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Genetic improvement of sugarcane variety CoC 671 was carried out through somaclonal variation. The somaclones were obtained by developing callus on 0.8M EMS and 13.57M 2-4.D containing medium. All the 310 regenerated plants were hardened in green house and planted in field for preliminary selection. On the basis of biometric and biochemical parameters 17 somaclones were evaluated in rod row trial and then for smut resistance supplementing with smut inoculum and detection by PCR.The somaclonal variants TC 906 was resistant and TC 922 was moderately resistant to smut. Somaclone TC 922 (2.17 kg, 165 t/ha, 3.34 cm) and TC 906 (2.07 kg, 144.11 t/ha, 3.25 cm) were superior in single cane weight, cane yield and cane diameter over their donor CoC 671 (1.72 kg, 128 t/ha, 2.74 cm). The morphological variations viz. stem colour, internodal alignment, internodal waxiness and leaf width were distinct and stable. The present study reports beneficial traits of the somaclones derived from CoC 671 and their field performance, especially for smut resistance and yield traits and early maturity.

Combining ability, components of genetic variance and graphic analysis revealed that nodulation in the cultivars of Chickpea (Cicer arietinum L.) under study, was predominantly under the control of non-additive gene action although substantial additive effect was also present. The crosses giving high specific combining ability effects also manifested highly significant positive heterosis. The parents F-61, Giza and Annegiri possessed mostly dominant alleles while Phule G-5, NEC-249 and N-3 1 possessed mostly recessive alleles having positive effect on nodule weight,

Background: In the present study a statistical model (Response Surface Methodology) was proposed for optimization of siderophore production by using Enterobacter hormaechei.Methods: The rhizospheric soil was used for isolation and isolates were screened for siderophore production by chrome-azurol S (CAS) assay. One potent isolate producing maximum siderophore was selected and characterized by 16S rRNA gene sequencing. The culture conditions were optimized for maximum siderophore production by using Central Composite Design. The response surface curves were used to predict the optimum levels of the factors affecting the yield of siderophore. Result: By using rhizospheric soil,eight isolates were obtained and one potent organism was identified as Enterobacter hormaechei subsp. oharae (Accession No. MT 775835) by BLAST. The maximum siderophore production (98%) was obtained in succinate medium and the optimum values of variables were found as pH 7, time 60 hrs, temp. 28°C and succinic acid conc. 0.40%. RSM was used to analyze the data by developing 3D surface plots and the residuals plots. ANOVA was used to determine regression coefficients.

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