Salinity is one of the most significant environmental factors limiting microalgal biomass productivity. In the present study, the model microalga
Chlamydomonas reinhardtii
(
C. reinhardtii
) was exposed to 200 mM NaCl for eight days to explore the physiological, biochemical and metabolomic changes.
C. reinhradtii
exhibited a significant decrease in growth rate, and Chl a and Chl b levels. 200 mM NaCl induced ROS generation in
C. reinhardtii
with increase in H
2
O
2
content. This caused lipid peroxidation with increase in MDA levels.
C. reinhardtii
also exhibited an increase in carbohydrate and lipid accumulation under 200 mM NaCl conditions as storage molecules in cells to maintain microalgal survival. In addition, NaCl stress increased the content of carotenoids, polyphenols and osmoprotectant molecules such as proline. SOD and APX activities decreased, while ROS-scavenger enzymes (POD and CAT) decreased. Metabolomic response showed an accumulation of the major molecules implicated in membrane remodelling and stress resistance such oleic acid (40.29%), linolenic acid (19.29%), alkanes, alkenes and phytosterols. The present study indicates the physiological, biochemical and metabolomic responses of
C. reinhardtii
to salt stress.
The genus Lavandula is known for its different uses in traditional medicine. This study is interested in the chemical composition of Lavandulapedunculata subsp.atlantica (BRAUN-BLANQ.) ROMO as well as evaluating its antibacterial potential against multi-resistant strains. The analysis of Lavandulaatlantica essential oil (LAEO) allows the identification of 47 components representing 93.6 % of all identified. The main constituent of LAEO was camphor (50.4 %), followed by fenchone (14.1 %) and camphene (5.6 %). The antibacterial assays revealed that LAEO was active against all the studied bacteria. A preliminary study of the relationship between certain terpenoids and antibacterial activity was also carried out in order to note the compound(s) that are responsible for LAEO's antibacterial activity. This study showed that the activity of the essential oil may be due to the presence of certain minor compounds such as carvone, considering the presence of the synergistic effect between the essential oil.
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