Changes in the peptide composition of hemolymph of Galleria mellonella larvae induced by their immunization have been studied, and some new peptides have been found. The composition of fractions exhibiting antibacterial activity was investigated. Known antibacterial peptides have been found in the hemolymph of control larvae and those immunized with bacteria.
The fractions containing antimicrobic peptides have been purified from a haemolymph of caterpillars Galleria mellonella by chromatographic methods and studied by mass spectrometry.
The antibacterial activity of hemolymph fractions isolated from Galleria mellonella larvae has been studied. Protein fractions possessing antibacterial activity were obtained using ion-exchange chromatography and gel electrophoresis techniques. MALDI-TOF mass-spectrometric analysis of these fractions showed the presence of a peptide with molecular mass 5627 Da that probably accounts for the antibacterial activity of the hemolymph. The obtained data confirm the expediency of using G. mellonella larvae hemolymph preparations (including homeopathic drugs) in medicine.
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