Aganaspis pelleranoi (Brè thes) (Hymenoptera: Figitidae) is an important fruit ßy parasitoid native to the Neotropics. We investigated the biology and constructed a fertility life table for A. pelleranoi reared on larvae of Anastrepha fraterculus (Wiedemann) and Ceratitis capitata (Wiedemann) (Diptera: Tephritidae). Parasitoid development was investigated in larvae of the two fruit ßy species by recording the duration of the eggÐadult period, number of offspring, sex ratio, adult weight, adult hind tibia length, and male and female longevity, and constructing a fertility life table. Parasitoids reared on An. fraterculus larvae produced more offspring, had shorter eggÐadult periods, greater proportion of females and longer survival, and higher percent emergence than parasitoids reared on C. capitata larvae. Moreover, A. pelleranoi individuals reared on An. fraterculus larvae were heavier and had longer hind tibia than those reared on C. capitata. Based on life table statistics, the time required to double the population (T d) was Ϸ3.4 times shorter for parasitoids reared on An. fraterculus larvae. Our results show that A. pelleranoi develops better on larvae from An. fraterculus than C. capitata, indicating that this species could be used for the mass production of this parasitoid intended for augmentative biological control of fruit ßies.

Anastrepha fraterculus (Wiedemann) (Diptera: Tephritidae) is among the most important pests of fruit trees in South America, where it can cause losses of up to 100% in fruit orchards. The endoparasitoid Aganaspis pelleranoi (Brèthes) (Hymenoptera: Figitidae) is one of the main natural enemies of A. fraterculus in Neotropical countries and can be used as a biological control agent against this pest. This study aimed to provide background biological information for the development of a method for mass rearing of A. pelleranoi in larvae of A. fraterculus We determined the effects of diet on the longevity and parasitism capacity of A. pelleranoi adults, the optimal instar of A. fraterculus for parasitism, the ideal exposure time of the larvae to the parasitoid, and the parasitism capacity of females of A. pelleranoi The results showed that a 30% honey:water solution maximized parasitoid longevity. Third-instar larvae of A. fraterculus should be used in parasitoid multiplication. An exposure time of 4 h of A. fraterculus larvae produced a larger number of parasitoid offspring, with the highest proportion of females. In addition, the estimated mean parasitism capacity of females of A. pelleranoi was 10 larvae per day. This information can help to develop a mass-rearing method for A. pelleranoi in larvae of A. fraterculus.

Doryctobracon brasiliensis (Sz epligeti) is a parasitoid larval-pupal of fruit flies and has great potential to be used in biological control programmes as it feeds on other Anastrepha species in addition to Anastrepha fraterculus (Wiedemann). This study investigated the biology of D. brasiliensis at different temperatures to design a life fertility table and determine thermal requirements. The parasitoids were multiplied in larvae of A. fraterculus in air-conditioned chambers at 15, 18, 20, 22, 25, 28 and 30°C, 70 AE 20% RH and photophase of 12 h. We determined the number of offspring, sex ratio, longevity of males and females and duration of egg-adult period. The temperature range 18-22°C ensures higher fecundity and at 20°C, and the average number of offspring per female was 152.77 parasitoids. The sex ratio of offspring produced was reduced with increasing temperatures. Longevity of males and females of D. brasiliensis was reduced by increasing temperatures. At 15, 28 and 30°C, there was no development of immature stages. For the temperature range 18-25°C, the duration of egg-adult period of D. brasiliensis was inversely proportional to temperature. At 20 and 22°C, we observed the highest values of net reproduction rate (Ro) and finite reason of increase (k), meaning that at the estimated optimum temperature (21°C), the population of D. brasiliensis increased 47 times each generation. The lower temperature threshold for development was 10.01°C and the thermal constant (K) 303.21 degree/days. This information confirms that D. brasiliensis is better suited to temperate environments, which implies a significant potential for the use of D. brasiliensis in the control of A. fraterculus, because most areas occupied by this pest are in temperate regions. In addition, D. brasiliensis is useful in mass rearing systems in laboratory.

Aganaspis pelleranoi (Brèthes, 1924) (Hymenoptera: Figitidae) is a koinobiont endoparasitoid of larvae of species of the genus Anastrepha and of Ceratitis capitata. It is a candidate for use as a biological control agent, as under field conditions, it may reach a parasitism rate of 62%. The objective of this study was to determine the effect of different constant temperatures on biological parameters of A. pelleranoi when parasitizing the larva of Anastrepha fraterculus (Wiedemann, 1830) (Diptera: Tephritidae), as well as to determine its thermal requirements. The study was conducted in environmental chambers at 18, 20, 22, 25, 28, and 30 ± 1°C; 70 ± 10% relative humidity; and a 12-h photophase. Females maintained at 18 and 20°C produced more progeny than females at other temperatures tested. The longevity of males and females was inversely proportional to temperature, ranging from 49.1 to 3.73 d for females and from 32.1 to 3.8 d for males at temperatures of 18-30°C, respectively. The duration of the biological cycle (egg-to-adult) was influenced by temperature, and ranged from 69.1 d at 18°C to 30 d at 25°C. No preimaginal development of A. pelleranoi occurred at 28 and 30°C. The relationship between temperature and the demographic parameters of A. pelleranoi showed a linear effect over the temperature range of 18-25°C. The lower temperature threshold and thermal constant were 11.69°C and 391.70 degree days, respectively.

Fitness in parasitoids is generally influenced by host quality. We evaluated the development parameters of Doryctobracon areolatus (Hymenoptera: Braconidae) parasitoid on Anastrepha fraterculus (Diptera: Tephritidae) larvae, reared in artificial diets with different protein sources. Larvae of A. fraterculus were reared in the diets based on 1) raw wheat germ (control); 2) whole rice flour; 3) corn flour; and, 4) whole wheat flour + soybean meal. The larvae were used for the development of D. areolatus, to evaluate the number of offspring/female, emergence rate, sex ratio, egg-adult period, adult weight posterior tibia length and longevity. Larvae of A. fraterculus reared in the diets based on wheat germ and corn flour obtained a greater number of offspring, with shorter duration of egg-adult period for males and females. In diets with rice flour and corn flour, A. fraterculus larvae generated adults of D. areolatus with greater weight, longer tibia length and higher sex ratio, whereas larvae reared in wheat flour + soybean meal diet had a lower sex ratio. The diets based on rice flour and corn are the most appropriate for multiplication of the parasitoid.

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