Objective: It is known that local or systemic injection of hemostatic drugs is used to reduce blood loss and number of blood transfusions in patients with hypocoagulation and hemorrhagic diathesis. However, the analysis of literature data shows that the drugs applied for the control of bleeding, traditionally used in medical practice, are not effective enough. This study deals with the systemic hemostatic activity of bis (2-aminoethan-1- sulfonate) calcium in the experiment.Methods: Experimental work in vitro is performed on the blood of healthy male donors, under conditions in vivo it is done on intraperitoneal injection in male rats. Thromboelastography was carried out with apparatus Thromboelastography (TEG) 5000 (Haemoscope Corporation, United States). The influence of first synthesized derivative and ethamsylate on functional activity of platelets was studied using a platelet aggregation analyzer “Biola 230LA” (Russia). Experimental evaluation of the system specific hemostatic activity in vivo was carried out using the model of parenchymatous bleeding in mature male rats. The interference came amid registration of bleeding stop time and extent of blood loss.Results: Bis (2-aminoethan-1-sulfonate) calcium shows procoagulation and proaggregant activity both in vitro and in vivo. Proaggregatory effect of bis (2-aminoethan-1-sulfonate) calcium is successfully implemented in the systemic hemostatic activity in terms of parenchymal bleeding, surpassing the control group and the group of etamsylate.Conclusion: The results of these studies reveal potentially high systemic hemostatic activity of bis (2-aminoethan-1-sulfonate) calcium, urging the need for further study on this compound and its analogs to create on their basis highly efficient, selective correctors of the hemostatic system.
Although the total content of organic s compounds in water is regulated, it is rather difficult to formulate and set standards for individual organic substances coming to the biological treatment facilities of urban aeration stations. Many experts believe it necessary to conduct further experimental studies to set their permissible concentrations and prevent slowing down biological treatment. One of the accepted experimental control methods for toxicity is a bio testing method. In the course of the present study, an investigation into ampicillin toxicity was performed for the test-objects daphnia and community of microorganisms in active silt. The experiment was carried out on crustaceans using N.S. Stroganov standard technique. The impact of antibiotics on active silt was assessed by changes in the microorganisms’ dehydrogenases activity. The research results allow to characterize ampicillin as very low toxic to Daphnia and to active silt community of microorganisms , toxic effect of the antibiotics was established in concentrations of 200 to 400 mg/l.
Changes in the peptide composition of hemolymph of Galleria mellonella larvae induced by their immunization have been studied, and some new peptides have been found. The composition of fractions exhibiting antibacterial activity was investigated. Known antibacterial peptides have been found in the hemolymph of control larvae and those immunized with bacteria.
Due to the continuous reduction of the areas for crops and increase of urbanpopulation, the increase in productivity of crops is especially importantaspect in production of food today. One of problems of agriculture is creationof conditions for receiving a crop. Quality of a crop of various crops depends onefficiency of preseeding processing of seeds. This research investigated a methodof impact of preseeding processing of seeds of flax by a complex of physicalfactors: ultra-violet radiation, ozone and the magnetic field (MF) with induktionof 100, 200, 250 and 300 Gs on growth and activity of a catalase andperoxidase of a plant, and also the maintenance of photosynthetic pigments inflax sprouts.
The fractions containing antimicrobic peptides have been purified from a haemolymph of caterpillars Galleria mellonella by chromatographic methods and studied by mass spectrometry.
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