Anionic phospholipids are essential structural components of cell membranes. Spatiotemporal dynamics of these lipids play central roles in regulating signalling events, membrane trafficking, maintenance of cell‐shape, and cargo transport. On the other hand, defects in anionic phospholipid metabolism are linked to multiple diseases. Hence, the ability to visualize these phospholipids and their dynamics in living cells can afford mechanistic insights into vital cell processes, guide the development of therapeutics, and lead to diagnostic agents. In this exciting backdrop, fluorescent sensors that can detect anionic phospholipids become key chemical tools that can be used to image and track these bio‐molecules in a confocal microscopy platform. In this review, we highlight existing chemical probes and sensing strategies for anionic phospholipids along with their pros and cons in the context of their applicability toward imaging and tracking these essential lipids in living cells.
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