Induction and scale-up of Billbergia zebrina nodule cluster cultures: Implications for mass propagation, improvement and conservation

Vesco, Lírio Luiz Dal; Stefenon, Valdir Marcos; Welter, Leocir José; Scherer, Ramon Felipe; Guerra, Miguel Pedro

doi:10.1016/j.scienta.2011.02.018

Cited by 22 publications

(17 citation statements)

References 27 publications

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“…The supplementation of GA 3 to MSB also provided more stability in the number of shoots among the classes of shoot height, resulting in more synchronous shoot development. In cultures of bromeliad species based on NC, both shoot regeneration and synchronism in shoot development were positively affected by the presence of GA 3 in the culture medium , Dal Vesco et al 2011, Scherer et al 2013, and the same was reported in other cultures (Dronne et al 1999, Peña-Ramírez et al 2010). …”

Section: In Vitro Phase: Shoot Regenerationsupporting

confidence: 58%

“…However, micropropagated plantlets are more expensive as compared to those produced by the conventional method based on the use of tops, slips and suckers (Teixeira et al 2001, Be andDebergh 2006). Recently, new micropropagation protocols based on nodular cluster cultures (NC) demonstrated high regenerative efficiency in Bromeliaceae species , such as Vrisea reitzii , Billbergia zebrina (Dal Vesco et al 2011), and pineapple (Scherer et al 2013). Nodular cluster cultures are globular organogenic nodule groups composed of meristematic clusters of yellow-greenish to translucent coloration, with friable to slightly compact texture .…”

Section: Introductionmentioning

confidence: 99%

“…They are involved in different plant biological processes, such as germination, reproduction, and cell elongation, among others (Hedden and Thomas 2012, Girek et al 2013, Rastogi et al 2013. Bromeliad micropropagation protocols based on organogenesis usually employ GA 3 to promote shoot elongation (Escalona et al 1999, Dal Vesco et al 2011, Scherer et al 2013. However, only a few reports have described the effects of this compound on pineapple micropropagation.…”

Section: Introductionmentioning

confidence: 99%

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Effects of immersion system and gibberellic acid on the growth and acclimatization of micropropagated pineapple

Scherer

Holderbaum

Garcia

et al. 2015

Crop Breed. Appl. Biotechnol.

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Section: In Vitro Phase: Shoot Regenerationsupporting

confidence: 58%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Effects of immersion system and gibberellic acid on the growth and acclimatization of micropropagated pineapple

Scherer

Holderbaum

Garcia

et al. 2015

Crop Breed. Appl. Biotechnol.

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“…As far as bromeliads are concerned, this biotechnology method provides an efficient commercial tool for the reintroduction of the plants, a fact which is extremely important for endangered species (Dal Vesco et al, 2011). In the last two decades, several studies regarding bromeliads have been published (Silveira et al, 2009;Santa-Rosa et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Proline levels, oxidative metabolism and photosynthetic pigments during in vitro growth and acclimatization of Pitcairnia encholirioides L.B. Sm. (Bromeliaceae)

