Objective: To explore the effect of glycerol at different concentrations using different extenders on DNA fragmentation and motility of frozen-thawed Kintamani Bali dog spermatozoa. Materials and Methods: Sample was collected from four mature Kintamani Bali dogs. Each ejaculate was prepared for cryopreservation with two different semen extenders; egg yolk Tris extender and coconut water-based extender. For each extender, three different glycerol concentrations were used; 4%, 6%, and 8%. Each of the six aliquots was loaded into 0.5 ml cryotube, placed on a styrofoam box 5 cm over liquid nitrogen for 10 min, and immersed in liquid nitrogen up to 8 min. Then, the frozen cryotubes were transferred into liquid nitrogen container. The cryotubes were thawed in a water bath at 38.5°C for 120 sec. After equilibration and thawing, each sample was assessed for motility parameters and for DNA fragmentation. Results: The addition of 6% glycerol to extenders revealed the most effective addition of glycerol on motility and sperm DNA fragmentation after equilibrium and post-thawing. Conclusion: It is concluded that both extenders with the addition of 6% glycerol are safe to be used as an extender in Kintamani Bali dog semen preservation, and DNA fragmentation of Kintamani Bali dog spermatozoa was not influenced by the freezing procedure.
| The objective of this study was to determined the effects of different extenders on chilled kintamani dog semen quality parameters. In this study, the impact of extenders on motility, viability and DNA integrity of kintamani dog semen were investigated. A total of 12-second fraction of ejaculates were collected from four kintamani dogs using manual stimulation. Sperms were diluted in each of the five extenders at room temperature and then cooled to 4 0 C. Samples were then evaluated every day until five days. Chilled semen samples were assessed for motility, viability and DNA integrity. Results showed that the progressive motility of the sperm cells was significantly higher in extender A and B compared to other extenders. The extender containing 20% egg yolk and 20 % tender coconut water preserved the motility of more than 60% of the spermatozoa up to the day 5 post-sampling. Percentage of live sperm decreased slightly from day 0 to day 5. There was no significant difference in live spermatozoa due to the extender after five day. The DNA was not unaltered by different of extender and storage refrigeration process. It concluded that kintamani dog semen qualities could be maintained for up five days when semen extended with coconut water-based extender with addition fructose and store at 4 0 C. Keywords
There have been studies of the histological structure of male and female goat skins. The histological structure and histomorphometry were taken from two region of skin, which is the thorax and abdomen. The Samples were collected from 2-3 years old Etawah goat, and than was stained using a HarrisHematoxilin Eosin method. The data were analysis based on the region and sex. The results showed that the histological structure of Etawah cross breed consists of three layers were epidermis, dermis, and hipodermis respectively. The epidermis composed by stratum corneum, granulosum, spinosum, and basal. We were found keratin layer on the surface of epidermis. The dermis consists of papillare and reticulare stratum. That was found many sebaceous glands, sudorifera gland, pili muscle, hair follicles, blood vessels, and collagen fibers. The hypodermis layers of Etawah cross breed females skin mostly consists of the fatty tissue, whereas in males we have found thick and horizontally arranged connective tissue. The skin layer on male Etawah cross breed, thorax region thicker than the abdomen. The region and sex may have an effect on the histology structure and histomorphometry of Etawah cross breed skin.
Puberty is controlled by specific physiological mechanisms involving the gonads and adenohypophysis glands, so that puberty does not escape the influence of hereditary factors and the environment that works through these organs (Toelihere, 1995), environment (nutrition, climate and
Kecamatan Gerokgak Kabupaten Buleleng, Bali telah ditetapkan oleh Direktorat Jendral Peternakan Kementrian Pertanian sebagai Pusat Pembibitan Sapi Bali Unggul (PPSBU). Jumlah populasi sapi di Kabupaten Buleleng sebanyak 121.000 ekor, dan 42.000 ekor ada di Kecamatan Gerokgak. Rerata masyarakat memelihara sapi bali 3-4 ekor per keluarga. Sapi tersebut kualitasnya belum diketahui, sehingga perlu dilakukan sertifikasi melalui Lembaga Sertifikasi Produk (Ls-Pro) Benih dan Bibit Sapi Bali, Direktorat Jendral Peternakan Kementrian Pertanian. Kegiatan diawali dengan melakukan pengukuran terhadap 50 ekor sapi bali yang ada di PPSBU Grokgak, dan dikelompokkan ke dalam grade. Sapi bali yang termasuk grade I, dilakukan uji PCR penyakit Jembrana sebagai syarat pengajuan sertifikasi. Hasil kegiatan menunjukkan, 56% sapi bali dikelompokkan ke dalam grade I, 30% grade II dan sebanyak 14% grade III. Sepuluh ekor (20%) bibit sapi bali di PPSBU Gerokgak diajukan sertifikasi.
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