The present studies were designed to investigate the mechanism of previously-reported nocturnal hyperinsulinemia in the pinealectomized rat. Isolated islets were obtained from anesthetized control, sham-pinealectomized and pinealectomized rats, with 5 rats per surgical groups, during the early dark phase of the daily lightdark cycle. Batches of 3 islets each were incubated in various combinations of 2, 10 or 30 mM glucose with control buffer, medium in which cerebral cortex or pineal glands had previously been incubated for 2 hours, or sonicates of these same tissues. Insulin released into the culture medium was measured by radioimmunoassay. A significant hypersecretion of insulin was demonstrable in the islets from the pinealectomized animals. A stimulatory effect of both pineal medium and sonicates upon insulin release was similarly observed. Neither of these effects displayed an interaction with the concentration of glucose in the islet incubation medium and they, therefore, appear to be mediated by a mechanism which operates independently of stimulation by glucose. These results indicate that the rat pineal gland can exert direct effects upon insulin release from the islets, possibly through a humoral route. Further studies are in progress to characterize the nature and mode of action of the insulinotropic agent present in and released from the pineal gland.
Alloxan-diabetic male rats were used to test effects of pinealectomy (PX) and of sham-pinealectomy (SPX) on blood glucose levels at mid-dark in the daily light-dark (LD 12:12) cycle. Animals received a diabetogenic dose of alloxan 10 days postoperatively. Blood glucose was measured on days -1,1,2,3,4,5,7,10 and 15 after 8-hour fasts. Pinealectomized (PX) and non-operated (C) animals were equivalent in their hyperglycemia following alloxan. SPX animals contrastingly showed significantly less response to alloxan than did animals of the other two groups. This difference of the SPX animals was in terms of lower hyperglycemia, better maintenance of body weight and of survival. It is concluded that intracranial surgery alone (SPX) and without visible brain damage can affect mechanisms of glucose homeostasis, and that at least in some circumstances such a surgical effect is not only different from effects of surgical pinealectomy (PX), but is also probably not pineal-dependent.
Pineocytomas have been induced in a high percentage of hamsters inoculated intracerebrally within 24 hours after birth with particular strains of a human papovavirus. Studies on biochemical and ultrastructural charactertistics and transformation of such experimentally induced pineal tumors have led to important conclusions and implications: (1) Many of the differentiated pineocytoma cells contained organelles and related structures that are characteristic of hamster pineocytes, and others that are reminiscent of possible phylogenetic precursors, including pineal photoreceptor cells. (2) An inverse relationship was noted between degree of cytological differentiation and level of hydroxyindole-O-methyltransferase (HIOMT) activity in the pineocytomas. (3) It is therefore apparent that even when pineal tumor formation leads to great increase in pinealocyte-like cells, their enzymatic capacity for synthesis fo melatonin, and possibly of other humoral products, may be only 4 to 7% of that of normal pinealocytes if they are of a relatively less differentiated type. A number of important and basic questions are accessible and remain to be investigated via experimental pineocytomas, such as: (1) nature and significance of cytological interactions within the pineal; (2) probable occurrences, correlations and transformations of other pineal biosynthetic and hormonal processes; (3) degree and nature of environmental (photic, circadian and circannual factors) and physiologic controls; and (4) kinds of modifications of pineal-related functions.
Serial sections of the brains of four adult male hyraxes revealed a hitherto undescribed ependymal organ near the middle of the midbrain aqueduct. This structural modification of the dorsal wall of the aqueduct is separate and different from both the subcommissural organ and the posterior collicular recess. It is characterized by: (1) an elaborate system of ridges and crypts,
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