Ekspresi Gen Reseptor Growth Hormon pada Otot Sapi Bali dan Sapi Wagyu

Suwiti, Ni Ketut; Sudipa, Putu Henrywaesa; Setiasih, Ni Luh Eka

doi:10.24843/bulvet.2022.v14.i02.p02

Cited by 9 publications

(20 citation statements)

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“…Tunika serosa merupakan lapisan paling luar yang terdiri dari jaringan ikat longgar, jaringan lemak, dan pembuluh darah. Suwiti et al (2010), pada sapi bali, dan Kadam et al (2007), pada kambing menemukan komponen yang sama pada tunika muskularis dan serosa sapi aceh, namun berbeda dengan yang dilaporkan Singh et al (2012), pada kerbau yang menyatakan bahwa pada tunika muskularis sekum kerbau hanya terdapat otot polos transversal.…”

Section: Pembahasanunclassified

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Struktur Histologi dan Histomorfometri Sekum Sapi Bali pada Bagian Basis, Corpus Dan Apex

Kencana

Setiasih

Heryani³

2023

bulvet

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Penelitian ini bertujuan untuk mengetahui struktur histologi dan histomorfometri serta perbedaan histomorfometri sekum sapi bali pada bagian basis, corpus dan apex. Pada penelitian ini 10 ekor sapi bali betina berusia empat sampai lima tahun diambil di Rumah Potong Hewan Pesanggaran. Cara pengambilan sampel adalah sampel sekum diambil pada bagian basis, corpus dan apex untuk selanjutnya difiksasi menggunakan larutan Neutral Buffered Formalin 10%, kemudian diberi pewarnaan menggunakan Haematoxillin-Eosin untuk dijadikan preparat histologi. Hasil pengamatan struktur histologi disajikan secara deskriptif kualitatif, sedangkan data histomorfometri disajikan secara deskriptif kuantitatif. Hasil penelitian menemukan sekum tersusun atas 4 lapisan; tunika mukosa, submukosa, muskularis dan serosa. Pengukuran histomorfometri menunjukkan ketebalan tunika mukosa, submukosa, muskularis dan serosa pada bagian basis berturut-turut 376,87±34,411?m, 1508,73±349,0222?m, 2767,76±609,698?m, 199,95±21,502?m, pada bagian corpus berturut-turut 380,36±51,501?m, 739,28±129,371?m, 2287,66±303,987?m, 328,19±77,468?m dan pada bagian apex berturut-turut 407,05±63,902?m, 615,57±205,736?m, 2730,51±332,044?m, 297,82±51,211?m. Histomorfometri tunika mukosa, tunika submukosa, tunika muskularis dan tunika serosa menunjukan bahwa tidak ada perbedaan yang signifikan pada tunika mukosa bagian basis, corpus dan apex, ketebal tunika submukosa bagian basis lebih tebal dibandingkan dengan bagian corpus dan apex, ketebal tunika muskularis bagian basis dan apex lebih tebal dibandingkan dengan bagian corpus, pada tunika serosa bagian basis, corpus dan apex tidak terdapat perbedaan yang signifikan. Perlu dilakukan penelitian anatomi makro dan mikro terhadap usus besar sapi bali dengan memperhatikan manajemen pemeliharaan, membedakan umur, jenis kelamin, dan melakukan pewarnaan khusus.

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Section: Pembahasanunclassified

“…Tidak adanya perbedaan ketebalan ini disebabkan oleh permukaan epitel yang terlihat merata pada tunika mukosa sekum bagian basis, corpus dan apex. Suwiti et al (2010) melaporkan bahwa pada sekum biasanya tidak dijumpai sel paneth, tidak memiliki plika maupun villi, sehingga epitelnya tampak lebih rata jika dibandingkan dengan usus halus.…”

Section: Pembahasanunclassified

Struktur Histologi dan Histomorfometri Sekum Sapi Bali pada Bagian Basis, Corpus Dan Apex

Kencana

Setiasih

Heryani³

2023

bulvet

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“…These parameters are not comprehensive, as they do not take into account the weight of muscle, bone and body fat, thereby affecting the assessment of quantity and quality. Previous studies by Suwiti et al (2015) and Safitri et al (2018) have only focused on the muscle microstructure and physical quality of Bali cattle meat, without any correlation to the physical appearance of the livestock as breeding stock for selection. Suwiti et al (2015) and Safitri et al (2018) did not also specifically assess the amount and type of constituent connective tissue.…”

