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Micropropagation of banana by means of different morphogenetic routes is an important source of genetically uniform, pest-and disease-free planting materials of Musa spp. Morphogenesis of in vitro grown plants are affected by light quality, light quantity and photoperiod. Light-emitting diode (LED) lighting systems have several unique advantages, including their peak spectral output, which closely coincides with the red absorption peak of chlorophyll and the reported wavelengths for maximum photosynthetic efficiency. The present study was aimed to investigate the effect of two LED lighting treatments (white LED and deep red/white LED) compared to conventional fluorescent lamps on the stomata formation and chlorophyll (Chl) levels in micropropagated banana plantlets through organogenesis. Both LED lighting evaluated increased the levels of total Chl, Chl-a and Chl-b in banana in vitro plantlets, indicating no statistical difference between them, but being higher then plantlets subjected to fluorescent lamps. The number of stomata was also increased equally by both LED lighting treatments, indicating increased formation of stomata in both abaxial and adaxial leaf surfaces when compared to fluorescent lamps. Although all treatments presented 100 % survival in the acclimatization phase, LED lighting show promising for micropropagation of banana due to its known superior light quality, low energy consumed, little heat generated and long operating lifetime.

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