Doxorubicin-Conjugated Platinum Theranostic Nanoparticles Induce Apoptosis <i>via</i> Inhibition of a Cell Survival (PI3K/AKT) Signaling Pathway in Human Breast Cancer Cells

Patel, Parimal; Nadar, Vinita Manimaran; Umapathy, Devan; Manivannan, Selvambigai; Venkatesan, Rajiu; Arokiyam, Velanganni Antony Joseph; Srinivasan, Pappu; Prakash, P.; Jabir, Mohamed Sultan Mohamed; Gulyás, Balázs; Selvan, Subramanian Tamil; Kumar, Ponnuchamy

doi:10.1021/acsanm.0c02521

Cited by 19 publications

(29 citation statements)

References 69 publications

(121 reference statements)

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“…MDA-MB-231 triple-negative breast cancer cells were purchased from the National Centre for Cell Science, Pune, India. The cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with foetal bovine serum ( φ (FBS)=10%) and 1% ( m / V ) antibiotics (penicillin/streptomycin) at 37 °C in a humidified atmosphere with 5% CO 2 and 95% oxygen ( 17 ).…”

Section: Methodsmentioning

confidence: 99%

Capsanthin-Loaded Micelles: Preparation, Characterization and in vitro Evaluation of Cytotoxicity Using MDA-MB-231 Breast Cancer Cell Line

Shanmugham

Subban

2022

Food Technol. Biotechnol. (Online)

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Research background. Breast cancer is one of the most common cancers and remains a major cause of morbidity and mortality among women worldwide. In developed nations, breast cancer as a multifactorial disease is a major health concern, and its incidence is constantly rising in low and middle-income countries. Numerous studies have demonstrated that phytochemicals such as carotenoids inhibit breast cancer growth and induce apoptosis. We recently enhanced the aqueous solubility of capsanthin by encapsulating in diosgenin polyethylene glycol succinate, a novel non-ionic surfactant. Thus, this study aims to evaluate the cytotoxicity of aqueous soluble capsanthin-loaded micelles in MDA-MB-231 cells in vitro through MTT assay. Experimental approach. In the current study, capsanthin, a hydrophobic carotenoid, is extracted from the fruits of Capsicum annuum. Capsanthin-loaded diosgenin polyethylene glycol succinate-1000 (Cap-DPGS-1000) micelles were prepared from capsanthin extract (CAP) and diosgenin polyethylene glycol succinate 1000 (DPGS-1000) using the solid dispersion method. The capsanthin extract and Cap-DPGS-1000 were characterized by UV–visible spectroscopy, high-performance liquid chromatography (HPLC), Fourier-transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), particle size distribution, polydispersity, and scanning electron microscopy (SEM). The cytotoxicity of CAP and Cap-DPGS-1000 on a human triple-negative breast cancer cell line (MDA-MB-231) was performed to check the cell viability, proliferation, and cytotoxicity effects. Results and conclusion. The aqueous solubility of encapsulated Cap-DPGS-1000 is greatly enhanced and leads to an increased scope for localized drug delivery, a better delivery option for treating residual cancer tumors. The encapsulated capsanthin showed a sustained release in simulated intestinal fluid (pH=6.8). Our research proposes a sustained drug delivery system that ensures effective and controlled release to the affected site. The characterization data revealed no change in structure and functional groups in the encapsulated capsanthin. The IC50 value of the Cap-DPGS-1000 micelles against MDA-MB-231 breast cancer cells was (3.10±1.09) μg/mL, which is much lower than capsanthin extract (81.06±1.51) μg/mL. Capsanthin extract and capsanthin-loaded Cap-DPGS-1000 are promising drug candidates to induce apoptosis and increase reactive oxygen species (ROS) in cancer cells. Novelty and scientific contribution. The result shows the cytotoxic effect of capsanthin and capsanthin-loaded micelles on MDA-MB-231 cell line for the first time. Capsanthin from Capsicum annuum showed remarkable cytotoxic effect on the triple negative MDA-MB-231 cell line.

