Sterilization of extracted human teeth: A comparative analysis

Sandhu, Simarpreet V; Tiwari, Rajiv; Bhullar, Ramanpreet Kaur; Bansal, Himanta; Bhandari, Rajat; Kakkar, Tushaar; Bhusri, Ridhima

doi:10.1016/j.jobcr.2012.09.002

Cited by 43 publications

(42 citation statements)

References 23 publications

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“…The results showed that preserving extracted teeth in a 10% formalin solution for 1 week or autoclave sterilization are the best antimicrobial methods (Table 2). 10,11 Similar conclusions were reached by Sandhu et al 10 when preserving samples for 5 days. In the first stage of their experiment, different sterilizing agents were used; the samples were then incubated on agar medium.…”

Section: Sterilization Of Extracted Human Teethsupporting

confidence: 72%

“…Steam autoclave [10][11][12][13][14]17,25 Ethanol 21,23 YAG laser 16,29 NaOCl 10,11,17,19,21 Microwaves 20,21 Hydrogen peroxide 10,15,18,21,23 Gamma radiation 16,27 Thymol 10…”

Section: Solution Methodsmentioning

confidence: 99%

“…9 In order to ensure epidemiological safety, materials must be sterilized or disinfected before use. 10,11 The aim of disinfection is to kill pathogens by means of chemical solutions or miscellaneous inactivating agents. 12 Sterilization is a process that eradicates not only all living forms of microorganisms but their heatresistant vegetable forms (bacterial spores) as well.…”

Section: Sterilization Of Extracted Human Teethmentioning

confidence: 99%

“…21,23 Table 1. The most frequent methods tested for sterilizing/disinfecting extracted human teeth [10][11][12][13][14][15][16][17][18][19][20][21]23,25,27,29,30 Non-solution methods…”

Section: Sterilization Of Extracted Human Teethmentioning

confidence: 99%

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Extracted human teeth and their utility in dental research. Recommendations on proper preservation: A literature review

Nawrocka¹,

Łukomska−Szymańska²

2019

Dent. Med. Probl.

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Laboratory research in dentistry and dental education use extracted human teeth as a model for simulation for ex vivo procedures. Human-borne tissues are the first choice of material for bond strength assessment. To obtain comparable results in dental material tests and to ensure microbiological safety, specimens must be stored under specific, uniform conditions. The aim of this paper was to present the contemporary view and recommendations on preserving extracted human teeth. The antimicrobial properties of the storage medium are a crucial aspect, as extracted teeth pose a risk of cross-infection. A classification of different methods (using solutions and otherwise) is presented and their sterilizing efficiency is compared based on the literature. The emphasis is put on the interaction between the storage conditions and the substrate. Tooth specimens should be biologically safe and have normal mechanical properties. The sterilizing process must be neutral for the enamel and dentin microstructure, because even a minor change can affect the adhesive bonding. Autoclave sterilization and storage in 10% formalin solution are widespread and reliable methods, although they do have their disadvantages. There is a need for further investigation in order to establish uniform recommendations on preparing and preserving extracted human teeth used for research purposes.

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Section: Sterilization Of Extracted Human Teethsupporting

confidence: 72%

“…Steam autoclave [10][11][12][13][14]17,25 Ethanol 21,23 YAG laser 16,29 NaOCl 10,11,17,19,21 Microwaves 20,21 Hydrogen peroxide 10,15,18,21,23 Gamma radiation 16,27 Thymol 10…”

Section: Solution Methodsmentioning

confidence: 99%

Section: Sterilization Of Extracted Human Teethmentioning

confidence: 99%

“…21,23 Table 1. The most frequent methods tested for sterilizing/disinfecting extracted human teeth [10][11][12][13][14][15][16][17][18][19][20][21]23,25,27,29,30 Non-solution methods…”

Section: Sterilization Of Extracted Human Teethmentioning

confidence: 99%

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Extracted human teeth and their utility in dental research. Recommendations on proper preservation: A literature review

Nawrocka¹,

Łukomska−Szymańska²

2019

Dent. Med. Probl.

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“…The former teeth were donated from the tooth bank of the School of Dentistry of Universidade Federal Fluminense (UFF), Brazil. The teeth, after extraction, were conserved following a procedure established in the literature [24,25]. Essentially, they were cleaned, sterilized in an autoclave and then kept immersed in cold distilled water until their use.…”

Section: Methodsmentioning

confidence: 99%

Evaluation of polymerization shrinkage of dental composites by an optical method

Weig

Filho

Neto

et al. 2015

Materials Science and Engineering: C

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Human teeth biobank: Microbiological analysis of the teeth storage solution

Curylofo-Zotti

Lorencetti-Silva

Coelho

et al. 2018

Microscopy Res & Technique

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The cross-infections may occur during handling of dental elements, affecting the health of dental practitioners and researchers. This study aimed to analyze the influence of the storage medium temperature on the bacterial contingent of the human teeth used for research purposes. Thirty human teeth were donated to the Human Teeth Biobank immediately after extraction. The teeth were cleaned with tap water and neutral soap. The teeth were randomly distributed according to the temperature of the storage solution (deionized water): at 4 °C (refrigerator) or at -10 °C (freezer) and were stored individually in sterile vials during 60 days. After this period, a microbiological analysis (CFU/mL) of the storage solutions was performed and teeth were submitted to SEM analysis. Data were analyzed by Kruskal-Wallis test followed by Dunn's post-test (p ≤ .05). Total aerobic bacteria ranged from 5.8 to 8.4 log CFU/mL for refrigerated solution and from 1.9 to 8.5 log CFU/mL for frozen solution. No statistical differences were found between the storage solutions (p > .05). The counts of Streptococcus spp., Lactobacillus spp., and Staphylococcus spp. were similar for both storage solutions (p > .05). SEM analysis showed spiral- and rod-shaped bacteria attached on teeth stored under 4 °C, which may suggest the presence of Treponema spp. and Lactobacillus spp. Similar morphological forms were found on teeth stored under -10 °C. A biofilm organized in honeycomb-like form was found in the frozen teeth. Cocci were eventually found in all the samples. It was concluded that bacterial growth and survival were not influenced by the temperature of the teeth storage solution.

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Sterilization of extracted human teeth: A comparative analysis

Abstract: Background: Extracted human teeth are regularly used for practice and educational purposes in dental institutions at undergraduate and postgraduate levels. Different materials and methods are used for sterilizing extracted teeth to avoid infection from them.

Cited by 43 publications

References 23 publications

Extracted human teeth and their utility in dental research. Recommendations on proper preservation: A literature review

Extracted human teeth and their utility in dental research. Recommendations on proper preservation: A literature review

Evaluation of polymerization shrinkage of dental composites by an optical method

Human teeth biobank: Microbiological analysis of the teeth storage solution

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