Introduction:The assessment of micronuclei in exfoliated cells is a promising tool for the study of epithelial carcinogens and can be used to detect chromosome breakage or mitotic interference, thought to be relevant to carcinogenesis.Aim:The present study aimed to detect micronuclei in exfoliated oral mucosal cells in individuals using various tobacco forms from the last 5 years.Materials and Methods:A total of 75 healthy male subjects (25 smokeless tobacco users, 25 smokers, and 25 non–tobacco users) were selected for the study. Smears were obtained with moistened wooden spatula from buccal mucosa and fixed with 95% alcohol. All the cytologic smears were stained by Papanicolaou technique. From each slide, ~1000 cells were examined under the 400× magnification and where micronucleated (MN) cells were located, they were examined under the 1000× magnification.Result:MN cells were found to be significantly higher in smokeless tobacco users than in smokers and controls.Conclusion:A positive correlation is found between increased micronucleus frequency and tobacco-using habits. So micronucleus assay can be used as a biomarker of genotoxicity.

Background:Lip prints are unique to each individual and can be used for personal identification. The lip prints show differences according to the race and the ethnic origins of a person.Aim:The objective of the study was to determine the predominant lip print type in a Punjabi population.Materials and Methods:The study was conducted on 106 students of our institution and comprised 56 males and 50 females in the age group of 18-25 years. The middle part of the lower lip was taken as study area. Analysis of lip print pattern was done according to Tsuchihashi Classification.Results:No two lip prints matched with each other. Type 1 pattern (vertical grooves that run across the entire lip) was found to be predominant in both males (51.02%) and females (43.47%) and there was no statistically observed difference between males and females in individual lip print types.Conclusion:In our study, lip prints did not match with each other, hence lip prints are unique and further studies with larger samples are required to obtain statistical significance.

Background: Extracted human teeth are regularly used for practice and educational purposes in dental institutions at undergraduate and postgraduate levels. Different materials and methods are used for sterilizing extracted teeth to avoid infection from them.

It may be concluded that chronic periodontitis and ED are associated with each other. However, further large scale studies with confounder analysis and longitudinal follow-up are warranted to explore the link between these two diseases.

Focal cemento-osseous dysplasia (FCOD) is a benign fibroosseous condition that can be seen in dentulous and edentulous patients. It is an asymptomatic lesion and needs no treatment; however, follow-up is essential due to the possibility that it can progress to a condition called florid cemento-osseous dysplasia. We report a case of FCOD of mandible in a 25-year-old female. Clinically, the lesion resembled periapical pathosis of odontogenic origin. An attempt has been made to discuss the clinical and histopathologic features along with differential diagnosis of cemento-osseous dysplasia.

Adenomatoid odontogenic tumor (AOT) is a slow-growing, benign tumor that appears in the anterior portion of the jaws and more frequently, the anterior maxilla usually in association with the crowns of unerupted teeth. Most of the tumors are diagnosed in second decade of life. A rare case report of AOT associated with an impacted right mandibular first premolar in a 24-year-old female is reported.

Riga-Fede disease (RFD) was initially described as a benign ulceration of the lingual frenum or tongue of infants, most commonly resulting from repetitive trauma by natal teeth. However, similar clinical manifestations can also be found in children with repetitive tongue-thrusting habits or certain neurological disorders. This report describes an unusual case of RFD in a 6-year-old Chinese boy. The lesion appeared as an ulcer on the anterior ventral surface of the child's tongue, and was caused by a repetitive tongue-thrusting habit. After eliminating the source of repetitive trauma and starting concurrent use of therapeutic drugs for 1 week, the lesion healed completely.

Introduction:Oral squamous cell carcinoma (OSCC) comprises one of the largest subsets of cancers with a tendency for regional metastasis. Nodal status is a key prognostic indicator in patients with OSCC, particularly with N0 neck. Occult metastasis in the form of micrometastasis (MM) and isolated tumor cells (ITCs), often goes undetected by routine hematoxylin and eosin (H&E) examination using 1–2 sections for analysis. This limitation could be overcome by combining serial sectioning (SS) with immunohistochemistry (IHC) for the detection of MM and ITC. Pan-cytokeratin (pan-CK) (AE1/AE3) is particularly a useful marker to detect these deposits as their presence has resulted in varied interpretations and different applications of the tumor-node-metastasis system.Objectives:The objective of the study was to identify a suitable method for detecting MM and ITC in lymph nodes (LNs) of OSCC by combining SS and IHC and to compare it with conventional H&E staining.Materials and Methods:This laboratory-based, prospective study was conducted on 133 LNs harnessed from ten patients treated with radical neck dissection for primary OSCC. The LNs were subjected to SS at 100 μm intervals. The sections were stained with routine H&E staining, pan-CK and analyzed for MM and ITC according to criteria laid by Hermanek et al.Statistical Analysis:The obtained data were subjected to statistical analysis using Chi-square test.Results:The application of combination of SS and IHC using pan-CK (AE1/AE3) in our study revealed the presence of MM and ITC in 2.25% of the LNs diagnosed as negative on routine H&E examination. The detection of these occult metastatic deposits resulted in upstaging of 33.33% of the patients.Conclusion:In the view of crucial role of occult LN metastasis in prognosis and survival of OSCC patients with N0 neck, diagnostic tools such as IHC staining, particularly with pan-CK (AE1/AE3), combined with SS should be preferred over conventional methods as they result in upstaging, thus sparing the low-risk patients the morbidity of unnecessary treatment.