Physiological and Proteomic Analyses of Drought Stress Response in Holm Oak Provenances

Abstract

Responses to drought stress by water withholding have been studied in 1 year old Holm oak (Quercus ilex subsp. ballota [Desf.] Samp.) seedlings from seven provenances from Andalusia (southern Spain). Several physiological parameters, including predawn xylem water potentials and relative water content in soil, roots, and leaves as well as maximum quantum efficiency and yield of PSII were evaluated for 28 days in both irrigated and nonirrigated seedlings. The leaf proteome map of the two provenances that show the extreme responses (Seville, GSE, is the most susceptible, while Almerı́a, SSA, is the least susceptible) was obtained. Statistically significant variable spots among provenances and treatments were subjected to MALDI-TOF/TOF-MS/MS analysis for protein identification. In response to drought stress, ~12.4% of the reproducible spots varied significantly depending on the treatment and the population. These variable proteins were mainly chloroplastic and belonged to the metabolism and defense/stress functional categories. The 2-DE protein profile of nonirrigated seedlings was similar in both provenances. Physiological and proteomics data were generally in good agreement. The general trend was a decrease in protein abundance upon water withholding in both provenances, mainly in those involved in ATP synthesis and photosynthesis. This decrease, moreover, was most marked in the most susceptible population compared with the less susceptible one.

Physiology and proteomics research on the leaves of ancient Platycladus orientalis (L.) during winter

“…These positional variations may be due to protein post-translational modifications (PTMs), different copies of a same gene or protein degradation [26]. Similar positional variations have also been reported in previous studies [27,28]. In addition, some of the specific spots were detected in more than one protein identification (Supplementary Table 3), which were possibly of comigration of multiple proteins [29].…”

“…There were five protein species belonging to the PR-5 group (spots 0014, 1010, 8001, 0012 and 0129) and two protein species belonging to PR-2 group (spots 3206 and 3208) identified. In our study, the allergen Jun a 3 (spots 0014, 8001 and 0012), thaumatinlike protein (spots 1010 and 0129) and glucanase-like protein (spots 3206 and 3208) were identified in multiple spots; post-translational modifications or different isoforms of the same gene product [27] may be present and performing overlapping roles during low temperature stress. However, most of the PR protein species identified were less abundance in T2 and T3 plants than T1 plants (except for spot 0129, which was only detected in T2 gels).…”

Comparative Proteomic Analysis of Brassica napus in Response to Drought Stress

“…In fact, proteins related to ATP synthesis, photosynthesis/carbon fixation, glycolysis, and stress and defense were commonly reported as drought and/or salt-responsive proteins from many other organisms 85 . In this study, the levels of proteins related to metabolism were increased in drought- 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 20 stressed samples, such as malate dehydrogenase, triosephosphate isomerase, glutamine synthetase, ATP synthase, and triosephosphate isomerase 12,30,52,84,86,87 Table 4 in the Supporting Information). In contrast to the increases in protein abundance, the activities of APX, CAT, SOD, and GST decreased in response to drought stress at the 10 th and 14 th day ( Figure 2).…”

“…The most commonly increased proteins under drought stress are involved in stress and defense, such as dehydrin, late embryogenesis abundant proteins (LEA), jacalin-related lectin 35, HSPs, 2-cys peroxiredoxin BAS1, and ROS scavenging enzymes, which include GST, APX, Cn/Zn SOD, and GPX12,30,52,84,86,88,89 . In this study, all of these proteins were increased in drought-stressed plants compared to control plants (Supplemental…”

Role of Proteomics in Crop Stress Tolerance

“…These proteins were associated with functions such as photosynthesis, signal transduction and regulation processes. Valero-Galván et al (2013) showed a decrease in protein abundance/expression mainly those involved in ATP synthesis and photosynthesis upon water withholding/water deficiency in holm oak ( Quercus ilex ).…”