The effect of donor plants annual cycle and anther/spike position on the production of microspore-derived plants and albinism were studied. We used the winter cv. Igri and the spring cv. Cork, known to respond similarly in anther culture but to produce 78% and 2% of green plants, respectively. In both cvs. the number of microspore-derived plants was significantly higher when the anthers were collected from January to July than from August to December. However, during this period the proportion of albino plants was not altered. Conversely, the anther response decreased from 76.6 to 31.5% in Igri and from 58.8 to 32.0% in Cork when the donor spike originates from the main shoot or the fourth tiller. Significantly, anthers collected from spike of the second tiller enabled us to drastically increase the proportion of regenerated green plantlets, by 16% in Igri and 1800% in Cork.

No abstract

High-performance liquid chromatography (HPLC) was used to identify and quantify fifteen amino acids and phenolic active compounds in the tubers of twelve potato (Solanum tuberosum L.) varieties: very early -'VB

Testing for alkaloids in lupine varieties was conducted at the Vokė Branch of Lithuanian Institute of Agriculture during a competitive trial of feeding lupine (Lupinus L.) in 2006-2007. Samples were taken from feeding yellow lupine (Lupinus luteus L.), 'Trakiai' and 'Vilčiai' varieties, the narrow-leaved lupine (Lupinus angustifolius L.), 'Vilniai' variety and from cropper No. 1702. Alkaloid content was estimated in the periods of bud formation, full blossom, shining legumes and full maturity. The test material included exsiccates of leaves, stems, inflorescences, legumes, and seeds.According to the results, in the 'Trakiai' and 'Vilčiai' varieties of forage yellow lupins (Lupinus luteus L.) total alkaloids were lower than in the 'Vilniai' variety of narrow-leaved lupins (Lupinus angustifolius L.) and variety N1702.The highest content of alkaloids was identified in the periods of full blossom and legumes. A lower content was noted during bud formation, and the lowest in the full maturity period.High levels of alkaloids are present in legumes, while stems, inflorescences and leaves contain lesser amounts. The lowest content of alkaloids was identified in seeds.

Testing of lupine varieties for alkaloids was performed in the period 2006-2010 at the Voke Branch of the Lithuanian Institute of Agriculture in the course of a competitive trial of feeding lupine (Lupinus sp.). Samples of feeding yellow lupine (Lupinus luteus L.) varieties as well as narrow-leaved lupine (Lupinus angustifolius L.) were used. The alkaloid concentration was assessed in the periods of inflorescence emergence (BBCH 51-55), flowering (BBCH 64-67), development of fruit (BBCH 71-75) and ripening of seed (BBCH 85-88). Exsiccates of leaves, stems, flowers, pods and seeds were used as test material. The results revealed similar average levels of alkaloids in yellow forage lupine (Lupinus luteus L.). Analysis of the average alkaloid levels showed that in all phenological stages in the stems of yellow lupine (Lupinus luteus L.) alkaloid levels were lower than in the narrow-leaved (Lupinus angustifolius L.). At the stage of flowering (BBCH 64-67), the average alkaloid level in the leaves was lower than in the stems. In the course of our investigation, the highest average alkaloid levels were determined in the pods (0.114 ± 0.007) and flowers (0.114 ± 0.006) of narrow-leaved lupines, and the lowest level in the seeds (0.022 ± 0.003) of yellow lupines