Typage enzymatique de onze souches de <i>Leishmania</i> isolées, en Italie continentale, à partir de formes viscérales murines, canines et vulpines

Gramiccia, Marina; Maazoun, R.; Lanotte, G.; Rioux, J. A.; Blancq, S. Le; Evans, David A.; Peters, W.H.M.; Bettini, S.; Gradoni, Luigi; Pozio, Edoardo

doi:10.1051/parasite/1982576527

Cited by 30 publications

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“…Strain identification is a basic investigation useful for the knowledge of Leishmania foci and the understanding of the clinical outcome of leishmaniasis. The ecoepidemiological studies of leishmaniasis have made real progress thanks to the isoenzyme identification [20][21][22][23][24]. Identical clinical lesions may occur due to different species of Leishmania, which stresses the high interest of isoenzyme identification of the strains occurring in the area of Annaba [25].…”

Section: Discussionmentioning

confidence: 99%

“…In Tunisia, Kallel et al (2005) L. infantum MON-1 is the principal agent of Mediterranean VL [38]. However, it was isolated in cases of SCL in Algeria [3,32,39], in Tunisia [40], in France [25],in Spain [29], in Greece [31], in Italy [23] and in Portugal [41]. It has been described in Tunisia first in SCL by Aoun et al (2000), with two strains against six of L. infantum MON-24.…”

Section: Discussionmentioning

confidence: 99%

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The First Isoenzymatic Characterizations of the Leishmania Strains Responsible for Cutaneous Leishmaniasis in the Area of Annaba (Eastern Algeria)

Mansouri¹

2012

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The epidemiological situation of cutaneous leishmaniasis in the region of Annaba (North Eastern Algeria) is explored for the first time. During a clinical survey carried out in the Annaba Hospital between 2004 and 2008, the parasitological study of lesions has revealed 259 positive cases (43.31%). Isoenzymatic identification of 16 strains isolated showed the presence of three Leishmania species and four zymodemes. The hypothesis of the presence of L. infantum is confirmed with two zymodemes, MON-1 and MON-24. The unexpected presence of L. major MON-25 certifies the substantial extension of this zoonotic form to the North. The isolation of L.killicki, whose presence was unsuspected, reiterated the interest of eco-epidemiological analysis of households affected by the disease. Moreover, it is a new zymodeme never isolated so far, the MON-306. © Mansouri et al.; Licensee Bentham Open. This is an open access article licensed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

The First Isoenzymatic Characterizations of the Leishmania Strains Responsible for Cutaneous Leishmaniasis in the Area of Annaba (Eastern Algeria)

Mansouri¹

2012

TOPROCJ

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“…DNA was extracted from skin biopsy samples (PCR template preparation kit, Roche, Germany) and analysed by real-time TaqMan ' PCR for Leishmania DNA detection (Rolão et al, 2004). The PCR primers (forward, 5'-GGTTAGCCGATGGTGGTCTT-3', reverse, 5'-GCTATATCATATGTCCAAGCACTTACCT-3') and the TaqMan ® internal probe (5'-ACCACCTAAGGTCAACCC-3') (Applied Biosystems, Foster City, CA, USA) were designed from a kDNA minicircle sequence of a mediterrean region isolate of L. infantum (Genebank A/N AF169140 (Rioux et al, 1968), Italy (Gramicia et al, 1982), Portugal (Abranches et al, 1984) and Spain (Fisa et al, 1999), and black rats in Italy (Gradoni et al, 1983) and Spain (Morillas-Márquez et al, 1985), which have been considered wild reservoirs. Cats in Portugal (Durão et al, 1994), Spain (Hervas et al, 1999), France (Ozon et al, 1998) and Italy (Poli et al, 2002;Pennisi et al, 2004), and a wolf in Portugal (Rebelo, personal communication) have also been found parasitized and generally considered as accidental hosts.…”

