Solid‐state nanochannels have revealed great abilities in the sensing of ions, small biomolecules, and biological macromolecules. However, the current platform requires pretreatment of real samples to extract targets, which may induce false signals due to complicated collection and addition processes. Although nanopore electrodes or nanopipettes have been successfully utilized in the detection of intracellular redox‐active species without sample preparation processes, the insertion of nanoprobes to living cells is inevitable. Here, a strategy is reported to monitor H2O2 released from living cells based on functionalized solid‐state nanochannels without an insertion procedure. In this strategy, aggregation‐induced emission luminogens (AIEgens) with enzyme‐responsive linkage properties are combined in solid‐state nanochannels. When H2O2 released from cervical cancer cells (HeLa), Tyr‐containing AIEgens (TT) will form horseradish peroxidase‐modulated (HRP‐modulated) linkages in the nanochannels. The formation of linkages can result in the effective blockade of nanochannels, hence via transmembrane ionic current. Owing to the aggregation of linkage products, a fluorescence signal can be observed. By using these dual‐signal‐output nanochannels, in situ and noninvasive detection of H2O2 is able to be achieved. Together with molecular dynamic (MD) simulations results, solid surface zeta potential and contact angle experiments, it is concluded that the blockage of nanochannels is the dominate factor for ionic currents as well as fluorescence change.
This study explored whether body specificity unconsciously influenced preferences for certain people. Participants were presented pictures of the heads of 2 persons who were described as having the similar personality, profession and family background. They were instructed to choose 1 in each pair as the preferred date, preferred friend, more charismatic boss or as the better national leader. The results showed body specificity had an influence on the selection preference on first impression. Participants tended to choose the character on their dominant-hand side. This study not only provided the first social psychological evidence for the body-specificity hypothesis, but also first demonstrated a role for body specificity in impression formation and selection preference.
Cationic lipid-like molecules (lipidoids) are widely used for in vitro and in vivo gene delivery. Nearly all lipidoids developed to date employ double-tail or multiple-tail structures for transfection. Single-tail lipidoids are seldom considered for transfection as they have low efficiency in gene delivery. So far, there is no detailed study on the contribution to transfection efficiency of single-tail lipidoids when combined with standard double-tail lipidoids. Here, we use combinatorial chemistry to synthesize 17 double-tail and 17 single-tail lipidoids using thiol-yne and thiol-ene click chemistry, respectively. HEK 293T cells were used to analyze transfection efficiency by fluorescence microscopy and calculated based on the percentage of cells transfected. The size and zeta potential of liposomes and lipoplexes were characterized by dynamic light scattering (DLS). Intracellular DNA delivery and trafficking was further examined using confocal microscopy. Our study shows that combining single with double-tail lipidoids increases uptake of lipoplexes, as well as cellular transfection efficiency.
Based on the theory of strategic alliances and social networks, this article empirically studies the relationship between partnership, information sharing, and sustainable performance through a questionnaire survey of Chinese sports equipment manufacturers. The findings show that partnerships have a positive impact on sustainable performance; that information sharing plays a role in mediating the relationships between trust, cooperation, and sustainable performance; and that government support can positively impact the effect of partnerships on sustainable performance. Through empirical research, this article proves the mechanism of the impact of partnership on alliance performance, further expands the theoretical basis for enterprises’ establishment of strategic alliances, and has important enlightening significance for enterprises within alliances aiming to rationally use the networks inside and outside their alliances to obtain knowledge and resources and improve their sustainable performance.
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