Source and Description of Probe. A 1,486-bp rat cDNA clone for myogenin (MYOG) was excised from the EcoRI site of the plasmid, pBluescrybe M13-(Wright et al., 1989). Method of Detection. DNA was isolated from whole blood and digested with MspI. Fragments were then separated by agarose gel electrophoresis and transferred to charged nylon membranes. Hybridizations were at 65°C for 16 to 20 h (.5 M NaCl, .05 M Naphosphate buffer, pH 6.5, 5x Denhardt's reagent, 10% dextran sulfate, .5% SDS, 100 pg/mL sonicated, denatured salmon sperm DNA). Final washes were at 55 to 60°C in .7x SSC, 5% SDS for 15 to 20 min.Source and Description of Probe. A 1,486-bp rat cDNA clone for myogenin (MYOG) was excised from the EcoRI site of the plasmid, pBluescrybe M13-(Wright et al., 1989).
Source and Description of Probe. A 1,296-bp rat cDNA clone for MRF4 (also called MYF6) was excised from the EcoRI site of the plasmid pBluescript KS+ (Rhodes and Konieczny, 1989
SummaryPigs from a population consisting of eight US breeds or strains and three Chinese breeds were examined by restriction fragment length polymorphism (RFLP) analysis of the heat shock protein HSP70 gene(s). Limited polymorphisms with PstI and PvuII restriction enzymes were observed, but there were no polymorphisms with BomIII and BglI.
Source and Description of Probe. A 2.7-kb human cDNA clone for TAP1 was excised from the XbaI site of the plasmid pRSV.5neo (Spies et al., 1990). Method of Detection. Hybridizations were performed at 65°C for 16 to 20 h (10% dextran sulfate, 7% SDS, .263 M NazHP04, 1% BSA, 1 mM EDTA, 100 pg/mL sonicated denatured salmon sperm DNA). Final washes were done at 65°C in .7x SSC, .5% SDS for 15 to 20 min.
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