et al. 2016

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This study aimed to evaluate the variation in the levels of proline, oxidative metabolism and photosynthetic pigments in plants of Pitcairnia encholirioides grown in vitro under different conditions and after acclimatization. The analyses were performed after 150 days of in vitro cultivation in MS media supplemented with 10 µM GA 3 or 0.2 µM NAA, sucrose at 15 or 30 g L -1 , in test tubes which allowed gas exchange or in a hermetically sealed system, and 180 days after acclimatization. The in vitro maintenance in hermetically sealed flasks, with GA 3 and 15 g L -1 sucrose had adverse metabolic effects, which was demonstrated by the lower proline and photosynthetic pigments accumulation and by the increase in antioxidant enzymes activities. After acclimatization, differences for proline and photosynthetic pigments were no longer found and the enzymatic activities ranged unevenly. The results suggest that the in vitro cultivation in media with 0.2 µM NAA and 30 g L -1 sucrose, in test tubes capped with closures which allowed gas exchange, is more suitable for micropropagation of P. encholirioides, providing a prolonged maintenance of in vitro cultures and plantlets with superior quality for ex vitro development.Keywords: enzymatic activity, oxidative stress, micropropagation, proline. Niveis de prolina, do metabolismo oxidativo e dos pigmentos fotossintéticos durante o crescimento in vitro e aclimatização de Pitcairnia encholirioides L.B. Sm. (Bromeliaceae) ResumoEste trabalho objetivou avaliar a contribuição da prolina, do metabolismo oxidativo e dos pigmentos fotossintéticos na propagação in vitro e aclimatização de Pitcairnia encholirioides, uma bromélia criticamente ameaçada de extinção. As análises foram realizadas após 150 dias de cultivo in vitro em meio MS suplementado com 10 µM de GA 3 ou 0,2 µM de ANA, 15 ou 30 g L -1 de sacarose, em tubos de ensaio que permitiam trocas gasosas ou em sistema hermeticamente vedado, e também 180 dias após aclimatização. A manutenção in vitro em frascos hermeticamente fechados, com GA 3 e 15 g L -1 de sacarose apresentou efeito metabólico adverso, demonstrado pelo menor acúmulo de prolina e pigmentos fotossintéticos e também pelo aumento das atividades de enzimas antioxidantes. Após aclimatização, as diferenças para prolina e pigmentos fotossintéticos não foram mais encontradas e as atividades enzimáticas variaram de maneira desuniforme. Os resultados sugerem que o cultivo in vitro em meio com 0,2 µM de ANA e 30 g L -1 de sacarose, em tubos fechados com tampas que permitem trocas gasosas, é mais adequado para a micropropagação de P. encholirioides, proporcionando uma manutenção prolongada das culturas in vitro e plântulas com qualidade superior para o desenvolvimento ex vitro.Palavras-chave: atividade enzimática, estresse oxidativo, micropropagação, prolina.

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“…S1, available online only at http://journals. DNA was extracted from 36 samples of NCs randomly collected among 12 treatments of micro-propagation experiments (Dal Vesco et al, 2011), comprising three samples per treatment. Aiming to evaluate the polymorphism of amplified fragment length polymorphism (AFLP) markers to assess the genetic diversity and to establish conservation strategies for this species, nodular cultures (NCs) and adult plants were genotyped and their relationship evaluated.…”

Section: Plant Materialsmentioning

confidence: 99%

Conservation of Billbergia zebrina genetic resources: AFLP polymorphism of in vitro regenerated genotypes

et al. 2011

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Biotechnological techniques comprise useful tools for the conservation of endangered plant genetic resources. In the present work, polymorphism and usefulness of amplified fragment length polymorphism (AFLP) markers in assessing the genetic diversity in populations of Billbergia zebrina were investigated in nodular cultures and adult plants of the species. AFLP markers revealed moderate-to-high genetic diversity based on the estimations of Nei's gene diversity (mean H e ¼ 0.28), Shannon index of diversity (mean H S ¼ 0.48) and the number of polymorphic fragments (mean of 56.17 polymorphic fragments over six primer pairs). In comparison to published studies of population genetics performed in other bromeliad species, the present study suggests that natural populations of B. zebrina likely maintain high levels of genetic diversity, an important feature towards conservation of plant genetic resources. The results obtained reveal that AFLP markers comprise a powerful tool in order to assess the levels of genetic diversity in natural populations of this endangered species. Integrating AFLP markers with in vitro propagation techniques is understood as an adequate strategy for conservation programmes of this species.

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Induction and scale-up of Billbergia zebrina nodule cluster cultures: Implications for mass propagation, improvement and conservation

Cited by 22 publications

References 27 publications

Effects of immersion system and gibberellic acid on the growth and acclimatization of micropropagated pineapple

Effects of immersion system and gibberellic acid on the growth and acclimatization of micropropagated pineapple

Proline levels, oxidative metabolism and photosynthetic pigments during in vitro growth and acclimatization of Pitcairnia encholirioides L.B. Sm. (Bromeliaceae)

Conservation of Billbergia zebrina genetic resources: AFLP polymorphism of in vitro regenerated genotypes

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