The aim of this work was to investigate possible protective effect of celery juice in doxorubicin treatment. The following biochemical parameters were determined: content of reduced glutathione, activities of catalase, xanthine oxidase, glutathione peroxidase, peroxidase, and lipid peroxidation intensity in liver homogenate and blood hemolysate. We examined influence of diluted pure celery leaves and roots juices and their combinations with doxorubicine on analyzed biochemical parameters. Celery roots and leaves juices influenced the examined biochemical parameters and showed protective effects when applied with doxorubicine.

Given the widespread use of dietary supplements (DS) and herbal products (HP), healthcare professionals (HCPs) will increasingly encounter patients who use these preparations with conventional drugs and who need their services to reduce the consequences of adverse therapeutic outcomes. The aim of our survey was to assess the knowledge and behaviors of HCPs regarding the risk of potential drug–dietary supplement (DDSIs) and drug–herbal product (DHPIs) interactions. This cross-sectional survey collected data via on paper-based questionnaire among general practitioners (GPs) (n = 105), specialty doctors (n = 87) and nurses (n = 154). The HCPs were mostly familiar with the interaction of doxycycline with magnesium (83%) and were least familiar with interaction of warfarin with glucosamine (14%). The results on DDSIs and DHPIs knowledge showed that GPs scored significantly higher than nurses (p < 0.001 and p = 0.003, respectively), while specialty doctors scored significantly higher than nurses only on DDSIs knowledge (p < 0.001). Only 28% of respondents reported that they often or always ask patients on drug therapy about the use of DS or HP, and 25% of respondents record such data in the medical documentation of patients. Our results showed that HCPs have sufficient knowledge about most major DDSIs and DHPIs, but insufficient knowledge about most moderate interactions. However, their overall knowledge and behavior regarding the risk of these interactions indicate the need for further continuing education and training.

Background: The purpose of this study is to determine the effect of L-ascorbic acid and alpha-tocopherol as well as combination of these vitamins with or without exposure to physical exercise on intensity of lipid peroxidation, activity of xanthine oxidase, activity of total antioxidative system, concentration of glutathione, and activity of catalase in the serum of guinea pigs. Materials and Methods: The experimental measurements of intensity of lipid peroxidation, activity of xanthine oxidase, activity of total antioxidative system, concentration of glutathione, and activity of catalase were done in the serum of guinea pigs. The animals were exposed to the test load to achieve exhaustion and the test was terminated when the animal for the third time to sink into the water. Results:The results of this study demonstrated that endurance exercise of guinea pigs induced oxidative stress response in terms of increased lipid peroxidation and activity of xanthine oxidase in the serum of experimental animals. Our study investigated the antioxidant activity of L-ascorbic acid and alpha-tocopherol also measuring three protective markers in the serum: total antioxidant activity, content of glutathione and activity of catalase. The results obtained show that the vitamins influence the concentrations of above mentioned biochemical parameters, which points out their protective effect of swimming-induced oxidative stress. Conclusion: Single or combined administration of L-ascorbic acid and alpha-tocopherol caused significant inhibition of these markers indicating the important antioxidant activity of the vitamins. Results lead to conclude that the combined treatments with vitamins with or without exposure to physical exercise showed the clear synergistic effect..

The effects of different extracts of parsley and celery leaves (Et 2 O, CHCl 3 , EtOAC, n-BuOH, and H 2 O) on ketamine-induced sleeping time have been investigated. The experiments were conducted on BALB=C white laboratory mice divided in five groups. On the first day, each group received ketamine 40 mg=ml s.c. After 5 t 1=2 (next day), mice were treated with 10% extracts (Et 2 O, CHCl 3 , EtOAC, n-BuOH, H 2 O) of parsley and celery leaves, 1 ml=kg i.p. in two doses, each after 2-h interval. One hour after the last dose, animals received ketamine 40 mg=kg s.c. Just after administration of ketamine, induction time and time of sleeping were measured. Most of the examined extracts of both plants exhibited insignificant changes in induction time. Only the EtOAc extract of celery exhibited significant increase, whereas Et 2 O and n-BuOH extract of parsley induced significant decrease of induction time in treated animals. Furthermore, all examined extracts of both plants exhibited increase of sleeping time in animals treated with ketamine. The Et 2 O extract of both celery and parsley exhibited the highest effect. Generally, examined celery extracts caused prolongation of sleeping time in animals compared with those obtained for parsley.

Extracts of celery (Apium graveolens L.; Apiaceae) leaves prepared in solvents of different polarity, viz., ether, chloroform, ethyl acetate, n-butanol, and water, were used to study their antipyretic effect in mice. Experiments were conducted on white laboratory mice divided into five groups. On the first day of the experiment, their rectal temperatures were measured every 30 min during 5 h (basal temperature). On the second day, the animals were given 12% yeast suspension to induce a pyrogenic effect and on the third day the same dose of yeast and the appropriate celery extract, and the rectal temperatures were measured in the same way as basal temperature. By plotting the measured temperatures versus time, the corresponding areas-under-the-curve (AUC) were obtained for each group of animals. The AUC values were used to determine statistically significant differences between them. The results showed that extracts of celery leaf decreased (annuled) the pyrogenic effect of 12% yeast suspension.