Human teeth biobank: Microbiological analysis of the teeth storage solution

Abstract

The cross-infections may occur during handling of dental elements, affecting the health of dental practitioners and researchers. This study aimed to analyze the influence of the storage medium temperature on the bacterial contingent of the human teeth used for research purposes. Thirty human teeth were donated to the Human Teeth Biobank immediately after extraction. The teeth were cleaned with tap water and neutral soap. The teeth were randomly distributed according to the temperature of the storage solution (deionized water): at 4 °C (refrigerator) or at -10 °C (freezer) and were stored individually in sterile vials during 60 days. After this period, a microbiological analysis (CFU/mL) of the storage solutions was performed and teeth were submitted to SEM analysis. Data were analyzed by Kruskal-Wallis test followed by Dunn's post-test (p ≤ .05). Total aerobic bacteria ranged from 5.8 to 8.4 log CFU/mL for refrigerated solution and from 1.9 to 8.5 log CFU/mL for frozen solution. No statistical differences were found between the storage solutions (p > .05). The counts of Streptococcus spp., Lactobacillus spp., and Staphylococcus spp. were similar for both storage solutions (p > .05). SEM analysis showed spiral- and rod-shaped bacteria attached on teeth stored under 4 °C, which may suggest the presence of Treponema spp. and Lactobacillus spp. Similar morphological forms were found on teeth stored under -10 °C. A biofilm organized in honeycomb-like form was found in the frozen teeth. Cocci were eventually found in all the samples. It was concluded that bacterial growth and survival were not influenced by the temperature of the teeth storage solution.

3D-Printed Teeth in Endodontics: Why, How, Problems and Future—A Narrative Review

“…Nowadays, the biobank is considered a primary resource for the development of precision medicine. The purpose of biobanks is to support and improve clinical and biomedical research, especially for preclinical trials [38,39]. As a result, these teeth are not available for teaching purposes.…”

Biobanking in dentistry: A review

“… 4 °C (refrigerator) −10 °C (freezer) Tvinnereim et al (2012) [ 44 ] Rinse teeth in clean water, without using detergents, and leave to dry overnight before placing them in a tube. Or Curylofo-Zotti et al (2018) [ 64 ] Biobank personnel inspect the teeth. If a tooth has visible traces of blood, is still damp and/or smells, it is rinsed in distilled water and dried overnight before its examination and recording of variables Solution of distilled water alone, distilled water with thymol, 70% ethanol, 10% formalin, and phosphate-buffered saline with thymol Or Dry, at room temperature Adult teeth Extracted teeth are placed in sealed containers containing 75% alcohol.…”

“…If a tooth has visible traces of blood, is still damp and/or smells, it is rinsed in distilled water and dried overnight before its examination and recording of variables Solution of distilled water alone, distilled water with thymol, 70% ethanol, 10% formalin, and phosphate-buffered saline with thymol Or Dry, at room temperature Adult teeth Extracted teeth are placed in sealed containers containing 75% alcohol. 75% alcohol Freezer or refrigerator Variable Procure and store teeth, then process them to obtain bone graft substitutes Room temperature Pang et al (2017) [ 40 ] Adult teeth After extraction, superficial dirt is removed with tap water and neutral soup Deionized water Room temperature Immediately after extraction Research purposes 4 °C or −10 °C Curylofo-Zotti et al (2018) [ 64 ] Stem cells from dental pulp (DPSC) Extracted teeth are placed in tubes containing Dulbecco’s modified Eagle’s medium (DMEM) with 20% fetal bovine serum (FBS). Dulbecco’s modified Eagle’s medium (DMEM) with 20% fetal bovine serum (FBS).…”

Obtaining human teeth for dental education: A cross‐sectional study to create ethical and transparent processes

“…[14][15][16] The use of human teeth, however, has drawbacks since they are considered dangerous sources of crosscontamination requiring infection control protocols. 17,18 In addition, ethical guidelines must be established regarding human tooth origin and acquisition. Historically teeth have been obtained from dental offices without any official record or informed consent.…”

“…The use of human teeth, however, has drawbacks since they are considered dangerous sources of cross‐contamination requiring infection control protocols 17,18 . In addition, ethical guidelines must be established regarding human tooth origin and acquisition.…”