The R2R3MYB proteins comprise one of the largest families of transcription factors and play regulatory roles in developmental processes and defense responses in plants. However, there has been relatively little effort to systematically carry out comprehensive genomic and functional analyses of these genes in tomato (Solanum lycopersicum L.), a reference species for Solanaceae plants, and the model plant for fruit development. In this study, a total of 121 R2R3MYB genes were identified in the tomato genome released recently and further classified into 29 subgroups based on the phylogenetic analysis of the complete protein sequences. Phylogenetic comparison of the members of this superfamily among tomato, Arabidopsis, grape, rice, poplar, soybean, cucumber and apple revealed that the putative functions of some tomato R2R3MYB proteins were clustered into the Arabidopsis functional clades. The chromosome distribution pattern revealed that tomato R2R3MYB genes were enriched on several chromosomes and 52 % of the family members were tandemly duplicated genes. Tissue specificity or different expression levels of SlR2R3MYBs in different tissues suggested differential regulation of tissue development as well as metabolic regulation. The transcript abundance level analysis during abiotic conditions identified a group of R2R3MYB genes that responded to one or more treatments suggesting that the SlR2R3MYBs played major roles in the plant response to abiotic conditions and involved in signal transduction pathways. This study not only provides a solid foundation for further functional dissection of tomato R2R3MYB family genes, but may also be profitable for, in the future, the improvement of tomato stress tolerance and fruit quality.

The indel in the promoter of CsHDZIV11 co-segregates with fruit spine density and could be used for molecular breeding in cucumber. Fruit spine density is an important quality trait for marketing in cucumber (Cucumis sativus L.). However, the molecular basis of fruit spine density in cucumber remains unclear. In this study, we isolated a mutant, few spines 1 (fs1), from CNS2 (wild type, WT), a North China-type cucumber with a high density of fruit spines. Genetic analysis showed that fs1 was controlled by a single recessive Mendelian factor. Bulked segregant analysis combined with genome resequencing were used for mapping fs1 in the F2 population derived from a cross between the fs1 mutant and WT, and it was located on chromosome 6 through association analysis. To develop more polymorphic markers to locate fs1, another F2 population was constructed from the cross between fs1 and 'Chinese long' 9930. Then, fs1 was narrowed down to a 110.4-kb genomic region containing 25 annotated genes. A fragment substitution was identified in the promoter region of Csa6M514870 between fs1 and WT. This fragment in fs1 was also present in wild cucumber. Csa6M514870 encodes a PDF2-related protein, a homeodomain-leucine zipper IV transcription factor (CsHDZIV11/CsGL3) sharing high identity and similarity with proteins related to trichome formation or epidermal cell differentiation. Quantitative reverse-transcription PCR revealed a higher expression level of CsHDZIV11 in young fruits from fs1 compared to WT. A molecular marker based on this indel co-segregated with the spine density. This work provides a solid foundation not only for understanding the molecular mechanism of fruit spine density, but also for molecular breeding in cucumber.

Coal seam gas, held within the inner pores of unmineable coal, is an important energy resource. Gas release largely depends on the gas seepage characteristics and their evolution within granular coal. To monitor this evolution, a series of experiments were conducted to study the effects of applied compressive stress and original grain size distribution (GSD) on the variations in the gas seepage characteristics of granular coal samples. Grain crushing under higher stress rates was observed to be more intense. Isolated fractures in the larger diameter fractions transformed from self-extending to inter-connecting pathways at a critical compressive stress. Grain crushing was mainly caused by compression and high-speed impact. Based on the test results of the original GSD effect, the overall process of porosity and permeability evolution during compression can be divided into three different phases: (1) rapid reduction in the void ratio; (2) continued reduction in the void ratio and large particle crushing; and (3) continued crushing of large particles. Void size reduction and particle crushing were mainly attributed to the porosity and permeability decreases that occurred. The performance of an empirical model, for porosity and permeability evolution, was also investigated. The predictive results indicate that grain crushing caused permeability increases during compression, and that this appeared to be the main cause for the predictive values being lower than those obtained from the experimental tests. The predictive accuracy would be the same for samples under different stress rates and the lowest for the sample with the highest proportion of large grain diameters.Significant experimental, numerical, or theoretical methods were conducted to understand the properties of granular coal, such as density, ash, and particle size effects on the fixed characteristics [7-11], coal particle moving [12], fragmentation behavior [13], coal combustion [14], etc. Adánez et al. [7] conducted a series of experiments and proposed an equation to evaluate the transport velocities of sand and coal particles. For coal blocks having larger particle diameters, the gas desorbs from its micropores, diffuses into macropores, and then seeps under a pressure gradient [15,16]. Hu et al. [17] experimentally and numerically investigated the scale effects and formation mechanism of gas releases from coal particles, and a bidisperse diffusion model was established to predict the experimental results; they found that the scale effects of gas releases from coal are controlled by the multi-scale pore structure of coal. In studies conducted using coal core samples as opposed to granular coal, it was found that under constant total stress conditions, the permeability for adsorbed gases increases when the pore pressure is reduced due to coal swelling [18][19][20], and decreases with increasing pore pressure due to matrix shrinkage [21].Gas permeability is also influenced by fracture geometry [6,22], fracture geometry and water-content [23], and the presen...

