Introduction. The lack of dietary fibre in the Russian people diet contributes to the development of various diseases. In this regard, it seems worthwhile to enrich foods with dietary fibre obtained from various types of raw materials. In our experiments, we used beet pulp. This study aimed to develop a technology for obtaining combined dietary fibre using the electrophysical method and evaluate its detoxification properties. Study objects and methods. Study objects were pectin substances and combined detoxicants from beet pulp obtained by extracting with succinic acid with and without an extremely low-frequency electromagnetic field (ELF EM) treatment. The profiles of combined detoxicants and pectin substances were identified by IR-Fourier spectrometry. Beet pectin, beet cellulose, and their combined detoxicants were tested for complexing (binding) capacity with respect to lead ions (Pb2+). For this, we applied the trilonometric method with some modifications. Results and discussion. The analysis of the absorption bands of carboxyl groups carbonyls revealed the presence of free carboxyl groups in the combined detoxicants. The combined detoxicant with a 1:0.5 ratio of cellulose and pectin substances showed a high complexing (binding) capacity (601 mg/Pb2+) with respect to lead ions (Pb2+). Conclusion. We developed a technology for producing combined detoxicants with a high complexing capacity with respect to lead ions analysed microstructures of gels obtained during the interaction between the combined detoxicant and lead acetate solution.
This study purpose was to optimize the production technology of encapsulated functional detoxicants based on combined dietary fibers (DF) obtained from beet pulp according to the developed technology. Production technology optimization for functional encapsulated food products was performed using mathematical programming methods based on Statistica v.10 and MathСAD v.15 software environments. An optimal technological production mode for encapsulated food products with high quality indicators, having an increased detoxification ability in relation to heavy metal ions, including combined DF, obtained from beet pulp.
Оптимизированы технологические режимы получения комбинированных детоксикантов растительного происхождения с высокой связывающей способностью по отношению к ионам свинца. Комбинированные детоксиканты из свекловичного жома получали по модифицированной авторами технологии. Многокритериальную оптимизацию технологических режимов получения комбинированных детоксикантов проводили методами математического программирования, реализованными средствами программных сред Statistica 10 и Mathсad 15. Определены оптимальные технологические режимы производства комбинированных детоксикантов из свекловичного жома при гидролизеэкстрагировании пульпы: рН среды 2,5 2,8 температура 60 80С продолжительность гидролизаэкстрагирования при обработке ЭМП КНЧ 40 65 мин массовые доли янтарной и лимонной кислот в растворе гидролизующего агента 70 100 и 0 30 соответственно. Экспериментально установлено оптимальное соотношение клетчатки и пектиновых веществ соответственно 1 : (0,4 0,5), обеспечивающее высокую комплексообразующую способность комбинированного детоксиканта. Установлено, что при данных технологических параметрах связывающая способность по отношению к ионам свинца повышается в среднем более чем на 20. Разработанная технология производства комбинированного детоксиканта с высокой комплексообразующей способностью по отношению к ионам свинца может быть использована для получения продуктов питания функционального назначения. Technological modes of the process of obtaining combined detoxicants of plant origin with a high binding capacity to lead ions are optimized. Combined detoxicants from beet pulp were obtained by the modified technology of the authors. Multicriteria optimization of technological modes of obtaining combined detoxicants was carried out by methods of mathematical programming, implemented by means of software environments Statistica 10 and Mathcad 15. Optimal technological modes of production of combined detoxicants from beet pulp during hydrolysisextraction of pulp are determined: pH 2,5 2,8 temperature 60 80C duration of hydrolysisextraction during extremely low frequency electromagnetic field treatment 40 65 min mass fractions of succinic and citric acids in the solution of hydrolyzing agent 70 100 and 0 30 respectively. Optimal ratio of fiber and pectin respectively 1 : (0,4 0,5), providing a high complexing ability of the combined detoxicant, experimentally established. It is proved that with these technological parameters, the increase in the binding capacity with respect to lead ions is on average more than 20. The developed technology for producing a combined detoxicant with high complexing ability in relation to lead ions can be used to produce functional food products.
Проведена медикобиологическая оценка детоксикационных свойств комбинированных пищевых волокон (КПВ) растительного происхождения, полученных по разработанной авторами технологии. В качестве объекта исследования были КПВ из свекловичного жома: после экстракции лимонной кислотой образец 1 и после обработки ЭМП КНЧ образец 2. Для определения детоксикационной активности КПВ сформировано 3 группы беспородных белых крыс по 20 голов в каждой, которые вместе с основным кормом получали свинец в виде свинцовой соли уксусной кислоты из расчета 25,0 мг свинца/кг массы тела. 1я и 2я опытные группы получали к корму добавку КПВ в количестве 0,6 мг/кг массы тела животного. Исследования проводили в течение 62 сут. По завершении эксперимента установлено, что у крыс 1й и 2й опытных групп, получавших КПВ из свекловичного жома, прирост живой массы тела превышал аналогичный показатель контрольной группы на 32,7 и 35,8 соответственно содержание свинца в крови снизилось относительно контрольной группы на 36,3 и 43,8 соответственно содержание свинца в печени соответственно в 1,8 и 2 раза ниже по сравнению с контрольной группой животных. Установлено, что детоксикационная активность КПВ после обработки ЭМП КНЧ выше, чем после экстракции лимонной кислотой. Biomedical assessment of detoxification properties of combined dietary fiber of plant origin, obtained by the technology developed by the authors, was carried out. Combined dietary fiber from beet pulp: after extraction with citric acid sample 1 and after treatment with an extremely low frequency electromagnetic field sample 2 were the object of study. To determine the detoxification activity of combined dietary fibers 3 groups of mongrel white rats with 20 heads in each were formed. All animals in the main feed added lead in the form of lead of salt acetic acid from calculation 25,0 mg lead/kg masses body rats. Combined dietary fibers were added to the animal feed of the 1st and 2nd experimental groups in the amount of 0,6 mg/kg of animal body weight. The studies were carried out for 62 days. At the end of the experiment, it was found that in rats of the 1st and 2nd experimental groups, where combined beet pulp fibers were added to the main food, the live weight gain exceeded the same indicator of the control group by 32,7 and 35,8, respectively the lead content in the blood decreased relative to the control group by 36,3 and 43,8, respectively the lead content in the liver, respectively, was 1,8 and 2 times lower compared to the control group of animals. It was found that the detoxification activity of combined dietary fibers after treatment with an electromagnetic field of extremely low frequency is higher than after extraction with citric acid.
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