific symptoms. When patients are symptomatic, normally they complain of urinary symptoms. Our patient initially presented to the hospital because he had a ground-level fall after having chest pain while transitioning from a bed to a wheelchair. Incidentally in the emergency department, he was found to have scrotal edema, which was later diagnosed as inguinal bladder herniation. The patient did not have any further episodes of chest pain or abdominal pain once he was given medicinal therapy for his IBH. Surgery is usually the definitive treatment for inguinal bladder herniation, but our patient wished to try medicinal therapy and follow-up outpatient.
Introduction: Insulinomas are the most common cause of hypoglycemia related to endogenous hyperinsulinism. It occurs in 1-4 people per million in the general population. Symptoms include diaphoresis, palpitations, tremors and even confusion or behavioral/personality changes.1 The small size of insulinomas presents a challenge in diagnosis via standard imaging techniques.2 Case Description/Methods: A 47-year-old healthcare worker with a history of goiter presented with 6 months of memory problems and associated lightheadedness, tremors, and blurry vision. She endorsed a 15-pound weight gain in the past month. She also recalled lapses in memory such as forgetting where she parked her car at the grocery store and more dangerously occurring during work when she was not able to remember if she administered medications to patients appropriately. Historical episodes of hypoglycemia had been ongoing for the past 2 years. Baseline labs included a mildy elevated c-peptide, normal IGF-2, TSH, and cortisol, and negative insulin antibody and sulfonyurea screen. Physical examination was normal. MRI showed no pancreatic abnormalities and she was subsequently admitted to our hospital for 72hour inpatient fast. The fast ended at 22 hours due symptomatic hypoglycemia that resolved after administration of glucagon. Labs revealed blood glucose of 42, insulin 9 mU/L, beta-hydroxybutyrate 0.4 mg/dL, C-peptide 1.4 ng/L , proinsulin 40.5 pmol/L which were all diagnostic for insulinoma. CT abdomen showed no enhancing pancreatic masses or metastatic lesions. Thus, the patient underwent endoscopic ultrasound (EUS) fine-needle aspiration which identified an 8x5 mm lesion in the tail of the pancreas. Pathology showed a well-differentiated neuroendocrine tumor, and the patient underwent radiofrequency ablation (RFA). Four months later, the patient was no longer symptomatic and no biochemical evidence of hyperinsulinemia remained on laboratory evaluation. Discussion: Insulinomas remain a diagnostic challenge, due to their rare presentation, nonspecific symptoms and small size. Although most insulinomas are benign with survival rate of 95% they require surgical intervention or radiofrequency ablation to improve survival. Diagnosis of a suspected insulinoma can be made via labs during a supervised, prolonged fast and accurate localization of smaller tumors may require minimally invasive procedures, like EUS. 2
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