Detection and preliminary physico-chemical properties of antimicrobial components in the native excretions/secretions of three species of Chrysomya (Diptera, Calliphoridae) in Brazil

Ratcliffe, Norman A.; Vieira, Cecília Stahl; Mendonça, Paloma Martins; Caetano, Rebecca Leal; Queiroz, Margareth Maria de Carvalho; Garcia, Elói S.; Mello, C.B.

doi:10.1016/j.actatropica.2015.03.021

Cited by 16 publications

(19 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In our study, all dilutions of pure C. vicina NES showed antimicrobial activity for E. coli , K. pneumoniae and C. albicans . Variations in results have been reported by various authors (Barnes et al ., 2010; Ratcliffe et al ., 2011; Ratcliffe et al ., 2015) and perhaps the reasons are abiotic factors such as diet, stress caused by laboratory conditions (temperature and humidity), NES extraction method, different diluents, or due to loss of NES activity due to bacterial contaminant LPS (lipopolysaccharides).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Antibacterial and antifungal activity of excretions and secretions of Calliphora vicina

Dallavecchia

Ricardo

Silva

et al. 2020

Medical Vet Entomology

View full text Add to dashboard Cite

Antibiotic‐resistant bacteria pose a major threat to global health in the 21st century, requiring a quick, cheap and effective response from public health officials. This study evaluated the antimicrobial activity of native excretions/secretions (NES) produced by third instar (3 days old) larvae of Calliphora vicina using a protocol adapted from the Institute of Clinical and Laboratory Standards (CLSI). The microorganisms tested were: Escherichia coli, Klebsiella pneumoniae, Staphylococcus aureus and the fungus Candida albicans. After the incubation period, the suspensions were diluted and spread on nutrient agar plates to count the colony‐forming units. A turbidimetric test also was carried out to test the action of the NES of C. vicina against S. aureus, a very common bacterial species, with an enormous capacity for adaption and resistance, being one of the bacteria of medical importance that causes the most hospital and community infections in the world. According to our results, the NES of C. vicina exhibits antimicrobial activity at different dilutions, being most effective against the gram‐negative bacteria E. coli and K. pneumoniae.

show abstract

Section: Discussionmentioning

confidence: 99%

“…Ratcliffe et al . (2015) reported reduction in E. coli growth using NES of three species of Calliphoridae ( Chrysomya megacephala , C. albiceps and C. putoria ). In contrast, Thomas et al .…”

Section: Discussionmentioning

confidence: 99%

Antibacterial and antifungal activity of excretions and secretions of Calliphora vicina

Dallavecchia

Ricardo

Silva

et al. 2020

Medical Vet Entomology

View full text Add to dashboard Cite

show abstract

“…Bexfield et al (2008) demonstrated that after 6 h, L. sericata ES was able to reduce the population of S. aureus, showing 91.5 % bactericidal potential. Jaklic et al (2008) demonstrated the inhibition of bacterial growth between 0 and 4 h and 100 % reduction of S. aureus after 8 h. Ratcliffe et al (2015) demonstrated that Chrysomya putoria Fig. 1 Growth of Pseudomonas aeruginosa colonies in CONT-BACT (bacteria control), ES+BAC (larval exosecretions with bacteria) and ES (larval exosecretions) experimental groups in seedings at times 0, 1, 2, 4, 6, and 12 h. *unc uncountable.…”

Section: Staphylococcus Aureusmentioning

confidence: 98%

First Record of Larval Secretions of Cochliomyia macellaria (Fabricius, 1775) (Diptera: Calliphoridae) Inhibiting the Growth of Staphylococcus aureus and Pseudomonas aeruginosa

