No abstract

Responses to drought stress by water withholding have been studied in 1 year old Holm oak (Quercus ilex subsp. ballota [Desf.] Samp.) seedlings from seven provenances from Andalusia (southern Spain). Several physiological parameters, including predawn xylem water potentials and relative water content in soil, roots, and leaves as well as maximum quantum efficiency and yield of PSII were evaluated for 28 days in both irrigated and nonirrigated seedlings. The leaf proteome map of the two provenances that show the extreme responses (Seville, GSE, is the most susceptible, while Almerı́a, SSA, is the least susceptible) was obtained. Statistically significant variable spots among provenances and treatments were subjected to MALDI-TOF/TOF-MS/MS analysis for protein identification. In response to drought stress, ~12.4% of the reproducible spots varied significantly depending on the treatment and the population. These variable proteins were mainly chloroplastic and belonged to the metabolism and defense/stress functional categories. The 2-DE protein profile of nonirrigated seedlings was similar in both provenances. Physiological and proteomics data were generally in good agreement. The general trend was a decrease in protein abundance upon water withholding in both provenances, mainly in those involved in ATP synthesis and photosynthesis. This decrease, moreover, was most marked in the most susceptible population compared with the less susceptible one.

Question: Which is the best model to predict the habitat distribution of Buxus balearica Lam. in southern Spain? Location: Málaga and Granada, Spain, across an area of 38 180 km2. Methods: Prediction models based on 17 environmental variables were tested. Six methods were compared: multivariate adaptive regression spline (MARS), maximum entropy approach to modelling species' distributions (Maxent), two generic algorithms based on environmental metrics dissimilarity (BIOCLIM and DOMAIN), Genetic Algorithm for Rule‐set Prediction (GARP), and supervised learning methods based on generalized linear classifiers (support vector machines, SVMs). To test the predictive power of the models we used the Kappa index. Results: Maxent most accurately predicted the habitat distribution of B. balearica, followed by MARS models. The other models tested yielded lower accuracy values. A comparison of the predictive power of the models revealed that climate variables made the highest contributions among the environmental variables studied. The variables that made the lowest contributions were the insolation models. To examine the sensitivity of the models to a reduction in the number of variables, a test showed that accuracy of over 0.90 was maintained by applying just three climatic variables (spring rainfall, mean temperature of the warmest month, and mean temperature of the coldest month). Maps derived from the algorithms of all models tested coincided well with the known distribution of the species. Conclusions: Model habitat prediction is a preliminary step towards highlighting areas of high habitat suitability of B. balearica. These data support the results of previous research, which show that MaxEnt is the best technique for modelling species distributions with small sample sizes.

No abstract

Quercus ilex is one of the European forest species most susceptible to root rot caused by the oomycete Phytophthora cinnamomi. This disease contributes to holm oak decline, a particularly serious problem in the 'dehesas' ecosystem of the southwestern Iberian Peninsula. This work describes the host-pathogen interaction of Q. ilex and P. cinnamomi, using new infection indices at the tissue level. Fine roots of 6-month-old saplings inoculated with P. cinnamomi were examined by light microscopy and a random pool of images was analysed in order to calculate different indices based on the measured area of pathogen structures. In the early stages of invasion, P. cinnamomi colonizes the apoplast and penetrates cortical cells with somatic structures. On reaching the parenchymatous tissues of the central cylinder, the pathogen develops different reproductive and survival structures inside the cells and then expands through the vascular system of the root. Some host responses were identified, such as cell wall thickening, accumulation of phenolic compounds in the middle lamella of sclerenchyma tissues, and mucilage secretion blocking vascular cells. New insights into the behaviour of P. cinnamomi inside fine roots are described. Host responses fail due to rapid expansion of the pathogen and a change in its behaviour from biotrophic to necrotrophic.

Highlights • Mediterranean oaks are endangered by infection with an invasive alien oomycete. • Forecasts based on SDM showed an expansion of the plant pathogen within Andalusia. • Our SDMs verified the known environmental suitability and provided new insights. • Phytosanitary management zones may be set from the current and future distribution.

Santelices, R., F. Drake, C. Mena, R. Ordenes, and R.M. Navarro-Cerrillo. 2012. Current and potential distribution areas for Nothofagus alessandrii, an endangered tree species from central Chile. Cien. Inv. Agr. 39(3): 521-531. Nothofagus alessandrii ("ruil") is an endangered species native to the Maule Coastal Forest of central Chile. Previous studies have demonstrated that the current distribution of this species and rates of deforestation and fragmentation present a serious threat to the species. To address this threat, spatial and temporal variation in remnant "ruil" forest cover was analyzed for the period from 1991 to 2008. Using aerial photography and geomatic tools, the state of "ruil" forests cover in 1991 was compared to the "ruil" forests cover in 2008 and with this information, the potential distribution area of this species was calculated. The results demonstrate that the current area of "ruil" forest is approximately 314 ha, with a net loss of 42 ha (12% of the forested area) over the 17-year study period. The annual deforestation rate for "ruil" during this period was 0.74%, a value that is significantly lower than that reported just two decades ago. Taken together with the fact that the majority of "ruil" forests belong to large private companies, with an additional 15% protected by the Chilean government, the conservation status of "ruil" forest has become less critical, at least in the short term. In addition, the habitat model from this study showed that there is a large area of 9,841 ha within which "ruil" forests could be greatly expanded through active forest restoration.

Phytophthora cinnamomi Rands. is an important root rot pathogen widely distributed in the north hemisphere, with a large host range. Among others diseases, it is known to be a principal factor in the decline of holm oak and cork oak, the most important tree species in the “dehesa” ecosystem of south-western Spain. Previously, the focus of studies on P. cinnamomi and holm oak have been on molecular tools for identification, functional responses of the host, together with other physiological and morphological host variables. However, a microscopic index to describe the degree of infection and colonization in the plant tissues has not yet been developed. A colonization or infection index would be a useful tool for studies that examine differences between individuals subjected to different treatments or to individuals belonging to different breeding accessions, together with their specific responses to the pathogen. This work presents a methodology based on the capture and digital treatment of microscopic images, using simple and accessible software, together with a range of variables that quantify the infection and colonization process.