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Equine infection withLeishmaniain Portugal

et al. 2005

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Summary :The present report describes the first case of equine leishmaniasis in Portugal. Leishmania infection was detected in one animal, which presented an ulcerated skin lesion. Diagnosis was based on serology by CIE, and parasite DNA detection by real-time PCR using a probe specific for L. infantum. This finding requests further leishmaniasis equine surveys in order to clarify the role of the horse as reservoir host in european endemic areas. Résumé MATERIAL AND RESULTS The present study was conducted within the framework of a canine epidemiological survey in the endemic Metropolitan Region of Lisbon. A serological screening was carried out in 13 horses living in a farm from the referred endemic area, where three dogs were previously diagnosed with leishmaniasis. The horses were born in Portugal and had never travelled abroad. Serological samples were analysed by counterimmunoelectrophoresis (CIE). The CIE was carried out according to the procedure of Campino et al. (1995), using cultured promastigotes of L. infantum MON-1 as antigen. Sera samples were used undiluted and all the reactions with at least one precipitation arc were considered positive. DNA was extracted from skin biopsy samples (PCR template preparation kit, Roche, Germany) and analysed by real-time TaqMan ' PCR for Leishmania DNA detection (Rolão et al., 2004). The PCR primers (forward, 5'-GGTTAGCCGATGGTGGTCTT-3', reverse, 5'-GCTATATCATATGTCCAAGCACTTACCT-3') and the TaqMan ® internal probe (5'-ACCACCTAAGGTCAACCC-3') (Applied Biosystems, Foster City, CA, USA) were designed from a kDNA minicircle sequence of a mediterrean region isolate of L. infantum (Genebank A/N AF169140). Briefly, 2 µl of each DNA sample were added to a reaction mix consisting of et al., 1984) and Spain (Fisa et al., 1999), and black rats in Italy (Gradoni et al., 1983) and Spain (Morillas-Márquez et al., 1985), which have been considered wild reservoirs. Cats in Portugal (Durão et al., 1994), Spain (Hervas et al., 1999), France (Ozon et al., 1998) and Italy (Poli et al., 2002;Pennisi et al., 2004), and a wolf in Portugal (Rebelo, personal communication) have also been found parasitized and generally considered as accidental hosts. Horses infected with Leishmania have been detected in South and Central America (Aguilar et al., 1984;Oliveira-Neto et al., 1988;Ramos-Vara et al. 1996), Germany (Koehler et al., 2002) and Spain (Solano-Gállego et al., 2003). In this report, we describe the first autochthonous case of equine infection due to L. infantum in Portugal. a final volume of 20 µl. Optimal conditions for PCR amplification were: 95º C for 10 min and 40 cycles consisting of 95º C for 15 sec and 60º C for 60 sec. The PCR reactions were performed in the ABI PRISM ' 5700 System (Perkin-Elmer, Applied Biosystems). One of the horses presented a single irregular ulcerative skin lesion of 2.5 × 1 cm in size in the right metatarsus, which evolved from a small erosion within two months. Analysis by CIE (Fig. 1) revealed the presence of anti-Leishmania antibodies in the horse se...

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“…gênero Acomys e Rattus com lesões dermatológicas compatíveis com LT. Infelizmente o parasito ainda não foi isolado, sendo identificado apenas o gênero Leishmania (HOOGSTRAAL et al, 1963;PETROVIC et al, 1975;BETTINI et al, 1980;LAINSON et al, 1981;GRAMICCIA et al, 1982;AZAB et al, 1984;IBRAHIM et al, 1992;KERR et al, 1995).…”

Section: Referencial Teóricounclassified

Aspectos Da Toxoplasmose E Tripanossomíase Em Animais E Sua Importância Em Saúde Única

Santos¹,

Santos²,

Silva³

et al. 2023

Multiplicidade Das Ciências Da Saúde, Volume 4

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Esta obra está licenciada com uma Licença Creative Commons Atribuição 4.0 Internacional (CC BY 4.0).O conteúdo desta obra e seus dados em sua forma, correção e confiabilidade são de responsabilidade exclusiva dos autores. Permitido o download da obra e o compartilhamento desde que sejam atribuídos créditos aos autores, mas sem a possibilidade de alterá-la de nenhuma forma ou utilizá-la para fins comerciais.

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Typage enzymatique de onze souches de Leishmania isolées, en Italie continentale, à partir de formes viscérales murines, canines et vulpines

Abstract: A nnales de Parasitologie (Paris ; 1982, t. 57, n° 6, pp. 527

Cited by 30 publications

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The First Isoenzymatic Characterizations of the Leishmania Strains Responsible for Cutaneous Leishmaniasis in the Area of Annaba (Eastern Algeria)

The First Isoenzymatic Characterizations of the Leishmania Strains Responsible for Cutaneous Leishmaniasis in the Area of Annaba (Eastern Algeria)

Equine infection withLeishmaniain Portugal

Aspectos Da Toxoplasmose E Tripanossomíase Em Animais E Sua Importância Em Saúde Única

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