et al. 2016

View full text Add to dashboard Cite

Maggot debridement therapy (MDT) consists on the intentional and controlled application of sterilized larvae of the order Diptera on necrotic skin lesions with the purpose of cleaning necrotic tissue and removing pathogenic bacteria. During MDT, a marked antimicrobial activity has been reported in literature specially associated with antibacterial substances from Lucilia sericata (Meigen); however, regarding Cochliomyia macellaria (Fabricius), little is known. This study aimed to evaluate in vitro inhibition of bacterial growth of Pseudomonas aeruginosa and Staphylococcus aureus in contact with excretions and secretions (ES) from C. macellaria larvae. Larval ES were extracted in sterile distilled water and divided in three groups: ES, containing 400 μL of autoclaved ES; ES+BAC, containing 400 μL of autoclaved ES+0.5-μL bacterial inoculum; and CONT-BAC, containing 400 μL of sterile distilled water +0.5 μL of bacterial inoculum. Aliquots of each experimental group were plated by spreading onto Petri dishes. Seedings were made at 0, 1, 2, 4, and 12 h after the extraction of ES. In ES+BAC groups, inhibition of S. aureus was verified between times 1 and 2 h and P. aeruginosa was inhibited between 0 and 4 h. There was no growth observed in any ES group. In the CONT-BAC groups, the number of colonies from time 4 h became countless for S. aureus and decreased for P. aeruginosa. As reported in the literature, we note here that ES have excellent bactericidal activity for both gram-positive and gram-negative bacteria, and this study shows for the first time the action of the bactericidal activity of exosecretions of C. macellaria against S. aureus and P. aeruginosa.

show abstract

“…To date, few studies on antibacterial activity have been reported in the haemolymph and secretion from the larvae and pupae of C. megacephala (Chaiwong et al, ; Faraldo, et al, ; Mohamed, , ; Ratcliffe et al, ; Sahalan, Omar, Mohamed, & Jeffery, ). No attempt has been made to isolate and purify antibacterial molecules with lytic properties from C. megacephala .…”

Section: Introductionmentioning

confidence: 99%

A serine protease‐associated lectin in the cytolytic system of blowfly (Chrysomya megacephala) larvae: Evidence and characterization

Paulchamy

Bhuvaragavan

Hilda

et al. 2019

Arch Insect Biochem Physiol

View full text Add to dashboard Cite

Cytolytic activity against invading microorganisms is one of the innate forms of immunity in invertebrates. A serine proteaseassociated sialic acid-specific cytolytic lectin was purified using glutaraldehyde-fixed ox erythrocytes from the larval extract of blowfly (Chrysomya megacephala). The purified lectin lysed vertebrate erythrocytes with effective haemolysis of ox red blood cells (RBCs) in an isotonic medium. The degree of haemolytic (HL) activity of the purified cytolytic lectin depended on its concentration, pH, temperature, and calcium ions. It was sensitive to ethylenediaminetetraacetic acid. The native molecular mass of the C-type lectin was 260 ± 26 kDa, comprising four different polypeptide subunits of 75 kDa (pI~8), 69 kDa (pI~7.0), 61 kDa (pI~5.3), and 55 kDa (pI~4.6). The association between the C-type lectin and serine protease was confirmed by MALDI-TOF-MS analysis that revealed its homology in the same spectral peak as well as the proteases and phenylmethylsulphonyl fluoride inhibition of HL activity. Haemolysis inhibition by N-acetylneuraminic acid and other sugars revealed the properties of the lectin. The purified lectin distorted the integrity of ox RBCs and Paenalcaligenes hermetiae. This in vitro study documents the presence of a cytolytic system in blowfly (C. megacephala) larvae for the clearance of invading microbial pathogens in their feeding niche.

show abstract

Detection and preliminary physico-chemical properties of antimicrobial components in the native excretions/secretions of three species of Chrysomya (Diptera, Calliphoridae) in Brazil

Cited by 16 publications

References 44 publications

Antibacterial and antifungal activity of excretions and secretions of Calliphora vicina

Antibacterial and antifungal activity of excretions and secretions of Calliphora vicina

First Record of Larval Secretions of Cochliomyia macellaria (Fabricius, 1775) (Diptera: Calliphoridae) Inhibiting the Growth of Staphylococcus aureus and Pseudomonas aeruginosa

A serine protease‐associated lectin in the cytolytic system of blowfly (Chrysomya megacephala) larvae: Evidence and characterization

Contact Info

Product

Resources

About