The effect of donor plants annual cycle and anther/spike position on the production of microspore-derived plants and albinism were studied. We used the winter cv. Igri and the spring cv. Cork, known to respond similarly in anther culture but to produce 78% and 2% of green plants, respectively. In both cvs. the number of microspore-derived plants was significantly higher when the anthers were collected from January to July than from August to December. However, during this period the proportion of albino plants was not altered. Conversely, the anther response decreased from 76.6 to 31.5% in Igri and from 58.8 to 32.0% in Cork when the donor spike originates from the main shoot or the fourth tiller. Significantly, anthers collected from spike of the second tiller enabled us to drastically increase the proportion of regenerated green plantlets, by 16% in Igri and 1800% in Cork.

No abstract

High-performance liquid chromatography (HPLC) was used to identify and quantify fifteen amino acids and phenolic active compounds in the tubers of twelve potato (Solanum tuberosum L.) varieties: very early -'VB

Testing for alkaloids in lupine varieties was conducted at the Vokė Branch of Lithuanian Institute of Agriculture during a competitive trial of feeding lupine (Lupinus L.) in 2006-2007. Samples were taken from feeding yellow lupine (Lupinus luteus L.), 'Trakiai' and 'Vilčiai' varieties, the narrow-leaved lupine (Lupinus angustifolius L.), 'Vilniai' variety and from cropper No. 1702. Alkaloid content was estimated in the periods of bud formation, full blossom, shining legumes and full maturity. The test material included exsiccates of leaves, stems, inflorescences, legumes, and seeds.According to the results, in the 'Trakiai' and 'Vilčiai' varieties of forage yellow lupins (Lupinus luteus L.) total alkaloids were lower than in the 'Vilniai' variety of narrow-leaved lupins (Lupinus angustifolius L.) and variety N1702.The highest content of alkaloids was identified in the periods of full blossom and legumes. A lower content was noted during bud formation, and the lowest in the full maturity period.High levels of alkaloids are present in legumes, while stems, inflorescences and leaves contain lesser amounts. The lowest content of alkaloids was identified in seeds.

Testing of lupine varieties for alkaloids was performed in the period 2006-2010 at the Voke Branch of the Lithuanian Institute of Agriculture in the course of a competitive trial of feeding lupine (Lupinus sp.). Samples of feeding yellow lupine (Lupinus luteus L.) varieties as well as narrow-leaved lupine (Lupinus angustifolius L.) were used. The alkaloid concentration was assessed in the periods of inflorescence emergence (BBCH 51-55), flowering (BBCH 64-67), development of fruit (BBCH 71-75) and ripening of seed (BBCH 85-88). Exsiccates of leaves, stems, flowers, pods and seeds were used as test material. The results revealed similar average levels of alkaloids in yellow forage lupine (Lupinus luteus L.). Analysis of the average alkaloid levels showed that in all phenological stages in the stems of yellow lupine (Lupinus luteus L.) alkaloid levels were lower than in the narrow-leaved (Lupinus angustifolius L.). At the stage of flowering (BBCH 64-67), the average alkaloid level in the leaves was lower than in the stems. In the course of our investigation, the highest average alkaloid levels were determined in the pods (0.114 ± 0.007) and flowers (0.114 ± 0.006) of narrow-leaved lupines, and the lowest level in the seeds (0.022 ± 0.003) of yellow lupines

No abstract

The research was carried out at Vokė Branch of the Institute of Agriculture of Lithuanian Research Centre for Agriculture and Forestry in 2018–2019. The objective of this study was to determine the influence of the relative proportion of stems, leaves and flowers on biomass formation and grain yield in organic and conventional farming systems. The study found that buckwheat produced one-third more biomass in the conventional farming system than in the organic farming system. Differences between buckwheat cultivars were more distinct due to biomass formation than grain yield. The research determined that the productivity (biomass and grain yields) of buckwheat was dependent on the proportion of morphological elements in plants and the process of biomass formation and grain yield in organic and conventional agricultural systems. Biomass yields depended on the relative number of stems in both farming systems. Grain yield depended on the ratio of flowers in the morphostructure; however, statistically significant correlations were found only in the organic farming system.

It was determined that amounts of chlorogenic acid, tyrosine, and tryptophan in potato tubers depend on meteorological conditions, farming type, individual properties of a variety, and interaction of these factors (P<0.05). It was noted in 2011 that organically grown potato tubers accumulated signifi cantly higher amounts of chlorogenic acid, tyrosine, and tryptophan derivatives than conventionally grown tubers, while in 2012 the opposite was observed (P<0.01). Such results are due to the different meteorological conditions during the survey years.