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“…Previous studies by Suwiti et al (2015) and Safitri et al (2018) have only focused on the muscle microstructure and physical quality of Bali cattle meat, without any correlation to the physical appearance of the livestock as breeding stock for selection. Suwiti et al (2015) and Safitri et al (2018) did not also specifically assess the amount and type of constituent connective tissue. Therefore, this study aims to evaluate the correlation between the muscle score of Bali cattle and the quality of semitendinosus muscle microstructure in terms of myofibril morphometric and connective tissue composition using a histochemistry approach.…”

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Preliminary examination of the histochemistry of the semitendinosus muscle microstructure in Bali cattle (Bos javanicus) and the correlations with muscle score

Prawira,

Supratikno,

Anwar

et al. 2024

Anat Histol Embryol

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Bali cattle (Bos javanicus) are a local breed from Indonesia with the potential for beef production, but its meat has been reported to be tough. This indicates that there is a need to develop various methods that can be used for assessment to improve the quality of the meat. One such method is muscle scoring (MS), which is often used to predict the amount of meat in the carcass from live animals, but the muscle microstructure contributing to the quality is often neglected. Therefore, this study aims to evaluate the correlation between the muscle score of Bali cattle and the quality of muscle microstructure in terms of myofibril morphometric and connective tissue composition using a histochemistry approach. The population consisted of 31 Bali cattle, and MS was performed by evaluating the muscle line using a range of 1 to 15. The three individuals with the highest scores (Hi) and the three with the lowest (Lo) were sampled for semitendinosus muscle microstructural observation. The results showed that cattle in the Hi group had a higher surface area of myofibril and sarcomere length, but had a significantly lower relative percentage of intramuscular collagen compared to others in the Lo group (p < 0.05). Generally, muscle score had a positive correlation with body weight, surface area of myofibril and sarcomere length. In all cattle, the fibres identified were similar, where the thick types were dominant in the epimysium and perimysium, followed by the thin variants. Furthermore, the endomysium prominently contained non‐fibrous or other types of fibre. This study can provide the basic data that can be used as a pilot for future studies of muscle score assessment in Indonesian cattle.

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Diversity of SNP c.795A>G PLAG1 Gene and its Association to Birth Weight of Bali Cattle

Fahira,

Noor,

Jakaria

2022

JITV

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PLAG1 gene is one of those that regulate growth and body size. Â This study aimedto look at the PLAG1 gene polymorphism and its relationship to birth weight in Bali cattle using PCR-RFLP. The total sample used was 104 samples consisting of 66 Bali cattle from BPTU-HPT Denpasar and 38 Bali cattle from BPT-HMT Serading, each of which had birth weight data. PLAG1 gene polymorphism was analyzed using PCR-RFLP and the Sac1 restriction enzyme. The genotype and allele frequencies, heterozygosity, and Hardy-Weinberg equilibrium were all examined using Popgen32. General Linear Model was used to analyze the association of SNP 795AG PLAG1 gene with birth weight in Bali cattle. Amplification of the PLAG1 gene resulted in 776 bp fragments and two alleles. The PLAG1 gene had three genotypes: AA (562 bp and 182 bp), AG (562 bp, 182 bp, and 104 bp), and GG (562 bp, 182 bp, and 104 bp). Based on the results, the PLAG1 gene in Bali cattle was polymorphic. The alleles frequency of Bali cattle wasin Hardy-Weinberg equilibrium. The SNP c.795AG PLAG1 gene genotype were associated with birth weight in Bali cattle. The A allele is a determinant of high birth weight in Bali cattle where the AG genotype has the highest birth weight.

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Ekspresi Gen Reseptor Growth Hormon pada Otot Sapi Bali dan Sapi Wagyu

Cited by 9 publications

References 0 publications

Struktur Histologi dan Histomorfometri Sekum Sapi Bali pada Bagian Basis, Corpus Dan Apex

Struktur Histologi dan Histomorfometri Sekum Sapi Bali pada Bagian Basis, Corpus Dan Apex

Preliminary examination of the histochemistry of the semitendinosus muscle microstructure in Bali cattle (Bos javanicus) and the correlations with muscle score

Diversity of SNP c.795A>G PLAG1 Gene and its Association to Birth Weight of Bali Cattle

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