Theranostic nanoparticles with multifunctional ability have been emerging as a new platform for biomedical applications such as imaging, sensing and drug delivery. Despite gold nanorods (Au NRs) being an excellent nanosource with multifunctional versatility, they have certain limitations in biomedical applications, which include surfactant toxicity, biological stability and controlled drug release kinetics. Herein, we have developed Au NR-doxorubicin conjugates (DOX@PSS-Au NR) with improved drug loading efficiency (55 AE 6%) and minimum CTAB toxicity, by employing Au NRs (4.4 AE 0.5 aspect ratio) coated with poly(sodium 4-styrenesulfonate) (PSS). DOX@PSS-Au NR conjugates exhibited higher biological stability with sustained drug release kinetics at pH 5. The binding events of DOX molecules onto the PSS coated gold nanorods (PSS-Au NRs) were monitored through fluorescence quenching and the longitudinal surface plasmon resonance signals. Furthermore the anti-cancer potential and apoptosis inducing efficiency of DOX@PSS-Au NR conjugates in MCF-7 cells revealed higher therapeutic efficiency than free DOX, as corroborated through morphological assessment and in vitro cytotoxicity assay. In addition, DOX@PSS-Au NR conjugates showed efficient cellular entry and uniform intracellular distribution, suggesting the augmenting effect of chemotherapeutic drugs by Au NRs. Thus DOX@PSS-Au NR conjugates demonstrate significant therapeutic potential, suggesting their potential in anticancer therapy.
Herein,
we report facile theranostic platinum nanoparticles (PtNPs)
conjugated to an anticancer drug, doxorubicin (DOX), in unraveling
the inhibition of a cell survival PI3K/AKT (phosphatidylinositol 3-kinase/protein
kinase B) signaling pathway in MCF-7 and MDA-MB-231 human breast cancer
cells. The significant features of our DOX@PtNPs as a theranostic
platform are as follows: (i) drug release studies showed a progressive
pH-dependent delivery; (ii) in vitro studies of DOX@PtNPs
displayed a relatively higher cytotoxicity to breast cancer cells
compared to unconjugated PtNPs and DOX; (iii) intracellular drug release
studies showed a specific binding of DOX@PtNPs and their release within
the cytoplasm and perinuclear region; (iv) DOX@PtNPs induced the apoptosis
of cancer cells by DNA damage via the generation
of elevated levels of reactive oxygen species and decreased mitochondrial
membrane potential (ΔΨm), as evidenced by fluorescence
microscopic studies; and (v) DOX@PtNPs inhibited the PI3K/AKT signaling
pathway in breast cancer cells by activating PTEN, a tumor suppressor
gene. The induced mitochondrial-dependent apoptotic pathway led to
the activation of downstream caspases. Finally, our findings illustrate
that DOX@PtNPs may serve as a better theranostic agent for cancer
nanomedicine.
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