Amblyomma sculptum (Ixodida: Ixodidae) Berlese, 1888 is the most important tick vector in Brazil, transmitting the bioagent of the most severe form of spotted fever (SF) in part of the Cerrado (in the states of Minas Gerais and São Paulo). In another part of the Cerrado (Central-West region of Brazil), a milder form of SF has been recorded. However, neither the rickettsia nor the vector involved have been characterized. The aim of the current study was to analyse genetic variation and the presence of rickettsia in A. sculptum in Cerrado, from silent areas and with the milder form of SF. Samples were subjected to DNA extraction, amplification and sequencing of 12S rDNA, cytochrome oxidase subunit II and D-loop mitochondrial genes (for tick population analyses), and gltA, htrA, ompA and gene D (sca4) genes for rickettsia researches. Exclusive haplotypes with low frequencies, high haplotype diversity and low nucleotide diversity, star-shaped networks and significant results in neutrality tests indicate A. sculptum population expansions in some areas. Rickettsia amblyommatis, Candidatus Rickettsia andeanae and Rickettsia felis were detected. The A. sculptum diversity is not geographically, or biome delimited, pointing to a different potential in vector capacity, possibly associated with differing tick genetic profiles.

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Background: Having the right identification is essential in the field of entomology. The scanning electron microscopy allows rapid and accurate identification of different species of flies since a better visualization of the external morphology of immature and some adults is possible. The aim of this study is to describe some ultrastructures of three flies in the Mesembrinellidae family: Mesembrinella bellardiana; Mesembrinella bicolor and Mesembrinella semihyalina, all examined by scanning electron microscopy to help increase the anatomical database on flies and deal with phlylogenetic implications. Results: The flies were examined under JEOL 6390LV scanning electron microscope (SEM). The microtrichia morphology of the antennae is similar to that of other and are present on all antennal surface with variable distribution. The chaetic sensilla were observed only in the scape (chaetic sensilla I) and pedicel segments (chaetic sensilla I-III). Three types of sensilla: trichoidea, basiconic and clavate were observed in the first flagellomere of M. bellardiana; and two types of sensilla: trichoidea and basiconic were noticed in M. bicolor and M. semihyalina. Olfactory pits were observed in both gender of M. bellardiana and female of M. bicolor. The maxillary palp of the Mesembrinella species in this study does not show sexual dimorphism. Conclusion: This investigation provided new findings of some diagnostic structures of flies using SEM, since many of them could have not been observed just by the use of light microscopy.

The Rhipicephalus sanguineus (Latreille) complex (Acari:Ixodidae) is composed of species with intra- and interspecific morphological variation that make their diagnosis difficult. In the present study, male specimens of the R. sanguineus complex were collected from dogs in six districts of three regions of Brazil and submitted to molecular and scanning electron microscopy (SEM) analyses. Analysis of COX1 gene, 12S rDNA, and D-loop rDNA shows that ticks classified as R. sanguineus form two different clades. Morphological comparisons using SEM found adult males to exhibit morphological differences in Haller’s organ, festoons, and adanal, spiracular, and genital plates, with the last having potential usefulness in distinguishing male specimens of the complex.

In the field of entomology, right identification of specimens is essential. Scanning electron microscopy (SEM) allows the identification of different species of flies through better visualization of the external morphology of immature and adults. The aim of this study is to describe some ultrastructures of Peckia (Peckia) chrysostoma, all examined by SEM to increase the anatomical database on flies of forensic and public health importance.

Abstract. Four bulb mites deutonymphs (hypopus) from Rhizoglyphus echinopus (Fumouze & Robin) (Acari: Acaridae) were found attached to the head of a subterranean termite belonging to Coptotermes genus in Rio de Janeiro, Brazil. These mite species are commonly associated with ornamental plants and trees with bulbs, corms and tubers. All four hypopus were found attached to the surface of termite's head. Results from this study provided an insight on the phoretic relationship of mites and termites, indicating the role of the latter in the dispersion of the first.Keywords: Acaridae; Arthropods; Dispersion; First record; Phoresy. Ácaros do Bulbo Rhizoglyphus echinopus (Fumouze and Robin) Associados com Cupim Subterrâneo (Isoptera) no BrasilResumo. Quatro deutoninfas de ácaros do bulbo (hipopus) da espécie Rhizoglyphus echinopus (Fumouze & Robin) (Acari: Acaridae) foram encontradas fixadas na cabeça do cupim subterraneo pertencente ao gênero Coptotermes no Rio de Janeiro, Brasil. Estas espécies de ácaros estão comumente associadas a plantas ornamentais e plantas com bulbos e turbéculos. As quatros hipopus foram encontradas fixadas na superfície da cabeça do cupim. Os resultados desse estudo fornecem uma visão sobre a relação forética de ácaros e cupins, indicando o papel deste último na dispersão do primeiro.

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