Penelitian ini bertujuan untuk mengetahui berat molekul dan sebaran protein reseptor Growth Hormon (GHR). Sampel yang dipakai adalah otot rib eye sapi bali dan sapi Wagyu. Pemeriksaan berat molekul protein reseptor memakai metode SDS-PAGE, Western Blotting, dan pengecatan Imunohisto kimia. Hasil penelitian menunjukkan, bahwa berat protein reseptor Growth Hormon adalah 76 Kdalton pada kedua jenis sapi tersebut dan rerata nilai IHK-Score untuk otot sapi wagyu adalah 234,5 dan pada sapi Bali adalah 199,6. Dapat disimpulkan, bahwa berat molekul protein reseptor Growth Hormon otot rib eye pada kedua sapi Wagyu dan sapi bali adalah sama dengan sebaran yang tidak sama.

Bovine Viral Diarrhea (BVD) or malignant diarrhea in cattle is one of the animal diseases that causes economic losses in the cattle industry worldwide. The biggest economic loss due to infection by Bovine Viral Diarrhea is related to reproductive and calf disorders that continue to transmit the virus to other cattle. This study aims to determine the presence of Bovine Viral Diarrhea antibodies in Bali cattle. The sample uses Bali cattle’s blood from 30 cows that are accommodated in 2 tubes that contain anti-coagulant and which do not contain anti-coagulant. After being processed to get serum, plasma and buffy coat cells, then the samples were examined using the ELISA method and presented descriptively. The results showed that there were positive suspects in Sobangan village, Badung with 8 out of 15 samples (53%) and positive suspects in the village of Payangan, Gianyar with 3 out of 15 samples (20%). Positive results are influenced by biosecurity of each type of sample farm, in Sobangan village is a large farm so biosecurity is difficult to implement and the spread of disease is faster than in Payangan village that have small farm type.

Since its discovery at the end of 2019, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has rapidly evolved into many variants, including the subvariant BA.2 and the GKA clade. Genomic clarification is needed for better management of the current pandemic as well as the possible reemergence of novel variants. The sequence of the reference genome Wuhan-Hu-1 and approximately 20 representatives of each variant were downloaded from GenBank and GISAID. Two representatives with no track of in-definitive nucleotides were selected. The sequences were aligned using muscle. The location of insertion/deletion (indel) in the genome was mapped following the open reading frame (ORF) of Wuhan-Hu-1. The phylogeny of the spike protein coding region was constructed using the maximum likelihood method. Amino acid substitutions in all ORFs were analyzed separately. There are two indel sites in ORF1AB, eight in spike, and one each in ORF3A, matrix (MA), nucleoprotein (NP), and the 3′-untranslated regions (3′UTR). Some indel sites and residues/substitutions are not unique, and some are variant-specific. The phylogeny shows that Omicron, Deltacron, and BA2 are clustered together and separated from other variants with 100% bootstrap support. In conclusion, whole-genome comparison of representatives of all variants revealed indel patterns that are specific to SARS-CoV-2 variants or subvariants. Polymorphic amino acid comparison across all coding regions also showed amino acid residues shared by specific groups of variants. Finally, the higher transmissibility of BA.2 might be due at least in part to the 48 nucleotide deletions in the 3′UTR, while the seem-to-be extinction of GKA clade is due to the lack of genetic advantages as a consequence of amino acid substitutions in various genes.

Malasseziosis is a common fungal infection in dogs and it is secondary to the initial underlying dermatitis infection. These infections can worsen the prognosis of disease in dogs. The study was conducted on 26 dogs that were treated in several veterinary clinics in the Badung, Bali. This study aimed to determine the incidence rate of Malassezia sp. in dogs with dermatitis. Samples were collected using tape smear method from the skin lesion and then by microscopic examination. The results were tabulated and analyze descriptively. The results showed that 15 of the 26 samples of dogs tested positive were infected with Malassezia sp. (58%). Infection is more common in male (60%) and geriatric dogs (40%). Lesions were more common in the ear, limb, vaginal and inguinal region of infected dogs

The high number of broken eggshells on laying hens in Indonesia is very detrimental to farmers, so that needs to be overcome. A total of 480 Lohman chickens with 40 weeks of age were divided into 4 treatment groups with the addition of different doses of Avizyme® 1502 respectively P0 as a control that got 0 g/Kg of feed, P1 got 1 g/Kg of feed, P2 got 2 g/Kg of feed, P3 got 3 g/Kg of feed. Avizyme® 1502 contains enzymes xylanase, amylase, and protease. This treatment was given daily for 30 days. The sampling of eggshell checking was recorded when start of administering enzymes to the end of the administration and at the end of the study, 6 eggs from each treatment were taken randomly to measure the protein level. The results showed that the addition of 1 g/Kg of Avizyme® 1502 on feed provided the highest protein level in eggs. During the research period, eggs produced by the groups of chicken under treatments of Avizyme 1502® showed a decrease in cracked eggshell.