Pour la première fois deux souches de Leishmanies, isolées en Cévennes d'une popula tion naturelle de Phlebotomus ariasi, sont identifiées par la méthode enzymatique. Elles se rap portent toutes deux au zymodème 1, caractéristique de Leishmania infantum s. st. Ecology of leishmaniasis in the south of France. 18. Enzymatic identification of Leishmania infantum Nicolle, 1908, isolated from Phlebotomus ariasi Ton noir, 1921, spontaneously infected in the Cévennes. SUMMARY. Out of 187 female Phlebotomus ariasi caught in the Cévennes focus of leishmaniasis 3 were found naturally infected with Leishmania. The infection in one of the three had spread from the midgut to the pharynx and proboscis. Stocks of Leishmania were isolated from two of the flies and 8 isoenzymes were examined. The newly isolated parasites were found to be in distinguishable from zymodeme 1 of Leishmania infantum s. st.

A double clone of Trypanosoma platydactyli Catouillard, 1909, derived from a single trypomastigote from the blood of the Moorish gecko, Tarentola mauritanica, was grown in vitro. Morphogenesis of the parasites led to a stable population of promastigotes which were identical, in general morphology, ultrastructure and the electrophoretic mobility of 8 enzymes, to those cultures previously considered to be Leishmania tarentolae Wenyon, 1921. These cultures included the strain isolated from the type locality in Algeria by Parrot (1949) and later used as a laboratory model for the genus Leishmania. T. platydactyli and L. tarentolae are synonymized and the present status of saurian Leishmania parasites is discussed.

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A nnales de Parasitologie (Paris ; 1982, t. 57, n° 6, pp. 527

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