Study of peptide fractions from hemolymph of Galleria mellonella

Sribnaya, O. S.; Purygin, Pyotr P.; Klenova, N. A.; Buryak, А. К.; Литвинова, Е. Г.

doi:10.1134/s0006297910090117

Cited by 2 publications

(1 citation statement)

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“…Insect defence peptides possess distinct activities directed against bacteria and fungi. Many antimicrobial peptides and other immunerelated proteins have also been described in the greater wax moth Galleria mellonella [4][5][6][7][8][9][10][11]. This insect model is widely used for studies concerning different aspects of host-pathogen interactions [12][13][14][15][16][17][18].…”

Section: Introductionmentioning

confidence: 99%

Galleria mellonella infected with Bacillus thuringiensis involves Hsp90

Wojda

Kowalski

2013

Open Life Sciences

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Insects are good models for studying the innate immune response. We report that Galleria mellonella larvae infected with entomopathogenic bacteria Bacillus thuringiensis kurstaki show changes in the level of Hsp90. Our experimental approach was to pre-treat larvae with the Hsp90-binding compound, 17-DMAG, before infection with B. thuringiensis. We show that pre-treated animals display a higher level of immune response. This was mainly manifested by enhanced action of their hemolymph directed toward living bacteria as well as lysozyme activity digesting bacterial peptidoglycan. The observed phenomenon was due to the higher activity of antimicrobial peptides which, in contrast to healthy animals, was detected in the hemolymph of the immunestimulated larvae. Finally, the physiological significance of our observation was highlighted by the fact that G. mellonella pre-treated with 17-DMAG showed a prolonged survival rate after infection with B. thuringiensis than the control animals. Our report points to a role for Hsp90 in the immune response of G. mellonella after infection with B. thuringiensis at the optimal growth temperature.

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