Using an enlarged alphabet of K-tuples is the way to carry out alignment-free comparison of genomes in the composition vector (CV) approach to prokaryotic phylogeny. We summarize the known aspects concerning the choice of K and examine the results of using CVs with subtraction of a statistical background for K=3-9 and using raw CVs without subtraction for K=1-12. The criterion for evaluation consists in direct comparison with taxonomy. For prokaryotes the best performances are obtained for K=5 and 6 with subtraction and for K=11, 12 or even more without subtraction. In general, CVs with subtractions are slightly better and less CPU consuming, but CVs without subtraction may provide complementary information.

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Antagonism between jasmonic acid (JA) and salicylic acid (SA) plays pivotal roles in the fine-tuning of plant immunity against pathogen infection. In this study, we compared the phytohormonal responses to Xanthomonas citri subsp. citri (Xcc) between the citrus canker-susceptible (S) cultivar Wanjincheng orange (Citrus sinensis Osbeck) and-resistant (R) cultivar Jindan (Fortunella crassifolia Swingle). Upon Xcc infection, SA and JA were strongly induced in Jindan (R) and Wanjincheng orange (S), respectively, and JA appeared to contribute to citrus disease susceptibility by antagonizing SAmediated effective defenses. A homologous gene encoding the allene oxide synthase (AOS) 1-2 enzyme, which catalyzes the first committed step in JA biosynthesis, was specifically upregulated in Wanjincheng orange (S) but not in Jindan (R). A promoter sequence analysis showed that abscisic acid (ABA)-responsive elements are enriched in the AOS1-2 of Wanjincheng orange (S) but not in Jindan (R). Accordingly, ABA treatments could induce AOS1-2 expression and JA accumulation, leading to enhanced citrus disease susceptibility in Wanjincheng orange (S), while the synthesis inhibitor sodium tungstate had the opposite effects. Moreover, ABA was specifically induced by Xcc infection in Wanjincheng orange (S) but not in Jindan (R). Thus, Xcc appeared to hijack host ABA biosynthesis to promote JA accumulation, which in turn suppressed effectual SA-mediated defenses to favor disease development in citrus. Our findings provide new insights into the molecular mechanisms underlying the differential citrus-canker resistance in citrus cultivars, and a new strategy for the biotechnological improvement of citrus canker resistance was discussed.

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In order to study the influence of roof stress and fracture distribution characteristics on the stability of stope surrounding rock under repeated mining in close-distance coal seams, the research background of this paper is the close-distance coal seam mining of a mine in Guizhou province, China. The dynamic evolution characteristics of stope roof caving and the stress environment change law under repeated mining are studied by using the method of similar simulation experiment, numerical simulation, and field verification. The results show that the roof stage under repeated mining can be divided into four stages: normal mining, roof deterioration, end face roof leaks, and support crushing. The tip-to-face distance, support height, and support working condition are the main influencing factors in end face roof control. The main reason for the support crushing is that the roof above the support is broken, the main roof cannot form the self-stable structure, and the support force is insufficient. The roof dynamic load is high, and the subsidence of the end face roof is large, which is prone to roof caving during the stope roof weighting. Moreover, the roof weighting is frequent under repeated mining, which leads to the broken roof and the support cannot be supported, resulting in the occurrence of the support crushing accident. The control strategies for stope roof under repeated mining are provided based on the thorough study findings. The working face 17101 roof is successfully controlled by the aforesaid procedures, which provides the foundation for the management of stope surrounding rock under repeated loading.