Data in support of transcriptional regulation and function of Fas-antisense long noncoding RNA during human erythropoiesis

Abstract

This paper describes data related to a research article titled, “Fas-antisense long noncoding RNA is differentially expressed during maturation of human erythrocytes and confers resistance to Fas-mediated cell death” [1]. Long noncoding RNAs (lncRNAs) are increasingly appreciated for their capacity to regulate many steps of gene expression. While recent studies suggest that many lncRNAs are functional, the scope of their actions throughout human biology is largely undefined including human red blood cell development (erythropoiesis). Here we include expression data for 82 lncRNAs during early, intermediate and late stages of human erythropoiesis using a commercial qPCR Array. From these data, we identified lncRNA Fas-antisense 1 (Fas-AS1 or Saf) described in the research article. Also included are 5′ untranslated sequences (UTR) for lncRNA Saf with transcription factor target sequences identified. Quantitative RT-PCR data demonstrate relative levels of critical erythroid transcription factors, GATA-1 and KLF1, in K562 human erythroleukemia cells and maturing erythroblasts derived from human CD34+ cells. End point and quantitative RT-PCR data for cDNA prepared using random hexamers versus oligo(dT)18 revealed that lncRNA Saf is not effectively polyadenylated. Finally, we include flow cytometry histograms demonstrating Fas levels on maturing erythroblasts derived from human CD34+ cells transduced using mock conditions or with lentivirus particles encoding for Saf.

Upregulation of long noncoding RNA zinc finger antisense 1 enhances epithelial–mesenchymal transition in vitro and predicts poor prognosis in glioma

“…6 However, in recent years, high-throughput transcriptome analysis has revealed that the vast majority (98%) of the human genome can be transcribed into noncoding RNAs. 7 Among them, lncRNAs have acquired extensive attention, recently, as new regulators in many biological processes, such as transcriptional regulation, 8 differentiation, 9 immune response, 10 metabolism, 11 cell growth, and especially tumorigenesis. 12,13 Moreover, since an increasing number of reports have indicated that their abnormal expression is frequently observed in human cancers, some lncRNAs have been validated as biomarkers for diagnostic, metastasis, recurrence, and poor prognosis, [14][15][16][17] such as MALAT-1 for lung, glioma, gastric, and prostate cancer; [18][19][20][21] HOTAIR for breast, ovarian, pancreatic, and colorectal cancer; [22][23][24][25] and CCAT2 for oral squamous cell carcinoma and bladder cancer.…”

Role of Extrinsic Apoptotic Signaling Pathway during Definitive Erythropoiesis in Normal Patients and in Patients with β-Thalassemia

“…During erythropoiesis, Fas-AS1 is upregulated by the erythroid transcription factors GATA-1 and Kruppel-like factor 1 (KLF1) and is negatively regulated by NF-κB. Since the level of Fas-AS1 expression increases during erythroid maturation, it is suggested that the role of Fas lncRNA is to protect developing erythroblasts from Fas-mediated apoptosis via reducing the level of Fas [85,86].…”

Long non-coding RNA homeobox (HOX) A11-AS promotes malignant progression of glioma by targeting miR-124-3p

“…However, in recent years, it has been identified that the vast majority (98%) of the human genome can be transcribed into non-coding RNAs [6]. Accumulating evidence has demonstrated that LncRNAs play critical roles in various biological processes, including transcriptional regulation [7], proliferation [8], differentiation [9], immune response [10], metabolism [11]. In recent years, the roles of LncRNAs in the regulation of tumorigenesis have acquired extensive attention [12,13].…”