Precise in situ photo-induced pH modulation during NMR spectrometry

Dey, Santanu S.; Bielytskyi, Pavlo; Gräsing, Daniel; Das, Anirban; Kundu, Rajasree; Matysik, Jörg; Maiti, Sudipta; Madhu, Perunthiruthy K.

doi:10.1016/j.cplett.2018.07.009

Cited by 3 publications

(7 citation statements)

References 18 publications

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“…Acid‐base reaction is one of the fundamental chemical reactions in nature and has implications in many natural and biological processes . The dissociation equilibrium of an acid HA into proton and its deprotonated species (A − ) is,

true H A = H^{+} + A^{-}

…”

Section: Introductionmentioning

confidence: 99%

“…The primary prcocesses of excited state proton transfer (ESPT) of HPTS in a wide variety of media have been monitored by time evolution of the fluorescence from the anion (A − *) and the undissociated acid (HA*). This includes binary solvent mixtures, live biological cell, reverse micelles, micelle, nafione membrane and niosome .…”

Section: Introductionmentioning

confidence: 99%

“…[8][9][10][11][12] ESPT is implicated in rhodopsin (involved in the vison process) [13] and in green fluorescence protein (GFP). [14][15] The primary prcocesses of excited state proton transfer (ESPT) of HPTS in a wide variety of media [10][11][12][16][17][18][19][20][21][22][23][24][25][26][27][28][29][30][31] have been monitored by time evolution of the fluorescence from the anion (A À *) and the undissociated acid (HA*). This includes binary solvent mixtures, [10][11]22] live biological cell, [17,18] reverse micelles, [19,30,32] micelle, [21] nafione membrane [30] and niosome.…”

Section: Introductionmentioning

confidence: 99%

“…[29] ESPT of HPTS has not been studied in polymer hydrogel. In spite of the great deal of work on HPTS, [10][11][12][16][17][18][19][20][21][22][23][24][25][26][27][28][29][30][31] there has been almost no effort to understand how the local pH around a photo-acid varies with time, following excitation of the photoacd. Huppert and co-workers proposed that rise time of the A À emission scales with pK a * in the case of 2-naphthols and using this they calculated pK a * of pyranine (8-hydroxypyrene-1,3,6trisulphonic acid, HPTS, Scheme 1).…”

Section: Introductionmentioning

confidence: 99%

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Time Evolution of Local pH Around a Photo‐Acid in Water and a Polymer Hydrogel: Time Resolved Fluorescence Spectroscopy of Pyranine

et al. 2019

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In this work, we propose a new analysis of the time resolved emission spectra of a photo‐acid, HA, pyranine (8‐hydroxypyrene‐1,3,6‐trisulphonic acid, HPTS) based on time resolved area normalized emission spectra (TRANES). Presence of an isoemissive point in TRANES confirms the presence of two emissive species (HA and A−) inside the system in bulk water and inside a co‐polymer hydrogel [F127, (PEO)100–(PPO)70–(PEO)100]. We show that following electronic excitation, the local pH around HPTS, is much lower than the bulk pH presumably because of ejection of proton from the photo‐acid in the excited state. With increase in time, the local pH increases and reaches the bulk value. We further, demonstrate that the excited state pKa of HPTS may be estimated from the emission intensities of HA and A− at long time. The time constant for time evolution of pH is ∼630 ps in water, ∼1300 ps in F127 gel and ∼4700 ps in CTAB micelle. The location and local viscosity sensed by the probe is ascertained using fluorescence correlation spectroscopy (FCS) and fluorescence anisotropy decay. The different values of the local viscosity reported by these two methods are reconciled.

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true H A = H^{+} + A^{-}

…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Time Evolution of Local pH Around a Photo‐Acid in Water and a Polymer Hydrogel: Time Resolved Fluorescence Spectroscopy of Pyranine

et al. 2019

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“…Some of the more recently developed tools focus on various sources of cue-dependent pH manipulations. NMR instruments have been shown to be capable of inducing pH changes in solution [ 120 ]. Some examples include small molecule photoacids, which release a proton upon photo-uncaging with UV light [ 121 ].…”

Section: Tools To Manipulate Phimentioning

confidence: 99%

Cancer and pH Dynamics: Transcriptional Regulation, Proteostasis, and the Need for New Molecular Tools

Czowski

Romero-Moreno

Trull

et al. 2020

Cancers

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An emerging hallmark of cancer cells is dysregulated pH dynamics. Recent work has suggested that dysregulated intracellular pH (pHi) dynamics enable diverse cancer cellular behaviors at the population level, including cell proliferation, cell migration and metastasis, evasion of apoptosis, and metabolic adaptation. However, the molecular mechanisms driving pH-dependent cancer-associated cell behaviors are largely unknown. In this review article, we explore recent literature suggesting pHi dynamics may play a causative role in regulating or reinforcing tumorigenic transcriptional and proteostatic changes at the molecular level, and discuss outcomes on tumorigenesis and tumor heterogeneity. Most of the data we discuss are population-level analyses; lack of single-cell data is driven by a lack of tools to experimentally change pHi with spatiotemporal control. Data is also sparse on how pHi dynamics play out in complex in vivo microenvironments. To address this need, at the end of this review, we cover recent advances for live-cell pHi measurement at single-cell resolution. We also discuss the essential role for tool development in revealing mechanisms by which pHi dynamics drive tumor initiation, progression, and metastasis.

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Photoacid Generators for Biomedical Applications

Sun,

Kang,

Zhao

et al. 2023

Advanced Science

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Photoacid generators (PAGs) are compounds capable of producing hydrogen protons (H+) upon irradiation, including irreversible and reversible PAGs, which have been widely studied in photoinduced polymerization and degradation for a long time. In recent years, the applications of PAGs in the biomedical field have attracted more attention due to their promising clinical value. So, an increasing number of novel PAGs have been reported. In this review, the recent progresses of PAGs for biomedical applications is systematically summarized, including tumor treatment, antibacterial treatment, regulation of protein folding and unfolding, control of drug release and so on. Furthermore, a concept of water‐dependent reversible photoacid (W‐RPA) and its antitumor effect are highlighted. Eventually, the challenges of PAGs for clinical applications are discussed.

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Precise in situ photo-induced pH modulation during NMR spectrometry

Cited by 3 publications

References 18 publications

Time Evolution of Local pH Around a Photo‐Acid in Water and a Polymer Hydrogel: Time Resolved Fluorescence Spectroscopy of Pyranine

Time Evolution of Local pH Around a Photo‐Acid in Water and a Polymer Hydrogel: Time Resolved Fluorescence Spectroscopy of Pyranine

Cancer and pH Dynamics: Transcriptional Regulation, Proteostasis, and the Need for New Molecular Tools

Photoacid Generators for Biomedical Applications

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