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Section: Methodsmentioning

confidence: 99%

Capsanthin-Loaded Micelles: Preparation, Characterization and in vitro Evaluation of Cytotoxicity Using MDA-MB-231 Breast Cancer Cell Line

Shanmugham

Subban

2022

Food Technol. Biotechnol. (Online)

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“…Metallic and metal oxide NPs can be produced and modified with a variety of chemical functional groups as needed. They can be conjugated with biological molecules (antibodies, nucleic acids or peptides), targeting ligands, and anticancer drugs using appropriate functionalization strategies ( Fu et al, 2020 ; Oliveira et al, 2020 ; Patel et al, 2020 ; Zeng et al, 2020 ). Herein, we summarize the preparation, characterization, and anti-cancer effects of platinum nanoparticles (PtNPs).…”

Section: Introductionmentioning

confidence: 99%

Platinum Nanoparticles in Biomedicine: Preparation, Anti-Cancer Activity, and Drug Delivery Vehicles

et al. 2022

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Cancer is the main cause of morbidity and mortality worldwide, excluding infectious disease. Because of their lack of specificity in chemotherapy agents are used for cancer treatment, these agents have severe systemic side effects, and gradually lose their therapeutic effects because most cancers become multidrug resistant. Platinum nanoparticles (PtNPs) are relatively new agents that are being tested in cancer therapy. This review covers the various methods for the preparation and physicochemical characterization of PtNPs. PtNPs have been shown to possess some intrinsic anticancer activity, probably due to their antioxidant action, which slows tumor growth. Targeting ligands can be attached to functionalized metal PtNPs to improve their tumor targeting ability. PtNPs-based therapeutic systems can enable the controlled release of drugs, to improve the efficiency and reduce the side effects of cancer therapy. Pt-based materials play a key role in clinical research. Thus, the diagnostic and medical industries are exploring the possibility of using PtNPs as a next-generation anticancer therapeutic agent. Although, biologically prepared nanomaterials exhibit high efficacy with low concentrations, several factors still need to be considered for clinical use of PtNPs such as the source of raw materials, stability, solubility, the method of production, biodistribution, accumulation, controlled release, cell-specific targeting, and toxicological issues to human beings. The development of PtNPs as an anticancer agent is one of the most valuable approaches for cancer treatment. The future of PtNPs in biomedical applications holds great promise, especially in the area of disease diagnosis, early detection, cellular and deep tissue imaging, drug/gene delivery, as well as multifunctional therapeutics.

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“…Noble metal based nanoparticles, such as palladium, platinum, gold, and silver have been studied in the field of medicine and pharmaceuticals due to their therapeutic potential. [1][2][3][4][5][6][7] In particular, platinum nanoparticles (PtNPs) have been reported with promising anticancer results. [8][9][10][11][12][13][14] There is evidence of PtNPs' capacity to enter the cell and damage DNA by releasing platinum ions.…”

Section: Introductionmentioning

confidence: 99%

Poly(lactic-co-glycolic acid) encapsulated platinum nanoparticles for cancer treatment

2022

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Doxorubicin-Conjugated Platinum Theranostic Nanoparticles Induce Apoptosis via Inhibition of a Cell Survival (PI3K/AKT) Signaling Pathway in Human Breast Cancer Cells

Cited by 19 publications

References 69 publications

Capsanthin-Loaded Micelles: Preparation, Characterization and in vitro Evaluation of Cytotoxicity Using MDA-MB-231 Breast Cancer Cell Line

Capsanthin-Loaded Micelles: Preparation, Characterization and in vitro Evaluation of Cytotoxicity Using MDA-MB-231 Breast Cancer Cell Line

Platinum Nanoparticles in Biomedicine: Preparation, Anti-Cancer Activity, and Drug Delivery Vehicles

Poly(lactic-co-glycolic acid) encapsulated platinum nanoparticles for cancer treatment

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